LOCUS       sequence-476584-       11796 bp    DNA     circular SYN 01-JUL-2025
DEFINITION  pLV-U6-[SNCAintron1 gRNA 1]-EFSNC-dSaCas9-KRAB-MeCp2-P2A-Puro-WPRE 
            (THERAPEUTIC VECTOR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11796)
  TITLE     SNCA-targeted epigenome therapy for Parkinson's disease alleviates 
            pathological and behavioral perturbations in a mouse model
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBK1841
FEATURES             Location/Qualifiers
     source          1..11796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2634..2882
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2634..2654
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2805..2824
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2905..2980
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     promoter        3042..3253
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3261..3270
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3270..3335
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3345..3365
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3369..3389
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6663..6848
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             7230..7250
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7269..7325
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7326..7922
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7819..7839
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7938..8526
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7991..8011)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8409..8420)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8598..8831
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8857..8874)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8863..9087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9133..9491
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9220..9239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9430..9451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9509..9528)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9563..9582
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9642..9658)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9642..9658)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9655..9677)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9666..9682
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9690..9696,9697..9714,9715..9720))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9735..9756
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9873..9890)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10044..10632)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10124..10143)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10803..11594,11595..11663))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11426..11445
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11664..11768)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgggcggga cgttaacggg gcggaacggt accgagggcc
     2641 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta
     2701 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     2761 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     2821 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     2881 ccgacctccc agagacctgg cccagtttta gtactctgga aacagaatct actaaaacaa
     2941 ggcaaaatgc cgtgtttatc tcgtcaactt gttggcgaga ttttttgaat tcgctagcta
     3001 ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     3061 cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg
     3121 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     3181 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     3241 cgccagaaca caggaccggt gccaccatgg actacaaaga ccatgacggt gattataaag
     3301 atcatgacat agattacaag gatgacgatg acaagggtgg atcgccaaag aaaaagagga
     3361 aagtcgcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccaagcgga
     3421 actacatcct gggcctggcc atcggcatca ccagcgtggg ctacggcatc atcgactacg
     3481 agacacggga cgtgatcgat gccggcgtgc ggctgttcaa agaggccaac gtggaaaaca
     3541 acgagggcag gcggagcaag agaggcgcca gaaggctgaa gcggcggagg cggcatagaa
     3601 tccagagagt gaagaagctg ctgttcgact acaacctgct gaccgaccac agcgagctga
     3661 gcggcatcaa cccctacgag gccagagtga agggcctgag ccagaagctg agcgaggaag
     3721 agttctctgc cgccctgctg cacctggcca agagaagagg cgtgcacaac gtgaacgagg
     3781 tggaagagga caccggcaac gagctgtcca ccaaagagca gatcagccgg aacagcaagg
     3841 ccctggaaga gaaatacgtg gccgaactgc agctggaacg gctgaagaaa gacggcgaag
     3901 tgcggggcag catcaacaga ttcaagacca gcgactacgt gaaagaagcc aaacagctgc
     3961 tgaaggtgca gaaggcctac caccagctgg accagagctt catcgacacc tacatcgacc
     4021 tgctggaaac ccggcggacc tactatgagg gacctggcga gggcagcccc ttcggctgga
     4081 aggacatcaa agaatggtac gagatgctga tgggccactg cacctacttc cccgaggaac
     4141 tgcggagcgt gaagtacgcc tacaacgccg acctgtacaa cgccctgaac gacctgaaca
     4201 atctcgtgat caccagggac gagaacgaga agctggaata ttacgagaag ttccagatca
     4261 tcgagaacgt gttcaagcag aagaagaagc ccaccctgaa gcagatcgcc aaagaaatcc
     4321 tcgtgaacga agaggatatt aagggctaca gagtgaccag caccggcaag cccgagttca
     4381 ccaacctgaa ggtgtaccac gacatcaagg acattaccgc ccggaaagag attattgaga
     4441 acgccgagct gctggatcag attgccaaga tcctgaccat ctaccagagc agcgaggaca
     4501 tccaggaaga actgaccaat ctgaactccg agctgaccca ggaagagatc gagcagatct
     4561 ctaatctgaa gggctatacc ggcacccaca acctgagcct gaaggccatc aacctgatcc
     4621 tggacgagct gtggcacacc aacgacaacc agatcgctat cttcaaccgg ctgaagctgg
     4681 tgcccaagaa ggtggacctg tcccagcaga aagagatccc caccaccctg gtggacgact
     4741 tcatcctgag ccccgtcgtg aagagaagct tcatccagag catcaaagtg atcaacgcca
     4801 tcatcaagaa gtacggcctg cccaacgaca tcattatcga gctggcccgc gagaagaact
     4861 ccaaggacgc ccagaaaatg atcaacgaga tgcagaagcg gaaccggcag accaacgagc
     4921 ggatcgagga aatcatccgg accaccggca aagagaacgc caagtacctg atcgagaaga
     4981 tcaagctgca cgacatgcag gaaggcaagt gcctgtacag cctggaagcc atccctctgg
     5041 aagatctgct gaacaacccc ttcaactatg aggtggacca catcatcccc agaagcgtgt
     5101 ccttcgacaa cagcttcaac aacaaggtgc tcgtgaagca ggaagaagcc agcaagaagg
     5161 gcaaccggac cccattccag tacctgagca gcagcgacag caagatcagc tacgaaacct
     5221 tcaagaagca catcctgaat ctggccaagg gcaagggcag aatcagcaag accaagaaag
     5281 agtatctgct ggaagaacgg gacatcaaca ggttctccgt gcagaaagac ttcatcaacc
     5341 ggaacctggt ggataccaga tacgccacca gaggcctgat gaacctgctg cggagctact
     5401 tcagagtgaa caacctggac gtgaaagtga agtccatcaa tggcggcttc accagctttc
     5461 tgcggcggaa gtggaagttt aagaaagagc ggaacaaggg gtacaagcac cacgccgagg
     5521 acgccctgat cattgccaac gccgatttca tcttcaaaga gtggaagaaa ctggacaagg
     5581 ccaaaaaagt gatggaaaac cagatgttcg aggaaaagca ggccgagagc atgcccgaga
     5641 tcgaaaccga gcaggagtac aaagagatct tcatcacccc ccaccagatc aagcacatta
     5701 aggacttcaa ggactacaag tacagccacc gggtggacaa gaagcctaat agagagctga
     5761 ttaacgacac cctgtactcc acccggaagg acgacaaggg caacaccctg atcgtgaaca
     5821 atctgaacgg cctgtacgac aaggacaatg acaagctgaa aaagctgatc aacaagagcc
     5881 ccgaaaagct gctgatgtac caccacgacc cccagaccta ccagaaactg aagctgatta
     5941 tggaacagta cggcgacgag aagaatcccc tgtacaagta ctacgaggaa accgggaact
     6001 acctgaccaa gtactccaaa aaggacaacg gccccgtgat caagaagatt aagtattacg
     6061 gcaacaaact gaacgcccat ctggacatca ccgacgacta ccccaacagc agaaacaagg
     6121 tcgtgaagct gtccctgaag ccctacagat tcgacgtgta cctggacaat ggcgtgtaca
     6181 agttcgtgac cgtgaagaat ctggatgtga tcaaaaaaga aaactactac gaagtgaata
     6241 gcaagtgcta tgaggaagct aagaagctga agaagatcag caaccaggcc gagtttatcg
     6301 cctccttcta caacaacgat ctgatcaaga tcaacggcga gctgtataga gtgatcggcg
     6361 tgaacaacga cctgctgaac cggatcgaag tgaacatgat cgacatcacc taccgcgagt
     6421 acctggaaaa catgaacgac aagaggcccc ccaggatcat taagacaatc gcctccaaga
     6481 cccagagcat taagaagtac agcacagaca ttctgggcaa cctgtatgaa gtgaaatcta
     6541 agaagcaccc tcagatcatc aaaaagggca aaaggccggc ggccacgaaa aaggcgctcg
     6601 agggtggagg aagtggcggg tcagggtcgg gtggcagcgc ttcagggtcg ggtggcacta
     6661 gtcggacact ggtgaccttc aaggatgtat ttgtggactt caccagggag gagtggaagc
     6721 tgctggacac tgctcagcag atcgtgtaca gaaatgtgat gctggagaac tataagaacc
     6781 tggtttcctt gggttatcag cttactaagc cagatgtgat cctccggttg gagaagggag
     6841 aagagccctc gggaggtggt tcgggaggtg gttcggaggg tgtgcaggtg aaaagggtcc
     6901 tggagaaaag tcctgggaag ctccttgtca agatgccttt tcaaacttcg ccagggggca
     6961 aggctgaggg gggtggggcc accacatcca cccaggtcat ggtgatcaaa cgccccggca
     7021 ggaagcgaaa agctgaggcc gaccctcagg ccattcccaa gaaacggggc cgaaagccgg
     7081 ggagtgtggt ggcagccgct gccgccgagg ccaaaaagaa agccgtgaag gagtcttcta
     7141 tccgatctgt gcaggagaca gtactcccca tcaagaagcg caagacccgg gaggctagcg
     7201 gaggtggttc gcctagggga ggtggttcgc caaagaagaa acggaaggtg ggccggcccg
     7261 gatccggcgc aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg
     7321 gaccgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     7381 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     7441 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     7501 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     7561 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     7621 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     7681 agcccgcgtg gttcctggcc accgtcggag tctcgcccga ccaccagggc aagggtctgg
     7741 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     7801 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     7861 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     7921 gaacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7981 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     8041 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     8101 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     8161 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     8221 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     8281 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     8341 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8401 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     8461 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     8521 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     8581 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     8641 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8701 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8761 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8821 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     8881 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     8941 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     9001 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     9061 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     9121 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     9181 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     9241 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     9301 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     9361 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     9421 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     9481 cttttgattt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9541 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9601 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     9661 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     9721 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     9781 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     9841 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     9901 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     9961 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10021 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10081 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10141 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10201 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10261 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10321 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10381 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10441 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    10501 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10561 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10621 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10681 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10741 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    10801 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    10861 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    10921 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    10981 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    11041 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    11101 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    11161 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    11221 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    11281 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    11341 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    11401 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    11461 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    11521 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    11581 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    11641 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    11701 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    11761 gttccgcgca catttccccg aaaagtgcca cctgac
//
