LOCUS       sequence-476622-       10130 bp    DNA     circular SYN 18-JUN-2025
DEFINITION  Expresses CDK11A in mammalian cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10130)
  TITLE     On-target toxicity limits the efficacy of CDK11 inhibition against 
            cancers with 1p36 deletions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV[Exp]-Bsd-CMV>hCDK11A
FEATURES             Location/Qualifiers
     source          1..10130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2019..2322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2323..2526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2477..2497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2547..2571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2572..2581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4930..4954)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4972..5560
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5025..5045)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5443..5454)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5591..6090
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5626..5649)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6023..6042
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        6100..6147
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6166..6564
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6651..6884
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6956..7077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6993..7012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7047..7066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7117..7252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7272..7291)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7273..7291)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7301..7318)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7301..7317)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7310..7332)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7459..7914
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7546..7565)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7756..7777
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7940..8044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8045..8113,8114..8905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8263..8282)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9076..9664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9565..9584
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9818..9835
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9952..9973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9988..9993,9994..10011,10012..10018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10026..10042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10031..10053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10050..10066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10050..10066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10085..10105
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10087..10105
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc tgccaccatg
     2581 ggtgatgaaa aggactcttg gaaagtgaaa actttagatg aaattcttca ggaaaagaaa
     2641 cgaaggaagg aacaagagga gaaagcagag ataaaacgct taaaaaattc tgatgaccgg
     2701 gattccaagc gggattccct tgaggagggg gagctgagag atcactgcat ggagatcaca
     2761 ataaggaact ccccgtatag aagagaagac tcaatggaag acagaggaga agaagatgat
     2821 tctttggcca tcaaaccacc ccagcaaatg tctcggaaag aaaaagttca tcacagaaaa
     2881 gatgaaaaga gaaaagaaaa atggaagcat gctagagtga aagaaagaga gcacgaacgt
     2941 cggaaacgac atcgagaaga acaggataaa gctcgccggg aatgggaaag acagaagaga
     3001 agggaaatgg caagggagca ttccaggaga gaaagggacc gcttggagca gttagaaagg
     3061 aagcgggagc gggagcgcaa gatgcgggag cagcagaagg agcagcggga gcagaaggag
     3121 cgcgagcggc gggcggagga gcggcgcaag gagcgggagg cccgcaggga agtgtctgca
     3181 catcaccgaa cgatgagaga ggactacagc gacaaagtga aagccagcca ctggagtcgc
     3241 agcccgcctc ggccgccgcg ggagcggttc gagttgggag acggccggaa gccaggtgag
     3301 gccaggccgg cgcctgcgca gaagccagca cagttaaaag aagagaaaat ggaagaaagg
     3361 gacctgctgt ccgacttaca ggacatcagc gacagcgaga ggaagaccag ctcggccgag
     3421 tcctcgtcag cggaatcagg ctcaggttct gaggaagaag aggaggagga ggaagaggag
     3481 gaggaggaag ggagcaccag tgaagaatca gaggaggaag aggaggaaga ggaagaggag
     3541 gaggaggaga ccggcagcaa ctctgaggag gcatcagagc agtctgccga agaagtaagt
     3601 gaggaagaaa tgagtgaaga tgaagaacga gaaaatgaaa accacctctt ggttgttcca
     3661 gagtcacggt tcgaccgaga ttccggggag agtgaagaag cagaggaaga agtgggtgag
     3721 ggaacgccgc agagcagcgc cctgacagag ggcgactatg tgcccgactc ccctgccctg
     3781 ttgcccatcg agctcaagca ggagctgccc aagtacctgc cggccctgca gggctgccgg
     3841 agcgtcgagg agttccagtg cctgaacagg atcgaggagg gcacctatgg agtggtctac
     3901 agagcaaaag acaagaaaac agatgaaatt gtggctctaa agcggctgaa gatggagaag
     3961 gagaaggagg gcttcccgat cacgtccctg agggagatca acaccatcct caaggcccag
     4021 catcccaaca ttgtcaccgt tagagagatt gtggtgggca gcaacatgga caagatctac
     4081 atcgtgatga actacgtgga gcacgacctc aagagcctga tggagaccat gaaacagccc
     4141 ttcctgccag gggaggtgaa gaccctgatg atccagctgc tgcggggggt gaaacacctg
     4201 cacgacaact ggatcctgca ccgtgacctc aagacgtcca acctgctgct gagccacgcc
     4261 ggcatcctca aggtgggtga ttttgggctg gcgcgggagt acggatcccc tctgaaggcc
     4321 tacaccccgg tcgtggtgac ccagtggtac cgcgccccag agctgctgct tggtgccaag
     4381 gaatactcca cggccgtgga catgtggtca gtgggctgca tcttcgggga gctgctgact
     4441 cagaagcctc tgttccccgg gaattcggaa atcgatcaga tcaacaaagt gttcaaggag
     4501 ctggggaccc ccagtgagaa aatctggccc ggctacagtg agctcccagt agtcaaaaag
     4561 atgaccttca gcgagcaccc ctacaacaac ctccgcaagc gcttcggggc tctgctctca
     4621 gaccagggct tcgacctcat gaacaagttc ctgacctact tccccgggag gaggatcagc
     4681 gctgaggacg gcctcaagca tgagtatttc cgcgagaccc ccctccccat cgacccctcc
     4741 atgttcccca cgtggcccgc caagagcgag cagcagcgtg tgaagcgggg caccagcccg
     4801 aggccccctg agggaggcct gggctacagc cagctgggtg acgacgacct gaaggagacg
     4861 ggcttccacc ttaccaccac gaaccagggg gcctctgccg cgggccccgg cttcagcctc
     4921 aagttctgaa cccagctttc ttgtacaaag tggtgataat cgaattccga taatcaacct
     4981 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5041 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5101 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5161 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5221 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5281 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5341 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     5401 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5461 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5521 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgggaatt cccgcggttc
     5581 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     5641 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     5701 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     5761 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     5821 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     5881 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     5941 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     6001 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     6061 ttctcctctt cctcatctcc gggcctttcg acctcacgtg ttgacaatta atcatcggca
     6121 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg
     6181 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     6241 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     6301 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     6361 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     6421 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     6481 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     6541 tctggttatg tgtgggaggg ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac
     6601 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6661 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6721 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6781 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6841 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6901 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6961 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7021 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7081 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7141 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7201 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     7261 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     7321 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7381 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7441 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7501 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7561 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7621 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7681 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7741 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7801 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7861 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7921 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7981 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8041 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8101 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8161 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8221 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8281 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8341 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8401 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8461 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8521 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8581 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8641 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8701 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8761 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8821 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8881 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8941 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9001 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9061 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9121 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9181 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9241 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9301 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9361 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9421 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9481 cacgcttccc gaagagagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9541 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9601 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9661 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9721 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9781 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9841 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     9961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    10021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
    10081 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
//
