LOCUS       sequence-476822-       10166 bp    DNA     circular SYN 23-JUN-2025
DEFINITION  Expresses CDK11B with D562A kinase inactivating mutation and G579S 
            resistance mutation in mammalian cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10166)
  TITLE     On-target toxicity limits the efficacy of CDK11 inhibition against 
            cancers with 1p36 deletions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV[Exp]-Bsd-CMV>hCDK11B D562A, G579S
FEATURES             Location/Qualifiers
     source          1..10166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2019..2322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2323..2526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2477..2497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2547..2571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2572..2581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4966..4990)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5008..5596
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5061..5081)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5479..5490)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5627..6126
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5662..5685)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6059..6078
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        6136..6183
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6202..6600
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6687..6920
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6992..7113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7029..7048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7083..7102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7153..7288
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7308..7327)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7309..7327)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7337..7354)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7337..7353)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7346..7368)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7495..7950
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7582..7601)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7792..7813
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7976..8080
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8081..8149,8150..8941)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8299..8318)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9112..9700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9601..9620
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9854..9871
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9988..10009
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10024..10029,10030..10047,10048..10054)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10062..10078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10067..10089
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10086..10102
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10086..10102
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10121..10141
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10123..10141
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggag cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc tgccaccatg
     2581 ggtgatgaaa aggactcttg gaaagtgaaa actttagatg aaattcttca ggaaaagaaa
     2641 cgaaggaagg aacaagagga gaaagcagag ataaaacgct taaaaaattc tgatgaccgg
     2701 gattccaagc gggattccct tgaggagggg gagctgagag atcaccgcat ggagatcaca
     2761 ataaggaact ccccgtatag aagagaagac tctatggaag acagaggaga agaagatgat
     2821 tctttggcca tcaaaccacc ccagcaaatg tctcggaaag aaaaagctca tcacagaaaa
     2881 gatgaaaaga gaaaagagaa acgtaggcat cgtagccatt cagcagaagg ggggaagcat
     2941 gctagagtga aagaaaaaga aagagagcac gaacgtcgga aacggcatcg agaagaacag
     3001 gataaagctc gccgggaatg ggaaagacag aagagaaggg agatggcaag ggagcattcc
     3061 aggagagaaa gggaccgctt ggagcagtta gaaaggaagc gggagcggga gcgcaagatg
     3121 cgggagcagc agaaggagca gcgggagcag aaggagcgcg agcggcgggc agaggagcgg
     3181 cgcaaggagc gggaggcccg cagggaagtg tctgcacatc accgaacgat gagagaggac
     3241 tacagcgaca aagtgaaagc cagccactgg agtcgcagcc cgcctcggcc gccgcgggag
     3301 cggttcgagt tgggagacgg ccggaagcca ggtgaggcca ggccggcgcc tgcgcagaag
     3361 ccagcacagt taaaagaaga gaaaatggaa gaaagggacc tgctgtccga cttacaggac
     3421 atcagcgaca gcgagaggaa gaccagctcg gccgagtcct cgtcagcgga atcaggctca
     3481 ggttctgagg aagaagagga ggaggaggaa gaggaggagg aggaagggag caccagtgaa
     3541 gaatcagagg aggaggagga ggaagaggaa gaggaggagg aggagaccgg cagcaactct
     3601 gaggaggcat cagagcagtc tgccgaagaa gtaagtgagg aagaaatgag tgaagatgaa
     3661 gaacgagaaa atgaaaacca cctcttggtt gttccagagt cacggttcga ccgagattcc
     3721 ggggagagtg aagaagcaga ggaagaagtg ggtgagggaa cgccgcagag cagcgccctg
     3781 acagagggcg actatgtgcc cgactcccct gccctgtcgc ccatcgagct caagcaggag
     3841 ctgcccaagt acctgccggc cctgcagggc tgccggagcg tcgaggagtt ccagtgcctg
     3901 aacaggatcg aggagggcac ctatggagtg gtctacagag caaaagacaa gaaaacagat
     3961 gaaattgtgg ctctaaagcg gctgaagatg gagaaggaga aggagggctt cccgatcacg
     4021 tcgctgaggg agatcaacac catcctcaag gcccagcatc ccaacatcgt caccgttaga
     4081 gagattgtgg tgggcagcaa catggacaag atctacatcg tgatgaacta tgtggagcac
     4141 gacctcaaga gcctgatgga gaccatgaaa cagcccttcc tgccagggga ggtgaagacc
     4201 ctgatgatcc agctgctgcg tggggtgaaa cacctgcacg acaactggat cctgcaccgt
     4261 gccctcaaga cgtccaacct gctgctgagc cacgccggca tcctcaaggt gagtgacttc
     4321 gggctggcgc gggagtacgg atcccctctg aaggcctata ccccggtcgt ggtgaccctg
     4381 tggtaccgcg ccccagagct gctgcttggt gccaaggaat actccacggc cgtggacatg
     4441 tggtcagtgg gttgcatctt cggggagctg ctgactcaga agcctctgtt ccccgggaag
     4501 tcagaaatcg atcagatcaa caaggtgttc aaggatctgg ggacccctag tgagaaaatc
     4561 tggcccggct acagcgagct cccagcagtc aagaagatga ccttcagcga gcacccctac
     4621 aacaacctcc gcaagcgctt cggggctctg ctctcagacc agggcttcga cctcatgaac
     4681 aagttcctga cctacttccc cgggaggagg atcagcgctg aggacggcct caagcatgag
     4741 tatttccgcg agacccccct ccccatcgac ccctccatgt tccccacgtg gcccgccaag
     4801 agcgagcagc agcgtgtgaa gcggggcacc agcccgaggc cccctgaggg aggcctgggc
     4861 tacagccagc tgggtgacga cgacctgaag gagacgggct tccaccttac caccacgaac
     4921 cagggggcct ctgccgcggg ccccggcttc agcctcaagt tctgaaccca gctttcttgt
     4981 acaaagtggt gataatcgaa ttccgataat caacctctgg attacaaaat ttgtgaaaga
     5041 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     5101 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     5161 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     5221 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     5281 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     5341 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     5401 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     5461 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     5521 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     5581 tgggccgcct ccccgcatcg ggaattcccg cggttcgaat tctaccgggt aggggaggcg
     5641 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     5701 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     5761 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     5821 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     5881 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     5941 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     6001 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     6061 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     6121 ctttcgacct cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     6181 gacaaggtga ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt
     6241 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     6301 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     6361 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     6421 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     6481 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     6541 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     6601 gcacaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6661 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6721 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6781 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6841 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6901 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     6961 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7021 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7081 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7141 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7201 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7261 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     7321 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7381 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     7441 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     7501 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7561 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7621 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7681 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7741 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7801 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7861 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7921 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     7981 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8041 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8101 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8161 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8221 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8281 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8341 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8401 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8461 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8521 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8581 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8641 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8701 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8761 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8821 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8881 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8941 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9001 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9061 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9121 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9181 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9241 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9301 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9361 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9421 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9481 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag agagaaaggc
     9541 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9601 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9661 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9721 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9781 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9841 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9901 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     9961 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    10021 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    10081 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
    10141 ggaacaaaag ctggagctgc aagctt
//
