LOCUS       sequence-476993-       10651 bp    DNA     circular SYN 25-JUN-2025
DEFINITION  Expresses of GFP-tagged mTet1s-delCys in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Arroyo M, Hastert FD, Zhadan A, Schelter F, Zimbelmann S, Rausch C, 
            Ludwig AK, Carell T, Cardoso MC
  TITLE     Isoform-specific and ubiquitination dependent recruitment of Tet1 to
            replicating heterochromatin modulates methylcytosine oxidation.
  JOURNAL   Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8.
  PUBMED    36056023
REFERENCE   2  (bases 1 to 10651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-2"; volume: "13(1):5173. doi"; pages: " 
            10.1038/s41467-022-32799-8"
COMMENT     Sequence Label: pGFP-mTet1s-delCys  pc3904
FEATURES             Location/Qualifiers
     source          1..10651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3842..4304
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3898..3915)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4125..4144
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      4309..4318
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4315..4713
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4871..4992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4908..4927)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4962..4981
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5040..5175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5281..5298)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5452..6040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5532..5551)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6211..7002,7003..7071))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6834..6853
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7072..7176)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7244..7262
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7300..7322)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7422..7441
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7508..7963)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7645..7666)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7857..7876
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8089..8111
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8103..8120
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8104..8120
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8127..8145
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        8214..8517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8519..8795
                     /label=chicken beta-actin promoter
     intron          8796..9812
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     9736..9758
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     9820..9839
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      9884..9893
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9890..9892,9893..9895,9896..10606)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9890..9892,9893..9895,9896..10606)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9935..9956)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10196..10215)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10543..10564
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10613..10633
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atggactgca gtagacgtgg tcatggggaa gaagagcaaa ggttggattt gatcacacat
       61 ccccttgaaa atgtaaggaa gaatgcaggc ggcatgacgg gaattgaggt ggagaagtgg
      121 gcgccaaaca agaaatcaca cttagcagag ggtcaagtca aaggaagctg tgatgccaat
      181 ttaactgggg tggaaaaccc ccagccctct gaagatgaca agcagcaaac caacccttct
      241 ccgacatttg cccagaccat aaggaacggc atgaaaaatg tacactgttt acccactgac
      301 acacaccttc cgctcaacaa attgaatcat gaggaattta gcaaggcgtt gggaaataat
      361 tcctctaaat tgctgactga tccctcaaac tgcaaggatg ccatgagtgt caccacctcc
      421 gggggggagt gtgatcatct caaggggcca aggaacaccc tgctcttcca gaagcctggc
      481 ttgaactgca ggtctggtgc agaacccacc atctttaata atcaccctaa tacacacagc
      541 gctgggagtc gacctcaccc tcctgagaag gtacccaaca aagaacccaa agatggctct
      601 ccggttcagc caagtcttct atccctaatg aaggacagga gattaacgct ggaacaagtg
      661 gtagccatag aagccctgac tcagctctca gaagcgccct cagagagctc ctccccatcc
      721 aagccggaga aggatgaaga agcgcatcag aaaacggcta gtctgcttaa cagttgcaag
      781 gctatccttc attcggtgag aaaagacctc caagacccaa acgttcaagg aaagggcctt
      841 caccatgaca ctgttgtctt taatggccag aacagaacat tcaagtcacc tgatagtttc
      901 gctactaacc aagcacttat aaaatcacaa ggatatccaa gttcacctac agctgagaag
      961 aaaggcgctg ctggtgggag agctcccttt gatggttttg agaattcaca tccattgccc
     1021 atagaaagtc acaatcttga aaactgcagt caggtactaa gctgcgatca gaatttgagt
     1081 tcccatgacc catcatgcca ggatgcaccc tacagtcaaa ttgaagaaga tgtcgcagca
     1141 caattgaccc aacttgcatc cacgattaat cacatcaacg ccgaggtcag gaatgctgaa
     1201 agtacaccag aaagccttgt tgcaaagaac acaaagcaaa aacacagtca ggagaaacgc
     1261 atggtacatc agaagccacc ctctagtaca caaactaaac ccagtgtacc atcagcaaag
     1321 ccaaagaagg cccagaaaaa ggcaagagcg accccacacg caaacaagcg gaaaaagaaa
     1381 cccccagccc gaagctctca agaaaatgat cagaagaagc aagagcagct agctatagag
     1441 tatagtaaaa tgcatgacat ttggatgtca tctaagtttc aaaggtttgg ccagtctagt
     1501 ccacgtagtt ttcctgttct gttgagaaat attcctgtct ttaaccaaat attaaagccc
     1561 gtgactcaga gtaaaacacc ctcacaacat aatgagttat ttcctcctat caatcagata
     1621 aaattcacaa gaaaccctga attggcaaag gagaaagtga aggttgaacc atcagattct
     1681 ctgccaacat gccaattcaa gacagaatcc ggtgggcaga catttgcaga gccggcagat
     1741 aattctcagg gacagcccat ggtgagtgtc aatcaggaag ctcaccctct gccccagtcc
     1801 ccaccatcta accagtgtgc taatataatg gctggtgctg cccaaacaca gtttcatcta
     1861 ggcgctcaag agaacctggt gcatcagata ccaccaccga cgttgcctgg tacctcccct
     1921 gacacactct tgccagaccc agcatcaatc ctcaggaaag ggaaagtgtt acatttcgat
     1981 ggaattacag ttgttacgga gaagcgtgaa gctcaaacat caagcaatgg accactgggt
     2041 cccaccacag acagtgcaca gtcagaattt aaagagagca tcatggactt acttagcaag
     2101 cctgcaaaaa acctgatagc tggattgaag gaacagagga gtgaaaaccc cagaaaattc
     2161 agacttgctc caaactaccc cttacataac tactataaga gaattactgg aatgagttct
     2221 gaaggaagtg acgtgaaaac cgggtggatc attccagacc gcaagaccct cataagcaga
     2281 gaggaaaaac agcttgaaaa gaatttacaa gaattggcta cagtattagc tccactttac
     2341 aagcagatgg ctccagttgc ttatcaaaat caggtggaat atgaagaagt tgctggagac
     2401 tgtcgacttg gaaatgaaga ggggcgtcct ttctctggtg tcacctgttg catggatttt
     2461 tgtgcccatt ctcacaagga cattcacaac atgcacaacg gaagcaccgt ggtgtgtacg
     2521 ttgattcgag cagatggccg tgacacaaat tgtcccgagg atgaacaact ccacgtcctg
     2581 ccactatacc ggcttgcaga cactgatgaa tttggctccg tggaagggat gaaggccaaa
     2641 atcaaatctg gggccatcca agtcaatggg ccaaccagga agaggcgact acgttttact
     2701 gagcctgttc ctcgatgtgg gaagagggcc aaaatgaagc agaaccacaa taaatcaggt
     2761 actgctggtc tcagaaggaa gaggatctct gccagcccaa agggagcccc aggttcacac
     2821 aacactaaga gcttttcatc agcctcatct acttctcacc tagtgaaaga cgaatctaca
     2881 gacttctgtc ccctgcaggc ttcctccgca gaaacatcta cctgtacgta cagtaaaaca
     2941 gcctcaggtg ggtttgcaga aacaagtagt attctccact gcacaatgcc ttctggagca
     3001 cacagtggtg ctaatgcagc tgctggggaa tgtactggaa cggtgcagcc tgccgaggtg
     3061 gctgctcatc ctcaccagtc tcttcccaca gccgattctc ccgttcatgc tgagcctctc
     3121 actagtccat ctgagcagct aacttctaac cagtcaaacc agcagctccc tctcctcagc
     3181 aattctcaga aactggcttc ctgtcaggtg gaagatgagc ggcaccctga agcggatgag
     3241 cctcagcacc ccgaggacga taacttgcct caacttgatg aattctggtc agacagtgag
     3301 gagatctacg ccgatccttc ctttggtggc gtggcgatag cacccattca cggctcggtg
     3361 ctcattgagt gcgctcggaa ggagcttcat gctaccacct ctttgcgctc ccccaaacga
     3421 ggggtccctt ttcgtgtgtc ccttgtattc taccagcaca aaagcctaaa caagcctaat
     3481 catggttttg atatcaacaa aattaagtgt aaatgcaaaa aagtaacgaa aaaaaagccc
     3541 gcagaccggg agtgtcctga tgtatccccc gaagccaatt tatcacacca aattccttct
     3601 cgagttgcat caaccttaac ccgagacaat gttgttaccg tgtccccata ctctctcact
     3661 catgttgcgg gaccctacaa tcgttgggtc taagcggccg cagttaacga attccgcccc
     3721 cccccccccc ccccycyccc yccccccccc ctaacgttac tggccgaagc cgcttggaat
     3781 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     3841 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     3901 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     3961 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     4021 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     4081 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     4141 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     4201 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     4261 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc caccatggcc
     4321 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc
     4381 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc
     4441 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg
     4501 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag
     4561 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct
     4621 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa
     4681 ttgctgccct ctggttatgt gtgggagggc taagcacttc gtggccgagg agcaggactg
     4741 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4801 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4861 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4921 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4981 caatgtatct tatcatgtct gtataccgtc gacctcgagg agctttttgc aaaagcctag
     5041 gcctccaaaa aagcctcttc actacttctg gaatagctca gaggccgagg cggcctcggc
     5101 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     5161 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgctgca
     5221 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5281 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5341 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5401 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5461 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5521 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5581 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5641 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5701 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5761 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5821 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5881 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5941 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6001 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6061 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6121 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6181 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6241 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6301 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6361 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6421 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6481 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6541 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6601 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6661 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6721 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6781 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6841 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6901 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6961 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7021 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7081 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7141 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7201 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     7261 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     7321 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     7381 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     7441 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7501 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     7561 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     7621 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     7681 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     7741 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     7801 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     7861 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     7921 accaccacac ccgccgcgct caatgcgccg ctacagggcg cgtccattcg ccattcaggc
     7981 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     8041 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     8101 gttgtaaaac gacggccagt gaattgtaat acgactcact ataggacgcg tcctagttat
     8161 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     8221 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     8281 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     8341 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     8401 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     8461 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     8521 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     8581 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggc
     8641 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
     8701 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     8761 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
     8821 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
     8881 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
     8941 aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc
     9001 ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg
     9061 cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg
     9121 cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     9181 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     9241 tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg
     9301 gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg
     9361 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     9421 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     9481 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     9541 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     9601 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     9661 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     9721 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     9781 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     9841 tgtctcatca ttttggcaaa gaattcggat ccaccggccg gtcgccacca tggtgagcaa
     9901 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     9961 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
    10021 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
    10081 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
    10141 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
    10201 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
    10261 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
    10321 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
    10381 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
    10441 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
    10501 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
    10561 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg gagaaaacct
    10621 atacttccaa ggaagatctc gaggcgatcg c
//
