LOCUS       sequence-477063-       15326 bp    DNA     circular SYN 25-JUN-2025
DEFINITION  Ribozyme split sfGFP linked with GFPuv via T2A expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15326)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 15326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB162
FEATURES             Location/Qualifiers
     source          1..15326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1769..1797)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2805..2825
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2875..2931
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      3593..3841
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(4531..4547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4531..4547)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4544..4566)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4555..4571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4579..4585,4586..4603,4604..4609))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4624..4645
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4836..5512
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             5580..6377
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5637..5656)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6247..6266
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6417..6591
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6669..6693
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7118..7912
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7999..8587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8488..8507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8741..8758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8773..8913
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8839..8858)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8999..9021
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9257..9451
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9517..10590)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11019..11648)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12949..12973
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(12995..13247)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(13279..13296)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(13303..15120,15311..15325))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          15121..15310
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1801 tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa
     1861 actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc caacacttgt
     1921 cactactctg acctaaatag caatatttac ctttgggtca aaaagttatc aggcatgcac
     1981 ctggtagcta gtctttaaac caatagattg catcggttta aaaggcaaga ccgtcaaatt
     2041 gcgggaaagg ggtcaacagc cgttcagtac caagtctcag gggaaacttt gagatggcct
     2101 tgcaaagggt atggtaataa gctgacggac atggtcctaa ccacgcagcc aagtcctaag
     2161 tcaacagatc ttctgttgat atggatgcag ttcacagact aaatgtcggt cggggaagat
     2221 gtattcttct cataagatat agtcggacct ctccttaatg ggagctagcg gatgaagtga
     2281 tgcaacactg gagccgctgg gaactaattt gtatgcgaaa gtatattgat tagttttgga
     2341 gtactcgatg gtgttcaatg cttttcccgt tatccggatc acatgaaacg gcatgacttt
     2401 ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac
     2461 gggacctaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc
     2521 gagttaaagg gtattgattt taaagaagat ggaaacattc ttggacacaa actcgagtac
     2581 aactttaact cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct
     2641 aacttcaaaa ttcgccacaa cgttgaagat ggttccgttc aactagcaga ccattatcaa
     2701 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca
     2761 caatctgtcc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct tcttgagttt
     2821 gtaactgctg ctgggattac acatggcatg gatgagctct acaaaggaag cggagctact
     2881 aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc tatgacaacc
     2941 ttcaaaatcg agtcccggat ccacggcaac ctcaacgggg agaagttcga gttggttgga
     3001 ggtggagtag gtgaggaggg tcgcctcgag attgagatga agactaaaga taaaccactg
     3061 gcattctctc ccttcctgct gaccacttgc atgggttacg ggttctacca cttcgccagc
     3121 ttcccaaagg ggattaagaa catctatctt catgctgcaa cgaacggagg ttacaccaac
     3181 accaggaagg agatctatga agacggcggc atcttggagg tcaacttccg ttacacttac
     3241 gagttcaaca agatcatcgg tgacgtcgag tgcattggac atggattccc aagtcagagt
     3301 ccgatcttca aggacacgat cgtgaagagt tgtcccacgg tggacctgat gttgccaatg
     3361 tccgggaaca tcatcgccag ctcctacgct tacgccttcc aactgaagga cggctctttc
     3421 tacacggcag aagtcaagaa caacatagac ttcaagaatc caatccacga gtccttctcg
     3481 aagtcagggc ccatgttcac ccacagacgt gtcgaggaga ctctcaccaa ggagaacctt
     3541 gccatagtgg agtaccagca ggttttcaac agcgccccaa gagacatgta gatatgaaga
     3601 tgaagatgaa atatttggtg tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt
     3661 tcttggcttg ttgtgttatg aatttgtggc tttttctaat attaaatgaa tgtaagatct
     3721 cattataatg aataaacaaa tgtttctata atccattgtg aatgttttgt tggatctctt
     3781 ctgcagcata taactactgt atgtgctatg gtatggacta tggaatatga ttaaagataa
     3841 gatgggctca tagagtaaaa cgaggcgagg gacctataaa cctcccttca tcatgctatt
     3901 tcatgatcta ttttataaaa taaagatgta gaaaaaagta agcgtaataa ccgcaaaaca
     3961 aatgatttaa aacatggcac ataatgagga gattaagttc ggtttacgtt tattttagta
     4021 ctaattgtaa cgtgagacta cgtatcggga atcgcctaat taaagcatta atgcgaacct
     4081 gattagattc accgaccctc ctatcgtgtc gacctttctg tttcttagaa ttttttggta
     4141 gtctatgtac taataatgtc agcttcgtat ttatttcata agcaatttgc atttgcaatt
     4201 tgttttttac ttttattttt attgtattgt ggaatgtgga ctcgtaccaa catgaagtta
     4261 tataccacca aaaaaattac agttagtcaa aagattcacg agtgagagct acttatgatt
     4321 gtcttttacg tatatgtcta attgtctatt tgctcaataa tctttgtact ttcttttgtc
     4381 gttgataaaa tcacaaagtt ccaaaagtaa tcgaatgatt tgcttttaag aaaagaagag
     4441 ctcaataatt caacatatat ctgtacacaa ttaccgcctt tgagtgagct ctagatgcca
     4501 cctgacgtct aagaagaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4561 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4621 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4681 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4741 tattggctag agcagcttgc caacatggtg gagcacgaca ctctcgtcta ctccaagaat
     4801 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
     4861 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
     4921 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
     4981 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     5041 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgaaca tggtggagca
     5101 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat
     5161 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
     5221 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
     5281 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
     5341 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5401 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5461 agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct gaaatcacca
     5521 gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg caatgagata
     5581 tggggattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     5641 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     5701 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     5761 tccaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     5821 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     5881 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     5941 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     6001 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     6061 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     6121 acggcgagga tctcgtcgtg acacatggcg atgcctgctt gccgaatatc atggtggaaa
     6181 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     6241 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     6301 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     6361 ttgacgagtt cttctgaagt agatgccgac cggatctgtc gatcgacaag ctcgagtttc
     6421 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
     6481 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     6541 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt
     6601 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     6661 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     6721 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     6781 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     6841 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     6901 aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata
     6961 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
     7021 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
     7081 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
     7141 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
     7201 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
     7261 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
     7321 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
     7381 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
     7441 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
     7501 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
     7561 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
     7621 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
     7681 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
     7741 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
     7801 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
     7861 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga
     7921 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7981 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8041 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8101 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     8161 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8221 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8281 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8341 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8401 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8461 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8521 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8581 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8641 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8701 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8761 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     8821 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     8881 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     8941 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     9001 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     9061 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     9121 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     9181 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     9241 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     9301 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     9361 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     9421 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     9481 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     9541 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     9601 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     9661 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     9721 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     9781 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     9841 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     9901 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     9961 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
    10021 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
    10081 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
    10141 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
    10201 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
    10261 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
    10321 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
    10381 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
    10441 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
    10501 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
    10561 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
    10621 ccattgcagg gccggcaggc aacccagccg cttacgcctg gccaaccgcc cgttcctcca
    10681 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
    10741 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
    10801 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
    10861 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
    10921 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
    10981 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
    11041 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
    11101 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
    11161 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
    11221 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
    11281 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    11341 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    11401 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    11461 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    11521 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    11581 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    11641 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    11701 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    11761 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    11821 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    11881 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    11941 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    12001 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    12061 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    12121 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    12181 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    12241 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    12301 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    12361 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    12421 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    12481 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    12541 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    12601 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    12661 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    12721 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    12781 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc
    12841 ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca
    12901 cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt ttacccgcca
    12961 atatatcctg tcaaacactg atagtttaat tcccgatcta gtaacataga tgacaccgcg
    13021 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
    13081 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
    13141 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
    13201 acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg gaaattcgag
    13261 ctggtcacca attcacacgt gatggtgatg gtgatggcta gcgttcttgt agccgaaatc
    13321 tggaatgttg gtccagcgct cgcgaaagac gtgcgcggcg agcttcggct tgcggtcacg
    13381 agtgaacacg cccttcttgt ttccttggac gcgcatcacg ccctgagagg tcgcgaagtc
    13441 cgcgaagttc cacgcttgct cacccacgaa gttctcaaac tcatcgaaca cgacgtggtt
    13501 cgcctggtag tactcgactt gatattcctc ggtgaacatc actggatcaa tgtcgtgaaa
    13561 gcccgcaacg gtgtctgcgc cgtactcagt gatcatgatc ggctttcctg ggcaacgctt
    13621 gttccacgcg tgaaattcct ggcggagatg gactttggcc gcttcgagat caccgccatc
    13681 gaagtaccat ccgttatagc gattgagcgc gatgacgtca atcagttcgg cgactttgtc
    13741 cgtctccggg gtagccatca caaacagcac gatcgtgacc ggacgcttct gtgggtcgag
    13801 ttccttggtc agctccacca acggcttgaa gtactcgtac gcgccctctt cctcagtcgc
    13861 cgcctcgttg gcgatgctcc acatcacgac gcttggatgg ttcttgtcac gagacaccag
    13921 ttcacggaga acgtcttgat ggtgctcaaa cgtccgaatc ttctcccagg tactgacgcg
    13981 ctcgctgcct tcgccgagtc ccgtggtggc catgaagttg aggtgcacgc caactgccgg
    14041 agtctcgtcg atcacgacca gaccctcgcg atccgcaaga cgcatcaact cttcagagta
    14101 cggatagtgt gcggtccgga agctgttggc gccgatccat ttgaggatat tgaaatccat
    14161 cacattgctc gcttcgttaa agccacggcc gttgatagga gtgtcctcat gtttgccaaa
    14221 gcccttgaag tagaacggtt tgttgttgat gaggaacttg ccgtcgttga cttccacggt
    14281 ccgcacgccg aacggctctt catagacatc gatggtcagt ccgtcgttca ccagttccac
    14341 tttgatctgg tagagatacg tgttcagtgg ttcccagagg atgacattcg gaatctccac
    14401 gttaccgctc aggccctcgg tgcttgcgac cactttgcct tcctcatcca cgaccgacac
    14461 tttcacggtc tcggctttgc cttgaaagtc caccgtatag gtcacagtcc cggttgggcc
    14521 attgaagtcg gtcacaaccg agatgtcctc gacgtacgta aacggggtcg tgtagatttt
    14581 caccggacgg tgcaggcctg catagttgaa gaagtcgaag ttcggcttgt tacgaatgac
    14641 ttttccgagg ccctcttcgt ggcgctcgct gtacagcccc accgggaggg tgctatcgtc
    14701 gaggatgttg tccacggcga cggtgacgcg attcatgcca tcacgcagcg agttgttgat
    14761 ttccgcttcg aatggcagga atccgccctt gtgctccacg accagctcac cattgacata
    14821 gacaattgct ttgtgagttg cagagccgaa gcggagcacg atacgctgat ccttcagata
    14881 ggccggcacc gtgaactcac gttcgtacca gacatatccg atatggttgc ggatttcctt
    14941 ggtcacgcca atgtcattgt aactgcttgg gacggccata ctaatagtgt cggtcagctt
    15001 gctttcgtac cacttctctt ccagtccttt cccgtagtcc agcttgaagt tccagacgcc
    15061 attgaggtcg aagacgccac gggtctcggt gttgatcggg tacagactag ttcgtcggtt
    15121 ctgtaactat catcatcatc atagacacac gaaataaagt aatcagatta tcagttaaag
    15181 ctatgtaata tttacaccat aaccaatcaa ttaaaaaata gatcagttta aagaaagatc
    15241 aaagctcaaa aaaataaaaa gagaaaaggg tcctaaccaa gaaaatgaag gagaaaaact
    15301 agaaatttac cctcagatct accatg
//
