LOCUS       sequence-477066-       14243 bp    DNA     circular SYN 25-JUN-2025
DEFINITION  Expression vector of GFPuv with 3' Triplex motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14243)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB111
FEATURES             Location/Qualifiers
     source          1..14243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2510..2758
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3448..3464)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3448..3464)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3461..3483)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3472..3488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3496..3502,3503..3520,3521..3526))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3541..3562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3753..4429
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4497..5294
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4554..4573)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5164..5183
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5334..5508
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5586..5610
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6035..6829
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6916..7504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7405..7424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7658..7675
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7690..7830
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7756..7775)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7916..7938
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8174..8368
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8434..9507)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9936..10565)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11866..11890
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11912..12164)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12196..12213)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12220..14037,14228..14242))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14038..14227
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctatcttcat gctgcaacga acggaggtta
     1981 caccaacacc aggaaggaga tctatgaaga cggcggcatc ttggaggtca acttccgtta
     2041 cacttacgag ttcaacaaga tcatcggtga cgtcgagtgc attggacatg gattcccaag
     2101 tcagagtccg atcttcaagg acacgatcgt gaagagttgt cccacggtgg acctgatgtt
     2161 gccaatgtcc gggaacatca tcgccagctc ctacgcttac gccttccaac tgaaggacgg
     2221 ctctttctac acggcagaag tcaagaacaa catagacttc aagaatccaa tccacgagtc
     2281 cttctcgaag tcagggccca tgttcaccca cagacgtgtc gaggagactc tcaccaagga
     2341 gaaccttgcc atagtggagt accagcaggt tttcaacagc gccccaagag acatgtagga
     2401 ttcgtcagta gggttgtaaa ggtttttctt ttcctgagaa aacaaccttt tgttttctca
     2461 ggttttgctt tttggccttt ccctagcttt aaaaaaaaaa aagcaaaaat atgaagatga
     2521 agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct
     2581 tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aagatctcat
     2641 tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttctg
     2701 cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta aagataagat
     2761 gggctcatag agtaaaacga ggcgagggac ctataaacct cccttcatca tgctatttca
     2821 tgatctattt tataaaataa agatgtagaa aaaagtaagc gtaataaccg caaaacaaat
     2881 gatttaaaac atggcacata atgaggagat taagttcggt ttacgtttat tttagtacta
     2941 attgtaacgt gagactacgt atcgggaatc gcctaattaa agcattaatg cgaacctgat
     3001 tagattcacc gaccctccta tcgtgtcgac ctttctgttt cttagaattt tttggtagtc
     3061 tatgtactaa taatgtcagc ttcgtattta tttcataagc aatttgcatt tgcaatttgt
     3121 tttttacttt tatttttatt gtattgtgga atgtggactc gtaccaacat gaagttatat
     3181 accaccaaaa aaattacagt tagtcaaaag attcacgagt gagagctact tatgattgtc
     3241 ttttacgtat atgtctaatt gtctatttgc tcaataatct ttgtactttc ttttgtcgtt
     3301 gataaaatca caaagttcca aaagtaatcg aatgatttgc ttttaagaaa agaagagctc
     3361 aataattcaa catatatctg tacacaatta ccgcctttga gtgagctcta gatgccacct
     3421 gacgtctaag aagaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     3481 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     3541 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3601 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3661 tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc
     3721 aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg
     3781 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa
     3841 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
     3901 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     3961 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga
     4021 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
     4081 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
     4141 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
     4201 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     4261 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     4321 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     4381 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc
     4441 tctctctaca aatctatctc tctcgagctt tcgcagatcc cggggggcaa tgagatatgg
     4501 ggattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4561 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4621 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc
     4681 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     4741 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     4801 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     4861 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     4921 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     4981 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     5041 gcgaggatct cgtcgtgaca catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5101 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5161 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5221 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5281 acgagttctt ctgaagtaga tgccgaccgg atctgtcgat cgacaagctc gagtttctcc
     5341 ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca
     5401 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     5461 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatccc ccgaattaat
     5521 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     5581 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     5641 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     5701 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     5761 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     5821 gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta
     5881 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
     5941 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
     6001 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
     6061 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
     6121 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
     6181 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
     6241 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
     6301 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
     6361 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
     6421 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
     6481 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
     6541 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
     6601 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     6661 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
     6721 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     6781 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt acctagaatg
     6841 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6901 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6961 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7021 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     7081 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7141 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7201 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7261 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7321 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7381 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7441 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7501 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7561 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7621 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7681 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     7741 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     7801 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     7861 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     7921 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
     7981 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
     8041 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     8101 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     8161 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     8221 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     8281 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     8341 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     8401 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     8461 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     8521 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     8581 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     8641 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     8701 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     8761 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     8821 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     8881 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
     8941 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
     9001 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
     9061 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
     9121 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
     9181 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
     9241 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
     9301 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
     9361 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
     9421 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
     9481 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
     9541 ttgcagggcc ggcaggcaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
     9601 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
     9661 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
     9721 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
     9781 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
     9841 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
     9901 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
     9961 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
    10021 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
    10081 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
    10141 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
    10201 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
    10261 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
    10321 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
    10381 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
    10441 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
    10501 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
    10561 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
    10621 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
    10681 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
    10741 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
    10801 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
    10861 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
    10921 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
    10981 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    11041 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    11101 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    11161 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    11221 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    11281 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    11341 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    11401 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    11461 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    11521 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    11581 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    11641 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    11701 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg
    11761 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
    11821 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
    11881 tatcctgtca aacactgata gtttaattcc cgatctagta acatagatga caccgcgcgc
    11941 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat
    12001 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt
    12061 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca
    12121 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa attcgagctg
    12181 gtcaccaatt cacacgtgat ggtgatggtg atggctagcg ttcttgtagc cgaaatctgg
    12241 aatgttggtc cagcgctcgc gaaagacgtg cgcggcgagc ttcggcttgc ggtcacgagt
    12301 gaacacgccc ttcttgtttc cttggacgcg catcacgccc tgagaggtcg cgaagtccgc
    12361 gaagttccac gcttgctcac ccacgaagtt ctcaaactca tcgaacacga cgtggttcgc
    12421 ctggtagtac tcgacttgat attcctcggt gaacatcact ggatcaatgt cgtgaaagcc
    12481 cgcaacggtg tctgcgccgt actcagtgat catgatcggc tttcctgggc aacgcttgtt
    12541 ccacgcgtga aattcctggc ggagatggac tttggccgct tcgagatcac cgccatcgaa
    12601 gtaccatccg ttatagcgat tgagcgcgat gacgtcaatc agttcggcga ctttgtccgt
    12661 ctccggggta gccatcacaa acagcacgat cgtgaccgga cgcttctgtg ggtcgagttc
    12721 cttggtcagc tccaccaacg gcttgaagta ctcgtacgcg ccctcttcct cagtcgccgc
    12781 ctcgttggcg atgctccaca tcacgacgct tggatggttc ttgtcacgag acaccagttc
    12841 acggagaacg tcttgatggt gctcaaacgt ccgaatcttc tcccaggtac tgacgcgctc
    12901 gctgccttcg ccgagtcccg tggtggccat gaagttgagg tgcacgccaa ctgccggagt
    12961 ctcgtcgatc acgaccagac cctcgcgatc cgcaagacgc atcaactctt cagagtacgg
    13021 atagtgtgcg gtccggaagc tgttggcgcc gatccatttg aggatattga aatccatcac
    13081 attgctcgct tcgttaaagc cacggccgtt gataggagtg tcctcatgtt tgccaaagcc
    13141 cttgaagtag aacggtttgt tgttgatgag gaacttgccg tcgttgactt ccacggtccg
    13201 cacgccgaac ggctcttcat agacatcgat ggtcagtccg tcgttcacca gttccacttt
    13261 gatctggtag agatacgtgt tcagtggttc ccagaggatg acattcggaa tctccacgtt
    13321 accgctcagg ccctcggtgc ttgcgaccac tttgccttcc tcatccacga ccgacacttt
    13381 cacggtctcg gctttgcctt gaaagtccac cgtataggtc acagtcccgg ttgggccatt
    13441 gaagtcggtc acaaccgaga tgtcctcgac gtacgtaaac ggggtcgtgt agattttcac
    13501 cggacggtgc aggcctgcat agttgaagaa gtcgaagttc ggcttgttac gaatgacttt
    13561 tccgaggccc tcttcgtggc gctcgctgta cagccccacc gggagggtgc tatcgtcgag
    13621 gatgttgtcc acggcgacgg tgacgcgatt catgccatca cgcagcgagt tgttgatttc
    13681 cgcttcgaat ggcaggaatc cgcccttgtg ctccacgacc agctcaccat tgacatagac
    13741 aattgctttg tgagttgcag agccgaagcg gagcacgata cgctgatcct tcagataggc
    13801 cggcaccgtg aactcacgtt cgtaccagac atatccgata tggttgcgga tttccttggt
    13861 cacgccaatg tcattgtaac tgcttgggac ggccatacta atagtgtcgg tcagcttgct
    13921 ttcgtaccac ttctcttcca gtcctttccc gtagtccagc ttgaagttcc agacgccatt
    13981 gaggtcgaag acgccacggg tctcggtgtt gatcgggtac agactagttc gtcggttctg
    14041 taactatcat catcatcata gacacacgaa ataaagtaat cagattatca gttaaagcta
    14101 tgtaatattt acaccataac caatcaatta aaaaatagat cagtttaaag aaagatcaaa
    14161 gctcaaaaaa ataaaaagag aaaagggtcc taaccaagaa aatgaaggag aaaaactaga
    14221 aatttaccct cagatctacc atg
//
