LOCUS       sequence-477107-       11368 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  Expresses TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG 
            codon at position 1546 and a C-terminal HA tag in mammalian cells. 
            Can be used for click labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Stajkovic N, Liu Y, Arsic A, Meng N, Lyu H, Zhang N, Grimm D, Lerche
            H, Nikic-Spiegel I
  TITLE     Direct fluorescent labeling of NF186 and NaV1.6 in living primary 
            neurons using bioorthogonal click chemistry.
  JOURNAL   J Cell Sci. 2023 Jun 15;136(12):jcs260600. doi: 10.1242/jcs.260600. 
            Epub 2023 Jun 28.
  PUBMED    37288813
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2023-06-15"; volume: "136(12):jcs260600. doi"; pages: 
            " 10.1242/jcs.260600. Epub 2023 Jun 28"
COMMENT     Sequence Label: pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C)-HA
FEATURES             Location/Qualifiers
     source          1..11368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      958..967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2308..2317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6904..6930
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6959..6976)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6965..7189
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7235..7663
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7322..7341)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7532..7553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7672..7692)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7677..8006
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7857..7992
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7919..7938
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8073..8867
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8127..8146)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8737..8756
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9041..9162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9078..9097)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9132..9151
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9211..9227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9211..9227)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9224..9246)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9235..9251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9259..9265,9266..9283,9284..9289))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9304..9325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9442..9459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9613..10201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9693..9712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10372..11163,11164..11232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10995..11014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11233..11337)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc
      961 accatggctg cacggctgct ggcacctcct ggccccgatt cctttaagcc tttcacacct
     1021 gagagcctgg ctaacatcga gagaagaatt gccgagtcca agctgaagaa gcctcccaag
     1081 gccgatggct ctcacagaga ggacgacgag gattctaagc ctaagcccaa tagcgatctg
     1141 gaggccggca agtccctgcc tttcatctac ggcgacatcc cacagggcct ggtggccgtg
     1201 ccactggagg actttgaccc ctattacctg acccagaaga catttgtggt gctgaatcgg
     1261 ggcaagacac tgttccgctt ctctgccacc cccgccctgt atatcctgtc ccctttcaac
     1321 ctgatccgca gaatcgccat caagatcctg atccactccg tgttctctat gatcatcatg
     1381 tgcacaatcc tgaccaattg cgtgttcatg accttctcca atccaccaga gtggagcaag
     1441 aatgtggagt acaccttcac aggcatctac acctttgagt ctctggtgaa gatcatcgcc
     1501 cggggctttt gcatcgacgg cttcaccttt ctgagagacc catggaactg gctggacttt
     1561 agcgtgatca tgatggccta catcaccgag tttgtgaatc tgggcaacgt gagcgccctg
     1621 cggacattcc gggtgctgcg cgccctgaag accatctctg tgatccctgg cctgaagaca
     1681 atcgtgggcg ccctgatcca gtccgtgaag aagctgtccg acgtgatgat cctgaccgtg
     1741 ttctgcctgt ccgtgtttgc cctgatcggc ctgcagctgt tcatgggcaa cctgaggaac
     1801 aagtgcgtgg tgtggccaat caactttaat gagagctatc tggagaacgg caccagaggc
     1861 ttcgattggg aggagtacat caacaacaag accaatttct atatggtgcc tggcatgctg
     1921 gagccactgc tgtgcggcaa ctcctctgat gccggccagt gccccgaggg ctttcagtgc
     1981 atgaaggccg gcagaaatcc taattatggc tacacctctt ttgacacctt ttcctgggcc
     2041 tttctggccc tgttcagact gatgacccag gactgctggg agaacctgta ccagctgacc
     2101 ctgagagccg ccggcaagac ctacatgatc ttctttgtgc tggtaatctt cgtgggctcc
     2161 ttctacctgg tgaacctgat cctggccgtg gtggccatgg cctacgagga gcagaaccag
     2221 gccaccctgg aggaggccga gcagaaggag gccgagttca aggccatgct ggagcagctg
     2281 aagaagcagc aggaggaggc ccaggccgcc gccatggcca catccgccgg caccgtgagc
     2341 gaggacgcca tcgaggagga gggcgaggat ggcgtgggct ccccccggtc ttctagcgag
     2401 ctgtccaagc tgtcctctaa gtccgccaag gagaggcgga atcggagaaa gaagcggaag
     2461 cagaaggagc tgtccgaggg cgaggagaag ggcgatccag agaaggtgtt caagagcgag
     2521 tccgaggatg gcatgagacg gaaggccttt cgcctgcctg acaatagaat cggcaggaag
     2581 ttttccatca tgaatcagag cctgctgtcc atccctggca gccctttcct gtcccggcac
     2641 aattccaagt ccagcatctt ctcttttcgc ggccctggca gattccgcga tccaggctcc
     2701 gagaacgagt ttgccgatga cgagcacagc accgtggagg agtccgaggg ccggagagac
     2761 tccctgttta tccctatccg cgcccgggag cgcagatcct cttactccgg ctactccggc
     2821 tattctcagt gctccagatc ttctcggatc tttccatctc tgcggagatc cgtgaagaga
     2881 aactccaccg tggactgtaa tggcgtggtg agcctgatcg gccccggctc ccacatcggc
     2941 agactgctgc cagaggccac caccgaggtg gagatcaaga agaaggggcc tggcagcctg
     3001 ctggtgtcta tggagcagct ggccagctat ggcagaaagg accggatcaa ttctatcatg
     3061 tccgtggtga caaacaccct ggtggaggag ctggaggagt cccagaggaa gtgtccccca
     3121 tgttggtaca agtttgccaa tacatttctg atctgggagt gtcacccata ttggatcaag
     3181 ctgaaggaga tcgtgaatct gatcgtgatg gacccattcg tggatctggc catcacaatc
     3241 tgcatcgtgc tgaacaccct gtttatggcc atggagcacc accccatgac accacagttt
     3301 gagcacgtgc tggccgtggg caatctggtg ttcaccggca tctttaccgc cgagatgttc
     3361 ctgaagctga tcgccatgga cccatactac tattttcagg agggctggaa tatcttcgat
     3421 ggctttatcg tgagcctgtc cctgatggag ctgggcctgg ccgatgtgga gggcctgtct
     3481 gtgctgcgca gctttcggct gctgcgcgtg ttcaagctgg ccaagagctg gccaaccctg
     3541 aatatgctga tcaagatcat cggcaactcc gtgggcgccc tgggcaatct gaccctggtg
     3601 ctggccatca tcgtgttcat ctttgccgtg gtgggcatgc agctgttcgg caagtcctac
     3661 aaggagtgcg tgtgcaagat ctcccaggag tgtaagctgc cacggtggca catgaacgat
     3721 ttctttcact ctttcctgat cgtgttccgg gtgctgtgcg gcgagtggat cgagaccatg
     3781 tgggactgca tggaggtggc cggccaggcc atgtgcctga tcgtgttcat gatggtaatg
     3841 gtaatcggca atctggtggt gctgaatctg ttcctggccc tgctgctgtc ttcttttagc
     3901 gccgacaacc tggccgccac agacgatgac ggcgagatga ataacctgca gatctccgtg
     3961 atcagaatca agaagggcgt ggcctgggcc aaggtgaagg tgcacgcctt tatgcaggcc
     4021 cactttaagc agagggaggc cgatgaggtg aagccactgg acgagctgta cgagaagaag
     4081 gccaattgta tcgccaacca cacaggcgtg gatatacacc gcaatggcga tttccagaag
     4141 aacggcaatg gcaccacctc cggcatcggc tcctctgtgg agaagtacat catcgacgag
     4201 gaccacatgt ccttcatcaa taatccaaat ctgaccgtgc gggtgcctat cgccgtgggc
     4261 gagtctgact ttgagaatct gaacaccgag gacgtgtcca gcgagtccga cccagagggc
     4321 tccaaggaca agctggatga tacatcctct agcgagggct ctaccatcga catcaagcct
     4381 gaggtggagg aggtgcctgt ggagcagcca gaggagtacc tggaccctga tgcctgtttc
     4441 accgagggct gcgtgcagag attcaagtgc tgtcaggtga acatcgagga gggcctgggc
     4501 aagtcttggt ggatactgcg gaagacatgc ttcctgatcg tggagcacaa ctggtttgag
     4561 acctttatca tcttcatgat cctgctgtcc tccggcgccc tggcctttga ggacatctat
     4621 atcgagcaga gaaagaccat cagaaccatc ctggagtacg ccgacaaggt gttcacatat
     4681 atctttatcc tggagatgct gctgaagtgg accgcctacg gctttgtgaa gttctttacc
     4741 aatgcctggt gctggctgga ctttctgatc gtggccgtgt ctctggtgtc cctgatcgcc
     4801 aacgccctgg gctattctga gctgggcgcc atcaagtctc tgagaaccct gagagccctg
     4861 cgccccctga gagccctgag cagatttgag ggcatgaggg tggtggtgaa tgccctggtg
     4921 ggcgccatcc ctagcatcat gaacgtgctg ctggtgtgcc tgatcttttg gctgatcttt
     4981 tctatcatgg gcgtgaatct gttcgccggc aagtaccact actgctttaa tgagacctcc
     5041 gagatccggt tcgagatcga tgaggtgaac aacaagacag actgtgagaa gctgatggag
     5101 ggcaacaata cagagatccg gtggaagaat gtgaagatca atttcgataa cgtgggcgcc
     5161 ggctacctgg ccctgctgca ggtggccacc tttaagggct ggatggatat aatgtacgcc
     5221 gccgtggatt cccgcaagcc cgatgagcag ccagactatg agggcaacat ctacatgtac
     5281 atctatttcg tgatcttcat catcttcggc agctttttca ccctgaatct gtttatcggc
     5341 gtgatcatcg ataacttcaa ccagcagaag aagaagttcg gcggccagga catcttcatg
     5401 acagaggagc agaagaagta ctataacgcc atgaagaagc tgggctccaa gaagcctcag
     5461 aagccaatcc ctagaccact gaataagatc cagggcatcg tgttcgactt cgtgacccag
     5521 caggcctttg acatcgtgat catgatgctg atctgtctga acatggtgac catgatggtg
     5581 gagacagata cccagtccta gcagatggag aatatcctgt attggatcaa cctggtgttt
     5641 gtgatctttt tcacatgtga gtgcgtgctg aagatgttcg ccctgcgcca ctattatttc
     5701 acaatcggct ggaacatctt tgacttcgtg gtggtaatcc tgtctatcgt gggcatgttt
     5761 ctggccgaca tcatcgagaa gtacttcgtg agcccaacac tgttcagagt gatccggctg
     5821 gccaggatcg gcagaatcct gcggctgatc aagggcgcca agggcatcag aaccctgctg
     5881 ttcgccctga tgatgtctct gccagccctg ttcaatatcg gcctgctgct gtttctggta
     5941 atgttcatct tttccatctt tggcatgtcc aacttcgcct atgtgaagca cgaggccggc
     6001 atcgatgata tgtttaactt cgagacattc ggcaactcca tgatctgtct gttccagatc
     6061 accacctctg ccggctggga tggcctgctg ctgcctatcc tgaatagacc accagattgt
     6121 agcctggata aggagcaccc tggcagcggc ttcaagggcg attgtggcaa tccttccgtg
     6181 ggcatcttct tcttcgtgtc ctacatcatc atctctttcc tgatcgtggt gaacatgtac
     6241 atcgccatca tcctggagaa tttctctgtg gccaccgagg agtctgccga cccactgtct
     6301 gaggatgact tcgagacctt ctatgagatc tgggagaagt ttgaccctga tgccacccag
     6361 tttatcgagt attgcaagct ggccgacttt gccgacgccc tggagcaccc actgagagtg
     6421 ccaaagccaa ataccatcga gctgatcgcc atggatctgc ccatggtgag cggcgataga
     6481 atccactgcc tggacatcct gtttgccttc acaaagagag tgctgggcga tagcggcgag
     6541 ctggacatcc tgaggcagca gatggaggag aggttcgtgg cctctaaccc cagcaaggtg
     6601 tcctacgagc ctatcaccac aaccctgcgg agaaagcagg aggaggtgtc cgccgtggtg
     6661 ctgcagagag cctacagagg ccacctggcc cggagaggct ttatctgcag aaagatcacc
     6721 tccaacaagc tggagaatgg cggcacccac cgcgagaaga aggagtctac cccctctacc
     6781 gccagcctgc cttcctacga ctccgtgacc aagccagata aggagaagca gcagcgcgca
     6841 gaggagggac ggagagaaag agctaagaga cagaaagaag tgagagagtc aaagtgctct
     6901 agatacccat acgacgtccc agactacgct taagggcccg tttaaacccg ctgatcagcc
     6961 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7021 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     7081 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     7141 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     7201 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     7261 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7321 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7381 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7441 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7501 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7561 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7621 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     7681 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7741 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7801 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7861 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7921 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     7981 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     8041 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     8101 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     8161 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     8221 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     8281 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     8341 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     8401 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     8461 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     8521 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     8581 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     8641 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     8701 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     8761 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     8821 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     8881 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     8941 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     9001 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     9061 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9121 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     9181 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9241 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9301 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9361 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9421 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9481 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9781 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10021 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    10081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10681 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10981 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11341 atttccccga aaagtgccac ctgacgtc
//
