LOCUS       sequence-477110-       12121 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  Expresses independantly TTX-resistant (Y371C) variant of mouse 
            Nav1.6WT and eGFP in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12121)
  AUTHORS   Stajkovic N, Liu Y, Arsic A, Meng N, Lyu H, Zhang N, Grimm D, Lerche
            H, Nikic-Spiegel I
  TITLE     Direct fluorescent labeling of NF186 and NaV1.6 in living primary 
            neurons using bioorthogonal click chemistry.
  JOURNAL   J Cell Sci. 2023 Jun 15;136(12):jcs260600. doi: 10.1242/jcs.260600. 
            Epub 2023 Jun 28.
  PUBMED    37288813
REFERENCE   2  (bases 1 to 12121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2023-06-15"; volume: "136(12):jcs260600. doi"; pages: 
            " 10.1242/jcs.260600. Epub 2023 Jun 28"
COMMENT     Sequence Label: pcDNA3.1(+)_Nav1.6(WT, Y371C)-P2A-eGFP
FEATURES             Location/Qualifiers
     source          1..12121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      958..967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2308..2317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6913..6969
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6970..6972,6973..7686)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             6970..7683
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(7273..7292)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7620..7641
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7712..7729)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7718..7942
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7988..8416
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8075..8094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8285..8306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8425..8445)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8430..8759
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8610..8745
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8672..8691
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8826..9620
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8880..8899)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9490..9509
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9794..9915
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9831..9850)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9885..9904
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9964..9980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9964..9980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9977..9999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9988..10004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10012..10018,10019..10036,10037..10042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10057..10078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10195..10212)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10366..10954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10446..10465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11125..11916,11917..11985))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11748..11767
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11986..12090)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc
      961 accatggctg cacggctgct ggcacctcct ggccccgatt cctttaagcc tttcacacct
     1021 gagagcctgg ctaacatcga gagaagaatt gccgagtcca agctgaagaa gcctcccaag
     1081 gccgatggct ctcacagaga ggacgacgag gattctaagc ctaagcccaa tagcgatctg
     1141 gaggccggca agtccctgcc tttcatctac ggcgacatcc cacagggcct ggtggccgtg
     1201 ccactggagg actttgaccc ctattacctg acccagaaga catttgtggt gctgaatcgg
     1261 ggcaagacac tgttccgctt ctctgccacc cccgccctgt atatcctgtc ccctttcaac
     1321 ctgatccgca gaatcgccat caagatcctg atccactccg tgttctctat gatcatcatg
     1381 tgcacaatcc tgaccaattg cgtgttcatg accttctcca atccaccaga gtggagcaag
     1441 aatgtggagt acaccttcac aggcatctac acctttgagt ctctggtgaa gatcatcgcc
     1501 cggggctttt gcatcgacgg cttcaccttt ctgagagacc catggaactg gctggacttt
     1561 agcgtgatca tgatggccta catcaccgag tttgtgaatc tgggcaacgt gagcgccctg
     1621 cggacattcc gggtgctgcg cgccctgaag accatctctg tgatccctgg cctgaagaca
     1681 atcgtgggcg ccctgatcca gtccgtgaag aagctgtccg acgtgatgat cctgaccgtg
     1741 ttctgcctgt ccgtgtttgc cctgatcggc ctgcagctgt tcatgggcaa cctgaggaac
     1801 aagtgcgtgg tgtggccaat caactttaat gagagctatc tggagaacgg caccagaggc
     1861 ttcgattggg aggagtacat caacaacaag accaatttct atatggtgcc tggcatgctg
     1921 gagccactgc tgtgcggcaa ctcctctgat gccggccagt gccccgaggg ctttcagtgc
     1981 atgaaggccg gcagaaatcc taattatggc tacacctctt ttgacacctt ttcctgggcc
     2041 tttctggccc tgttcagact gatgacccag gactgctggg agaacctgta ccagctgacc
     2101 ctgagagccg ccggcaagac ctacatgatc ttctttgtgc tggtaatctt cgtgggctcc
     2161 ttctacctgg tgaacctgat cctggccgtg gtggccatgg cctacgagga gcagaaccag
     2221 gccaccctgg aggaggccga gcagaaggag gccgagttca aggccatgct ggagcagctg
     2281 aagaagcagc aggaggaggc ccaggccgcc gccatggcca catccgccgg caccgtgagc
     2341 gaggacgcca tcgaggagga gggcgaggat ggcgtgggct ccccccggtc ttctagcgag
     2401 ctgtccaagc tgtcctctaa gtccgccaag gagaggcgga atcggagaaa gaagcggaag
     2461 cagaaggagc tgtccgaggg cgaggagaag ggcgatccag agaaggtgtt caagagcgag
     2521 tccgaggatg gcatgagacg gaaggccttt cgcctgcctg acaatagaat cggcaggaag
     2581 ttttccatca tgaatcagag cctgctgtcc atccctggca gccctttcct gtcccggcac
     2641 aattccaagt ccagcatctt ctcttttcgc ggccctggca gattccgcga tccaggctcc
     2701 gagaacgagt ttgccgatga cgagcacagc accgtggagg agtccgaggg ccggagagac
     2761 tccctgttta tccctatccg cgcccgggag cgcagatcct cttactccgg ctactccggc
     2821 tattctcagt gctccagatc ttctcggatc tttccatctc tgcggagatc cgtgaagaga
     2881 aactccaccg tggactgtaa tggcgtggtg agcctgatcg gccccggctc ccacatcggc
     2941 agactgctgc cagaggccac caccgaggtg gagatcaaga agaaggggcc tggcagcctg
     3001 ctggtgtcta tggagcagct ggccagctat ggcagaaagg accggatcaa ttctatcatg
     3061 tccgtggtga caaacaccct ggtggaggag ctggaggagt cccagaggaa gtgtccccca
     3121 tgttggtaca agtttgccaa tacatttctg atctgggagt gtcacccata ttggatcaag
     3181 ctgaaggaga tcgtgaatct gatcgtgatg gacccattcg tggatctggc catcacaatc
     3241 tgcatcgtgc tgaacaccct gtttatggcc atggagcacc accccatgac accacagttt
     3301 gagcacgtgc tggccgtggg caatctggtg ttcaccggca tctttaccgc cgagatgttc
     3361 ctgaagctga tcgccatgga cccatactac tattttcagg agggctggaa tatcttcgat
     3421 ggctttatcg tgagcctgtc cctgatggag ctgggcctgg ccgatgtgga gggcctgtct
     3481 gtgctgcgca gctttcggct gctgcgcgtg ttcaagctgg ccaagagctg gccaaccctg
     3541 aatatgctga tcaagatcat cggcaactcc gtgggcgccc tgggcaatct gaccctggtg
     3601 ctggccatca tcgtgttcat ctttgccgtg gtgggcatgc agctgttcgg caagtcctac
     3661 aaggagtgcg tgtgcaagat ctcccaggag tgtaagctgc cacggtggca catgaacgat
     3721 ttctttcact ctttcctgat cgtgttccgg gtgctgtgcg gcgagtggat cgagaccatg
     3781 tgggactgca tggaggtggc cggccaggcc atgtgcctga tcgtgttcat gatggtaatg
     3841 gtaatcggca atctggtggt gctgaatctg ttcctggccc tgctgctgtc ttcttttagc
     3901 gccgacaacc tggccgccac agacgatgac ggcgagatga ataacctgca gatctccgtg
     3961 atcagaatca agaagggcgt ggcctgggcc aaggtgaagg tgcacgcctt tatgcaggcc
     4021 cactttaagc agagggaggc cgatgaggtg aagccactgg acgagctgta cgagaagaag
     4081 gccaattgta tcgccaacca cacaggcgtg gatatacacc gcaatggcga tttccagaag
     4141 aacggcaatg gcaccacctc cggcatcggc tcctctgtgg agaagtacat catcgacgag
     4201 gaccacatgt ccttcatcaa taatccaaat ctgaccgtgc gggtgcctat cgccgtgggc
     4261 gagtctgact ttgagaatct gaacaccgag gacgtgtcca gcgagtccga cccagagggc
     4321 tccaaggaca agctggatga tacatcctct agcgagggct ctaccatcga catcaagcct
     4381 gaggtggagg aggtgcctgt ggagcagcca gaggagtacc tggaccctga tgcctgtttc
     4441 accgagggct gcgtgcagag attcaagtgc tgtcaggtga acatcgagga gggcctgggc
     4501 aagtcttggt ggatactgcg gaagacatgc ttcctgatcg tggagcacaa ctggtttgag
     4561 acctttatca tcttcatgat cctgctgtcc tccggcgccc tggcctttga ggacatctat
     4621 atcgagcaga gaaagaccat cagaaccatc ctggagtacg ccgacaaggt gttcacatat
     4681 atctttatcc tggagatgct gctgaagtgg accgcctacg gctttgtgaa gttctttacc
     4741 aatgcctggt gctggctgga ctttctgatc gtggccgtgt ctctggtgtc cctgatcgcc
     4801 aacgccctgg gctattctga gctgggcgcc atcaagtctc tgagaaccct gagagccctg
     4861 cgccccctga gagccctgag cagatttgag ggcatgaggg tggtggtgaa tgccctggtg
     4921 ggcgccatcc ctagcatcat gaacgtgctg ctggtgtgcc tgatcttttg gctgatcttt
     4981 tctatcatgg gcgtgaatct gttcgccggc aagtaccact actgctttaa tgagacctcc
     5041 gagatccggt tcgagatcga tgaggtgaac aacaagacag actgtgagaa gctgatggag
     5101 ggcaacaata cagagatccg gtggaagaat gtgaagatca atttcgataa cgtgggcgcc
     5161 ggctacctgg ccctgctgca ggtggccacc tttaagggct ggatggatat aatgtacgcc
     5221 gccgtggatt cccgcaagcc cgatgagcag ccagactatg agggcaacat ctacatgtac
     5281 atctatttcg tgatcttcat catcttcggc agctttttca ccctgaatct gtttatcggc
     5341 gtgatcatcg ataacttcaa ccagcagaag aagaagttcg gcggccagga catcttcatg
     5401 acagaggagc agaagaagta ctataacgcc atgaagaagc tgggctccaa gaagcctcag
     5461 aagccaatcc ctagaccact gaataagatc cagggcatcg tgttcgactt cgtgacccag
     5521 caggcctttg acatcgtgat catgatgctg atctgtctga acatggtgac catgatggtg
     5581 gagacagata cccagtccaa gcagatggag aatatcctgt attggatcaa cctggtgttt
     5641 gtgatctttt tcacatgtga gtgcgtgctg aagatgttcg ccctgcgcca ctattatttc
     5701 acaatcggct ggaacatctt tgacttcgtg gtggtaatcc tgtctatcgt gggcatgttt
     5761 ctggccgaca tcatcgagaa gtacttcgtg agcccaacac tgttcagagt gatccggctg
     5821 gccaggatcg gcagaatcct gcggctgatc aagggcgcca agggcatcag aaccctgctg
     5881 ttcgccctga tgatgtctct gccagccctg ttcaatatcg gcctgctgct gtttctggta
     5941 atgttcatct tttccatctt tggcatgtcc aacttcgcct atgtgaagca cgaggccggc
     6001 atcgatgata tgtttaactt cgagacattc ggcaactcca tgatctgtct gttccagatc
     6061 accacctctg ccggctggga tggcctgctg ctgcctatcc tgaatagacc accagattgt
     6121 agcctggata aggagcaccc tggcagcggc ttcaagggcg attgtggcaa tccttccgtg
     6181 ggcatcttct tcttcgtgtc ctacatcatc atctctttcc tgatcgtggt gaacatgtac
     6241 atcgccatca tcctggagaa tttctctgtg gccaccgagg agtctgccga cccactgtct
     6301 gaggatgact tcgagacctt ctatgagatc tgggagaagt ttgaccctga tgccacccag
     6361 tttatcgagt attgcaagct ggccgacttt gccgacgccc tggagcaccc actgagagtg
     6421 ccaaagccaa ataccatcga gctgatcgcc atggatctgc ccatggtgag cggcgataga
     6481 atccactgcc tggacatcct gtttgccttc acaaagagag tgctgggcga tagcggcgag
     6541 ctggacatcc tgaggcagca gatggaggag aggttcgtgg cctctaaccc cagcaaggtg
     6601 tcctacgagc ctatcaccac aaccctgcgg agaaagcagg aggaggtgtc cgccgtggtg
     6661 ctgcagagag cctacagagg ccacctggcc cggagaggct ttatctgcag aaagatcacc
     6721 tccaacaagc tggagaatgg cggcacccac cgcgagaaga aggagtctac cccctctacc
     6781 gccagcctgc cttcctacga ctccgtgacc aagccagata aggagaagca gcagcgcgca
     6841 gaggagggac ggagagaaag agctaagaga cagaaagaag tgagagagtc aaagtgctct
     6901 agaggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac
     6961 cctggaccta tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     7021 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     7081 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     7141 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     7201 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     7261 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     7321 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     7381 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     7441 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     7501 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     7561 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     7621 atggtcctgc tggagttcgt gaccgccgcc gggatcactc acggcatgga cgagctgtac
     7681 aagtaagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     7741 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     7801 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     7861 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     7921 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     7981 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8041 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8101 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8161 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8221 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8281 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     8341 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8401 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     8461 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     8521 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8581 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8641 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     8701 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     8761 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     8821 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     8881 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     8941 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     9001 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     9061 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     9121 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     9181 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     9241 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     9301 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     9361 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     9421 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     9481 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     9541 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     9601 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     9661 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     9721 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     9781 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9841 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9901 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     9961 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    10021 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10081 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10141 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10201 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10261 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10321 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10381 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10441 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10501 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10561 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10621 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10681 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10741 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10801 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10861 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10921 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10981 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    11041 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    11101 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    11161 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    11221 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11281 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11341 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11401 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11461 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11521 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11581 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11641 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11701 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11761 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11821 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11881 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11941 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    12001 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    12061 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    12121 c
//
