LOCUS       sequence-477436-        8702 bp    DNA     circular SYN 01-JUL-2025
DEFINITION  Half of chemically inducible GFE3. When co-expressed with 236113 can
            degrade inhibitory synapses in presence of TH. (trimethoprim-HaloTag
            ligand)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Bareghamyan A, Deng C, Daoudi S, Yadav SC, Lu X, Zhang W, Campbell 
            RE, Kramer RH, Chenoweth DM, Arnold DB
  TITLE     A toolbox for ablating excitatory and inhibitory synapses.
  JOURNAL   eLife. 2025 Apr 29;13:RP103757. doi: 10.7554/eLife.103757.
  PUBMED    40298577
REFERENCE   2  (bases 1 to 8702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2025-04-29"; volume: "13:RP103757. doi"; pages: " 
            10.7554/eLife.103757"
COMMENT     Sequence Label: tagRFP-GPHN.FingR-HaloTag
FEATURES             Location/Qualifiers
     source          1..8702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    24..35
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     enhancer        39..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        420..695
                     /label=chicken beta-actin promoter
     intron          696..1712
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1636..1658
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1720..1739
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1773..1784
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             1791..2129
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQ"
     CDS             2136..2843
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             3672..4553
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     intron          4593..4725
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4708..4727
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4734..5322
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4787..4807)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5205..5216)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5349..5366)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5355..5466
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5562..5582)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5562..5580)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5601..5617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5723..5742)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5788..5843
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5842..5861)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6204..6220)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6228..6244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6252..6258,6259..6276,6277..6282))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6297..6318
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6377..6572
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6423..6558
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6578..6712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6615..6634)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6669..6688
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6780..6797)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6951..7539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7031..7050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7710..8501,8502..8570))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8333..8352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8571..8675)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agagtcatcc tcatcgtcga cattgattat tgactagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
      481 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc
      541 gcgcgccggg gggggggggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
      601 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
      661 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
      721 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
      781 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
      841 aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc
      901 ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg
      961 cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg
     1021 cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     1081 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     1141 tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg
     1201 gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg
     1261 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     1321 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     1381 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     1441 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     1501 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     1561 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     1621 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     1681 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     1741 tgtctcatca ttttggcaaa gaattcggta ccgtcatcct catcattatg ttccagtgcc
     1801 ggatctgcat gcggaacttc agcgaccggt ccaacctgag caggcacatc agaacccaca
     1861 ccggagaaaa gcccttcgcc tgcgacattt gcggccggaa gttcgccatc agcagcaacc
     1921 tgaacagcca caccaagatc cacactggca gccagaaacc tttccagtgc agaatttgta
     1981 tgagaaactt tagcagaagc gacaacctgg ccagacacat ccggacacat actggtgaaa
     2041 aaccttttgc ctgtgatatc tgtggcagaa agtttgccac ctccggcaat ctgacccggc
     2101 acacaaagat tcacctgcgg ggcagccagg gatccgtgtc taagggcgaa gagctgatta
     2161 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
     2221 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
     2281 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca
     2341 gcagaacctt catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg
     2401 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
     2461 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
     2521 tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc aacaccgaga
     2581 tgctgtaccc cgctgacggc ggcctggaag gcagaagcga catggccctg aagctcgtgg
     2641 gcgggggcca cctgatctgc aacttcaaga ccacatacag atccaagaaa cccgctaaga
     2701 acctcaagat gcccggcgtc tactatgtgg accacagact ggaaagaatc aaggaggccg
     2761 acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta
     2821 gcaaactggg gcacaaactt aatggtggag gttctggagg cggttcaggt ggaggttctg
     2881 gaggcggttc tccatggctc gaagtcaagg aagcatcacc aaccagcatc cagatcagct
     2941 ggggcaagta caaggtcatg gttcgctact accgcatcac ctacggtgaa actggtggca
     3001 atagccctgt ccaggaattc accgtgcctg gcagcaagtc cactgctacc atcagcagcc
     3061 tgaaacctgg tgtcgactat accatcacgg tgtacgccgt cacgatcgac cactggaact
     3121 accaggaccc gatcccgatc tccatcaact accgcaccgg ttcagctagc ggtggaggtt
     3181 ctggtggagg ttctggaggt ggatcaggag gaggttcgag atctaagctc tcaggtggcg
     3241 gagggagcgg cggaggtggc tctggaggtg gtggctcagc tgaagcatgg tacaatctgg
     3301 gaaacgctta ttacaagcag ggcgactatc aaaaagccat tgagtattac caaaaggctc
     3361 ttgagctaga ccctaataac gccgaagcgt ggtataacct aggcaacgca tattacaagc
     3421 agggagacta ccaaaaggca atcgaatatt accagaaggc actcgaactg gacccaaata
     3481 acgcagaggc ttggtataat ctgggaaacg cctactataa acagggtgac taccaaaagg
     3541 cgatagagga ctatcagaag gccttggagc tcgatccgaa caatctgcaa cgtagtgctg
     3601 gtggcggagg gtctggtgga ggaggctcag gaggaggtgg cgcatcaatc gatgcattaa
     3661 gcgccacgcg tgaaatcggt actggctttc cattcgaccc ccattatgtg gaagtcctgg
     3721 gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc
     3781 acggtaaccc gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc
     3841 atcgctgcat tgctccagac ctgatcggta tgggcaaatc cgacaaacca gacctgggtt
     3901 atttcttcga cgaccacgtc cgcttcatgg atgccttcat cgaagccctg ggtctggaag
     3961 aggtcgtcct ggtcattcac gactggggct ccgctctggg tttccactgg gccaagcgca
     4021 atccagagcg cgtcaaaggt attgcattta tggagttcat ccgccctatc ccgacctggg
     4081 acgaatggcc agaatttgcc cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc
     4141 gcaagctgat catcgatcag aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc
     4201 gcccgctgac tgaagtcgag atggaccatt accgcgagcc gttcctgaat cctgttgacc
     4261 gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg
     4321 tcgcgctggt cgaagaatac atggactggc tgcaccagtc ccctgtcccg aagctgctgt
     4381 tctggggcac cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc
     4441 tgcctaactg caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc
     4501 cggacctgat cggcagcgag atcgcgcgct ggctgtctac tctggagatt tcctaacaat
     4561 tgagtaaagc ggccccggac tcgaggccgc aggtaagtat caaggttaca agacaggttt
     4621 aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg
     4681 cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc gacaatcaac
     4741 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     4801 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     4861 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4921 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4981 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     5041 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     5101 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     5161 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     5221 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     5281 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt
     5341 acgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     5401 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5461 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     5521 caagggggag gattgggaag acaatagccc agcttttgtt ccctttagtg agggttaatt
     5581 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctaatt
     5641 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     5701 tcacaaatac cactgagatc gatctttttc cctctgccaa aaattatggg gacatcatga
     5761 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     5821 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     5881 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     5941 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     6001 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     6061 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     6121 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     6181 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6241 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6301 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6361 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6421 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6481 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6541 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     6601 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6661 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     6721 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6781 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6841 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6901 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6961 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7021 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7141 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7381 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7681 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7741 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7801 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7861 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7921 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7981 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8041 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8101 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8161 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8221 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8281 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8341 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8401 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8461 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8521 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8581 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8641 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8701 gg
//
