LOCUS       sequence-477509-       12103 bp    DNA     circular SYN 01-JUL-2025
DEFINITION  pLV-U6-[empty]-EFSNC-dSaCas9-DNMT3a-P2A-Puro-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12103)
  TITLE     SNCA-targeted epigenome therapy for Parkinson's disease alleviates 
            pathological and behavioral perturbations in a mouse model
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBK1579
FEATURES             Location/Qualifiers
     source          1..12103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        367..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     520..540
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             583..763
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    810..935
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1428..1661
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1846..1890
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2188..2305
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2382..2630
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2382..2402
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2553..2572
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2651..2726
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     promoter        2788..2999
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3007..3016
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3016..3081
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3091..3111
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3115..3135
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6370..7302
                     /codon_start=1
                     /product="catalytic domain of DNA methyltransferase 3A"
                     /label=DNMT3A catalytic domain
                     /translation="PSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATG
                     LLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVI
                     GGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVS
                     DKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGR
                     IAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSR
                     LARQRLLGRSWSVPVIRHLFAPLKEYFACV"
     CDS             7324..7380
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7381..7977
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7874..7894
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7993..8581
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8046..8066)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8464..8475)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8653..8886
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8912..8929)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8918..9142
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9188..9546
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9275..9294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9485..9506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9564..9583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9618..9637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9697..9713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9697..9713)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9710..9732)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9721..9737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9745..9751,9752..9769,9770..9775))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9790..9811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9928..9945)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10099..10687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10179..10198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10858..11649,11650..11718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11481..11500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11719..11823)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11898..11917)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(12089..12103,1..365)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
       61 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      121 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      181 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      241 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      301 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      361 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      421 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      481 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      541 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
      601 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      661 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      721 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      781 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      841 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      901 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      961 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     1021 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     1081 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1141 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1201 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1261 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1321 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1381 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1441 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1501 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1561 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1621 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1681 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1741 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1801 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1861 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1921 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1981 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2041 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     2101 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     2161 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2221 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2281 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     2341 gttaattaat gggcgggacg ttaacggggc ggaacggtac cgagggccta tttcccatga
     2401 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
     2461 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
     2521 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
     2581 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc ggagacgtgt
     2641 acacgtctct gttttagtac tctggaaaca gaatctacta aaacaaggca aaatgccgtg
     2701 tttatctcgt caacttgttg gcgagatttt ttgaattcgc tagctaggtc ttgaaaggag
     2761 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2821 agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact
     2881 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2941 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     3001 accggtgcca ccatggacta caaagaccat gacggtgatt ataaagatca tgacatagat
     3061 tacaaggatg acgatgacaa gggtggatcg ccaaagaaaa agaggaaagt cgccccaaag
     3121 aagaagcgga aggtcggtat ccacggagtc ccagcagcca agcggaacta catcctgggc
     3181 ctggccatcg gcatcaccag cgtgggctac ggcatcatcg actacgagac acgggacgtg
     3241 atcgatgccg gcgtgcggct gttcaaagag gccaacgtgg aaaacaacga gggcaggcgg
     3301 agcaagagag gcgccagaag gctgaagcgg cggaggcggc atagaatcca gagagtgaag
     3361 aagctgctgt tcgactacaa cctgctgacc gaccacagcg agctgagcgg catcaacccc
     3421 tacgaggcca gagtgaaggg cctgagccag aagctgagcg aggaagagtt ctctgccgcc
     3481 ctgctgcacc tggccaagag aagaggcgtg cacaacgtga acgaggtgga agaggacacc
     3541 ggcaacgagc tgtccaccaa agagcagatc agccggaaca gcaaggccct ggaagagaaa
     3601 tacgtggccg aactgcagct ggaacggctg aagaaagacg gcgaagtgcg gggcagcatc
     3661 aacagattca agaccagcga ctacgtgaaa gaagccaaac agctgctgaa ggtgcagaag
     3721 gcctaccacc agctggacca gagcttcatc gacacctaca tcgacctgct ggaaacccgg
     3781 cggacctact atgagggacc tggcgagggc agccccttcg gctggaagga catcaaagaa
     3841 tggtacgaga tgctgatggg ccactgcacc tacttccccg aggaactgcg gagcgtgaag
     3901 tacgcctaca acgccgacct gtacaacgcc ctgaacgacc tgaacaatct cgtgatcacc
     3961 agggacgaga acgagaagct ggaatattac gagaagttcc agatcatcga gaacgtgttc
     4021 aagcagaaga agaagcccac cctgaagcag atcgccaaag aaatcctcgt gaacgaagag
     4081 gatattaagg gctacagagt gaccagcacc ggcaagcccg agttcaccaa cctgaaggtg
     4141 taccacgaca tcaaggacat taccgcccgg aaagagatta ttgagaacgc cgagctgctg
     4201 gatcagattg ccaagatcct gaccatctac cagagcagcg aggacatcca ggaagaactg
     4261 accaatctga actccgagct gacccaggaa gagatcgagc agatctctaa tctgaagggc
     4321 tataccggca cccacaacct gagcctgaag gccatcaacc tgatcctgga cgagctgtgg
     4381 cacaccaacg acaaccagat cgctatcttc aaccggctga agctggtgcc caagaaggtg
     4441 gacctgtccc agcagaaaga gatccccacc accctggtgg acgacttcat cctgagcccc
     4501 gtcgtgaaga gaagcttcat ccagagcatc aaagtgatca acgccatcat caagaagtac
     4561 ggcctgccca acgacatcat tatcgagctg gcccgcgaga agaactccaa ggacgcccag
     4621 aaaatgatca acgagatgca gaagcggaac cggcagacca acgagcggat cgaggaaatc
     4681 atccggacca ccggcaaaga gaacgccaag tacctgatcg agaagatcaa gctgcacgac
     4741 atgcaggaag gcaagtgcct gtacagcctg gaagccatcc ctctggaaga tctgctgaac
     4801 aaccccttca actatgaggt ggaccacatc atccccagaa gcgtgtcctt cgacaacagc
     4861 ttcaacaaca aggtgctcgt gaagcaggaa gaagccagca agaagggcaa ccggacccca
     4921 ttccagtacc tgagcagcag cgacagcaag atcagctacg aaaccttcaa gaagcacatc
     4981 ctgaatctgg ccaagggcaa gggcagaatc agcaagacca agaaagagta tctgctggaa
     5041 gaacgggaca tcaacaggtt ctccgtgcag aaagacttca tcaaccggaa cctggtggat
     5101 accagatacg ccaccagagg cctgatgaac ctgctgcgga gctacttcag agtgaacaac
     5161 ctggacgtga aagtgaagtc catcaatggc ggcttcacca gctttctgcg gcggaagtgg
     5221 aagtttaaga aagagcggaa caaggggtac aagcaccacg ccgaggacgc cctgatcatt
     5281 gccaacgccg atttcatctt caaagagtgg aagaaactgg acaaggccaa aaaagtgatg
     5341 gaaaaccaga tgttcgagga aaagcaggcc gagagcatgc ccgagatcga aaccgagcag
     5401 gagtacaaag agatcttcat caccccccac cagatcaagc acattaagga cttcaaggac
     5461 tacaagtaca gccaccgggt ggacaagaag cctaatagag agctgattaa cgacaccctg
     5521 tactccaccc ggaaggacga caagggcaac accctgatcg tgaacaatct gaacggcctg
     5581 tacgacaagg acaatgacaa gctgaaaaag ctgatcaaca agagccccga aaagctgctg
     5641 atgtaccacc acgaccccca gacctaccag aaactgaagc tgattatgga acagtacggc
     5701 gacgagaaga atcccctgta caagtactac gaggaaaccg ggaactacct gaccaagtac
     5761 tccaaaaagg acaacggccc cgtgatcaag aagattaagt attacggcaa caaactgaac
     5821 gcccatctgg acatcaccga cgactacccc aacagcagaa acaaggtcgt gaagctgtcc
     5881 ctgaagccct acagattcga cgtgtacctg gacaatggcg tgtacaagtt cgtgaccgtg
     5941 aagaatctgg atgtgatcaa aaaagaaaac tactacgaag tgaatagcaa gtgctatgag
     6001 gaagctaaga agctgaagaa gatcagcaac caggccgagt ttatcgcctc cttctacaac
     6061 aacgatctga tcaagatcaa cggcgagctg tatagagtga tcggcgtgaa caacgacctg
     6121 ctgaaccgga tcgaagtgaa catgatcgac atcacctacc gcgagtacct ggaaaacatg
     6181 aacgacaaga ggccccccag gatcattaag acaatcgcct ccaagaccca gagcattaag
     6241 aagtacagca cagacattct gggcaacctg tatgaagtga aatctaagaa gcaccctcag
     6301 atcatcaaaa agggcaaaag gccggcggcc acgaaaaagg cgctcgaggg cggaggcggg
     6361 agcggatccc cctcccggct ccagatgttc ttcgctaata accacgacca ggaatttgac
     6421 cctccaaagg tttacccacc tgtcccagct gagaagagga agcccatccg ggtgctgtct
     6481 ctctttgatg gaatcgctac agggctcctg gtgctgaagg acttgggcat tcaggtggac
     6541 cgctacattg cctcggaggt gtgtgaggac tccatcacgg tgggcatggt gcggcaccag
     6601 gggaagatca tgtacgtcgg ggacgtccgc agcgtcacac agaagcatat ccaggagtgg
     6661 ggcccattcg atctggtgat tgggggcagt ccctgcaatg acctctccat cgtcaaccct
     6721 gctcgcaagg gcctctacga gggcactggc cggctcttct ttgagttcta ccgcctcctg
     6781 catgatgcgc ggcccaagga gggagatgat cgccccttct tctggctctt tgagaatgtg
     6841 gtggccatgg gcgttagtga caagagggac atctcgcgat ttctcgagtc caaccctgtg
     6901 atgattgatg ccaaagaagt gtcagctgca cacagggccc gctacttctg gggtaacctt
     6961 cccggtatga acaggccgtt ggcatccact gtgaatgata agctggagct gcaggagtgt
     7021 ctggagcatg gcaggatagc caagttcagc aaagtgagga ccattactac gaggtcaaac
     7081 tccataaagc agggcaaaga ccagcatttt cctgtgttca tgaatgagaa agaggacatc
     7141 ttatggtgca ctgaaatgga aagggtattt ggtttcccag tccactatac tgatgtgtcc
     7201 aacatgagcc gcttggcgag gcagagactg ctgggccggt catggagcgt gccagtcatc
     7261 cgccacctct tcgctccgct gaaggagtat tttgcgtgtg tgtccggccg gcccggatcc
     7321 ggcgcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa tcctggaccg
     7381 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     7441 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     7501 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     7561 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     7621 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     7681 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     7741 gcgtggttcc tggccaccgt cggagtctcg cccgaccacc agggcaaggg tctgggcagc
     7801 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7861 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7921 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg
     7981 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8041 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8101 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8161 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8221 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8281 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8341 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     8401 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8461 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8521 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     8581 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8641 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     8701 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8761 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8821 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8881 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     8941 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     9001 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     9061 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     9121 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     9181 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     9241 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     9301 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     9361 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     9421 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     9481 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     9541 gatttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9601 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     9661 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9721 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9781 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9841 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9901 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9961 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    10021 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    10081 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10141 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10201 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10261 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10321 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10381 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10441 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10501 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    10561 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10621 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10681 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10741 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10801 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10861 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10921 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10981 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    11041 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    11101 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11161 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11221 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11281 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11341 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11401 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11461 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11521 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11581 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11641 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11701 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11761 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11821 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
    11881 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
    11941 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
    12001 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
    12061 cgcgatgtac gggccagata tacgcgttga cattgattat tga
//
