LOCUS       sequence-477524-        9767 bp    DNA     circular SYN 01-JUL-2025
DEFINITION  Constitutive lentiviral expression of V5-mScarlet-FAM98B[1-330] 
            fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9767)
  TITLE     Neurodegenerative GGC repeat expansions disrupt tRNA processing 
            through aggregation of an endogenous glycine-rich sequence.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLJC5 mScarlet-FAM98B[1-330]
FEATURES             Location/Qualifiers
     source          1..9767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1824..3035
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2872..2891
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3056..3065
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3065..3106
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3113..3808
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3246..3264)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3514..3533
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4861..4978
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5027..5537
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5456..5474
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5559..6158
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5559..5578)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6055..6075
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6286..6519
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6591..6712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6628..6647)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6682..6701
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6752..6887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6907..6926)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6908..6926)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6936..6953)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6936..6952)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6945..6967)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7094..7549
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7181..7200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7391..7412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7575..7679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7680..7748,7749..8540)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7898..7917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8711..9299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9200..9219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9453..9470
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9587..9608
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9623..9628,9629..9646,9647..9653)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9661..9677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9666..9688
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9685..9701
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9685..9701
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9720..9740
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9722..9740
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     1861 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     1921 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     1981 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2041 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2101 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2161 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2221 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2281 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2341 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2401 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2461 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2521 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2581 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2641 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2701 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     2761 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     2821 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     2881 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     2941 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3001 ctaaattctg gccgtttttg gcttttttgt tagacgaagc gctagcgcta ccggtgccac
     3061 catgggtaag cctatcccta accctctcct cggtctcgat tctacgggct ctatggtgag
     3121 caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat
     3181 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     3241 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc
     3301 ccctcagttc atgtacggct ccagggcctt catcaagcac cccgccgaca tccccgacta
     3361 ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3421 cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga tctacaaggt
     3481 gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg
     3541 ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg gcgacattaa
     3601 gatggccctg cgcctgaagg acggcggccg ctacctggcg gacttcaaga ccacctacaa
     3661 ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtg gaccgcaagt tggacatcac
     3721 ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg gccgccactc
     3781 caccggcggc atggacgagc tgtacaaggg tggaggtggg tcgacgatga gagggccgga
     3841 gccgggtccc caaccgacga tggagggaga cgtgctggac acactggagg cgctggggta
     3901 taaaggacca ttgttagaag agcaagccct tacaaaggcg gcagagggtg gattatcttc
     3961 acctgaattt tcagagctct gtatttggtt aggctctcaa ataaaatcat tatgcaactt
     4021 ggaagaaagt atcacgtctg ctggaagaga tgatctagag agcttccagc ttgagataag
     4081 tggcttttta aaagaaatgg catgtccata ttctgtactc atatcaggag atattaaaga
     4141 tcgtttaaaa aagaaggagg actgtttgaa acttctatta tttttaagta cagaacttca
     4201 agcttcacag atattacaga acaagaaaca taaaaattct caattagata aaaatagtga
     4261 agtttatcag gaagttcaag ctatgtttga tacacttggt atacccaagt caacaacttc
     4321 tgacattccg catatgctaa accaagtgga atcaaaggtg aaagatattc tctcaaaggt
     4381 ccagaaaaat catgtgggaa aaccactgct gaaaatggat ttaaattcag aacaggcgga
     4441 acaactggaa agaatcaatg atgctctttc ctgtgaatat gagtgtcgcc gacgaatgtt
     4501 aatgaaacga ttagatgtga ctgtacagtc ctttggatgg tctgatagag caaaggtaaa
     4561 aacagatgat atagcaagaa tttatcagcc taagcgttat gctttgtcac ccaagacaac
     4621 gattacaatg gcacatctac ttgctgctcg tgaagatcta tccaagatca ttaggacaag
     4681 tagtggcacc agccgggaga agaccgcatg tgccattaat aaggtgctga tgggaagggt
     4741 gcctgacagg ggaggccggc cgaatgaaat tgaaccacca cctccagaaa tgcccccttg
     4801 gcaaaagaga caagaatgag aattctcgac ctcgagacaa atggcagtat tcatccacaa
     4861 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4921 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     4981 ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc
     5041 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     5101 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     5161 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     5221 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     5281 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     5341 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     5401 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     5461 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     5521 ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac aagcccacgg
     5581 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     5641 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     5701 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     5761 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     5821 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     5881 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     5941 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     6001 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     6061 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     6121 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
     6181 gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca atgacttaca
     6241 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6301 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6361 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6421 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6481 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6541 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     6601 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6661 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6721 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6781 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6841 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     6901 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     6961 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     7021 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     7081 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7141 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7201 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7261 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7321 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7381 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     7441 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     7501 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     7561 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7621 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7681 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7741 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7801 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7861 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7921 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7981 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8041 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8101 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8161 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8221 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8281 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8341 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8401 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8461 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8521 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8581 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8821 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9361 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     9421 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9481 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     9541 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     9601 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     9661 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     9721 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaa
//
