LOCUS       sequence-477583-         899 bp    DNA     linear   UNA 02-JUL-2025
DEFINITION  Expresses alpha synuclein (missing exon 3, residues 41-54) in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   SanGiovanni DQ, McGlinchey RP, Lee JC
  TITLE     Amyloid formation of alternatively spliced variants of 
            alpha-synuclein.
  JOURNAL   Protein Sci. 2025 Jul;34(7):e70195. doi: 10.1002/pro.70195.
  PUBMED    40522183
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2025-07"; volume: "34(7):e70195. doi"; pages: " 
            10.1002/pro.70195"
COMMENT     Sequence Label: pET21a asyn(Delta-41-54)
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             30..52
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             447..464
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(517..535)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      531..578
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(702..721)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gnngngnnan nnttnncctc tnnantnatt ttgtttaact ttaagaagga gatatacata
       61 tggatgtatt catgaaagga ctttcaaagg ccaaggaggg agttgtggct gctgctgaga
      121 aaaccaaaca gggtgtggca gaagcagcag gaaagacaaa agagggtgtt ctctatgtag
      181 tggctgagaa gaccaaagag caagtgacaa atgttggagg agcagtggtg acgggtgtga
      241 cagcagtagc ccagaagaca gtggagggag cagggagcat tgcagcagcc actggctttg
      301 tcaaaaagga ccagttgggc aagaatgaag aaggagcccc acaggaagga attctggaag
      361 atatgcctgt ggatcctgac aatgaggctt atgaaatgcc ttctgaggaa gggtatcaag
      421 actatgaacc tgaagcctaa ctcgagcacc accaccacca ccactgagat ccggctgcta
      481 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      541 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      601 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      661 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      721 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      781 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
      841 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtcca
//
