LOCUS       sequence-477584-         882 bp    DNA     linear   UNA 02-JUL-2025
DEFINITION  Expresses alpha synuclein (missing exon 5, residues 103-130) in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   SanGiovanni DQ, McGlinchey RP, Lee JC
  TITLE     Amyloid formation of alternatively spliced variants of 
            alpha-synuclein.
  JOURNAL   Protein Sci. 2025 Jul;34(7):e70195. doi: 10.1002/pro.70195.
  PUBMED    40522183
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2025-07"; volume: "34(7):e70195. doi"; pages: " 
            10.1002/pro.70195"
COMMENT     Sequence Label: pET21a asyn(Delta-103-130)
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             24..46
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             399..416
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(469..487)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      483..530
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(654..673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 annnntnnnc nnnctannat aattttgttt aactttaaga aggagatata catatggatg
       61 tattcatgaa aggactttca aaggccaagg agggagttgt ggctgctgct gagaaaacca
      121 aacagggtgt ggcagaagca gcaggaaaga caaaagaggg tgttctctat gtaggctcca
      181 aaaccaagga gggagtggtg catggtgtgg caacagtggc tgagaagacc aaagagcaag
      241 tgacaaatgt tggaggagca gtggtgacgg gtgtgacagc agtagcccag aagacagtgg
      301 agggagcagg gagcattgca gcagccactg gctttgtcaa aaaggaccag ttgggcaagg
      361 aagggtatca agactatgaa cctgaagcct aactcgagca ccaccaccac caccactgag
      421 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      481 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      541 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
      601 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      661 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      721 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      781 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
      841 gttggagtcc acgttcttta atagtggact cttgttccaa ac
//
