LOCUS       sequence-477587-       13264 bp    DNA     circular SYN 02-JUL-2025
DEFINITION  All-in-one plant virus expression vector for virus-assisted 
            transient expression (VATE)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13264)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 13264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVT
FEATURES             Location/Qualifiers
     source          1..13264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1225..1527)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1327..1346)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     promoter        complement(1601..1619)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1602..1619)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    1661..1677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1685..1691,1692..1709,1710..1715))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1730..1751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        complement(1904..2580)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(1947..1967)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(6350..6367)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6350..6366)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6359..6381)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6569..6593
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7894..8523
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8952..10025
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10091..10285
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10521..10543)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10629..10769
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10684..10703
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10784..10801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10955..11543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11035..11054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11630..12424)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12849..12873
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    13141..13162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13177..13182,13183..13200,13201..13207)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13215..13231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13239..13255
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcgagaccca cgctcacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     1201 ccattcgcca ttcaggcctg acatttatat tccccagaac atcaggttaa tggcgttttt
     1261 gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agacaggcac
     1321 actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta
     1381 aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg
     1441 tcgccccggc gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc
     1501 tcttttatag gtgtaaacct taaactgatg ttcgagcggt cgccagtgtg atgggtatct
     1561 acagactaga cccattgagc ttgatgcata gcttgagtat tctatagtgt cacctaaata
     1621 gcttggcgta atcatggtca tagctgtttc ttgtgtgaaa ttgttatccg ctcacaattc
     1681 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1741 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1801 agctgcatta atgaatcggc cacttcagag gtctcggatc tcattgcccc ccggatctgc
     1861 gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca
     1921 aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt
     1981 catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa
     2041 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
     2101 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac
     2161 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga
     2221 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg
     2281 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttc acatcaatcc
     2341 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
     2401 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
     2461 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
     2521 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
     2581 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
     2641 tgctccacca tgttggcaag ctgctctagc tcacgttgac ggaaactgca gaacgatctc
     2701 agttaggtca tgggaaagct gatattcatc cctctgagac tctatataat taaatgcgtt
     2761 tgggggatta gctaattgag aatccatata gagaaatatg gccgcgcagc gacgccggtt
     2821 gaagaatatg aacaaggaat aagatgaaca aggaagaaga tgaacaagca ataatttaat
     2881 taattattgt taataagcat aaattatgaa aataattttg atggtaatga aagtattata
     2941 aataatattt aggagaaaat attgttaagg aaatatctgg ttatgtttat ttgttaaggt
     3001 atatgggttt ttatagaaac ccaggagcag ctatggtaag aaacccagga ctcaaaaaat
     3061 gcctctcaac ttctctcata tgttgcggag tctggagtcc catttatacc atagaaagca
     3121 ttgggggtat agcatggtaa tttcacatcg gactccatac cgcggccatc tatataatat
     3181 tactgatggc cgcgcgaagt cccggtatgc cttttcagcc gttggatctg gagccgtacc
     3241 tttttcgcgt tcccattttg cccctggagt cccgagctat ttacgacccc ctgccaccga
     3301 gtccccttcg tggagcgctc ttcgtccaac acgtgtccca acttttgcgc atttttgact
     3361 acctgtcgcg cacttgaatt aaagttgccc ttttattttc ttccaattat tttgcggctg
     3421 agtagtctag tggatccccc ggagctccac ttgggatagg ttaagaaaat atgtctggct
     3481 tgtaaacgaa aagaatcggg gtttctaggc attttttaaa tagtgaccca ggactcaaaa
     3541 actagctctc aacttctctc atatgttgtg gagtctggag tcctttatat atgtttacgc
     3601 tatagaaggc atttggggac tctcccgggc ccaccatagg actccatacc gcggccatct
     3661 atataatatt actgatggcc gcgcggattt tggagccccc ttttgtcgtt agcgcgacct
     3721 ttaattcaaa ttaaaggttg cttttttcta tcgtccgatc attttgcgcc tgactagcct
     3781 agatattagc aacaacttcg cggctaagtt gttgaccgtc aagtataaat taatgggctt
     3841 atggcctaat gggtatagag caaaatggca tgcctgcaga ctagtgcttt actctgatcc
     3901 gcagatattt catcaggaaa gagctttgca agttccttca ttgtagcatc aataatttct
     3961 gagtcactac atgcaatcca ttcttctgct ggggcaaaaa ctaactccaa catggattgg
     4021 tttggattgt aatattcctt acatgttaca gacatgtcag cataaacact taaaagcggg
     4081 cttctgctaa agagtagatg atcataggtg ttcttcaatt tcctatcaaa ccagatgtga
     4141 acgttgataa ctggaactcc aactaatttc tctaatttct tgaagtatga aatctctctc
     4201 cagtcttcag gcactagtga attcgctagc gttaactggc cataatcctg tgtatgcaac
     4261 gttgaaaatt cggatctatt tttacgattc gatcatgaat taataaaatt taaattttat
     4321 tgaatgattt tctagtacat aactgacata cgacttatca gttgcgaaac gaacagctct
     4381 aattacattg ttaagtgaaa ttacgcctaa ttcgtctaag tacaacataa ccaactgttt
     4441 aaacctaatt aaataagtcg acccagaagc tgtcagagat gtcgtccaga cttggaagtt
     4501 caggtaagct ttgtggagat gcaacgctct ccgtaagttg tggttgaatc ggatctggac
     4561 gtggtatata cgcgtcctgg tgtatagcag atcctctact ccgtatatct tgaaatatag
     4621 gggatttatt atctcccaga tatacacgcc attctctgcc tgacgtgcag tgatgatttc
     4681 ccctgtgcgt gaatccatga ccggtgcagc ctatgtggaa gtatatggtg cacccacagt
     4741 ttagatcaat gcgtcgtcgt ctaatcgccc tcctcttggc ttgcctgtgt gccttcttga
     4801 tagagggggg cgtcgagagt gatgaagata acattcttga gagtccaatg tttgagcgac
     4861 gcattttccg ctttatccag gaaatcttta tagctggatc cctcgcctgg attgcaaagc
     4921 acgattgaag ggatccctcc tttaatttga actggtttcc cgtacttgca atttgactgc
     4981 cagtcttttt gggccccaag aagctctttc caatgcttta gctttaggta ttgcggtgct
     5041 acgtcatcaa tgacgttata ggcaacatta ttcgagtaca ctcgattatt gaagtccagg
     5101 tgtccactga ggtaattatg tgggcctaaa gcacgagccc acatcgtctt gcccgttcta
     5161 gaatcacctt ccactatgag actaactggt cttacaggcc gcgcagcggg acccattcca
     5221 aaataatcat ccgcccattc ttgcatctgt tcaggaacgt tagtgaaaga ggaaagtgga
     5281 aatggaggga cccattgttc aggcgccttt ttgaaaaggc gttccaagtt cgctgtaatg
     5341 ttatggtaac tcacgatgaa cgcccttgga tctccggccc ggataattgc aagagcttcc
     5401 gccaaactag gtgcattgat ggcgttataa tagacgtcgt ctttattctt ctttgtaccc
     5461 ccaggcactt tgaactgtcc ggattcacaa caatcgccgt ctttggtgat gtaattcttg
     5521 acggcgttgg cgtctttggc tgcttgtatg tttgggtgaa aattggtcga ccttctgggg
     5581 tgagtaaggt cgaaaaatct cttattcttg atgttggact ttccagagag ttggatgaga
     5641 cagtgaaggt gtgggaatcc gtccgagtgc tcctctctgg caactcgaat atatgttggt
     5701 ttgacgactg accatgataa ggtttgaagc atttgaagag cttcattttt tgggatatcg
     5761 cacttgggat aggttaagaa aatatgtctg gcttgtaaac gaaaagaatc ggggtttcta
     5821 ggcatttttt aaatagtgac ccaggactca aaaactagct ctcaacttct ctcatatgtt
     5881 gtggagtctg gagtccttta tatatgttta cgctatagaa ggcatttggg gactctcccg
     5941 ggcccaccat aggactccat accgcggcca tctatataat attactgatg gccgcgcgga
     6001 ttttggagcc cccttttgtc gttagcgcga cctttaattc aaattaaagg ttgctttttt
     6061 ctatcgtcca atcattttgc gcctgactag cctagatatt agcaacaact tcgcgactaa
     6121 gttgttgacc gtcaagtata aattaatggg cttatggccc aatgggtata gagcaaaatg
     6181 cccaagcgtg atgccccgtg gcgcttatta gcgggtacgt ctaaggtaag tcgaaatgcc
     6241 aactactctc ccttgagagt attgggccct aaagttaata agggacctcg agggggggcc
     6301 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca ctggccgtcg
     6361 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     6421 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     6481 agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt
     6541 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     6601 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     6661 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
     6721 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
     6781 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     6841 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     6901 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     6961 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     7021 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     7081 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     7141 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     7201 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     7261 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     7321 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     7381 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     7441 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     7501 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     7561 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     7621 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     7681 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     7741 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     7801 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     7861 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     7921 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     7981 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     8041 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     8101 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     8161 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     8221 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     8281 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     8341 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     8401 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     8461 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     8521 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     8581 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     8641 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     8701 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     8761 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     8821 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     8881 ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc
     8941 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     9001 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     9061 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     9121 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     9181 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     9241 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     9301 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     9361 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     9421 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     9481 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     9541 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     9601 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     9661 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     9721 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     9781 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     9841 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     9901 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     9961 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
    10021 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
    10081 gtgtctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
    10141 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
    10201 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
    10261 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
    10321 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
    10381 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
    10441 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
    10501 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
    10561 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    10621 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
    10681 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    10741 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
    10801 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    10861 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    10921 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    10981 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    11041 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    11101 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    11161 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    11221 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    11281 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    11341 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    11401 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    11461 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    11521 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    11581 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
    11641 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
    11701 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
    11761 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
    11821 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
    11881 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
    11941 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
    12001 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
    12061 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
    12121 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
    12181 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
    12241 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
    12301 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
    12361 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
    12421 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
    12481 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
    12541 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
    12601 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
    12661 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
    12721 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
    12781 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
    12841 ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac
    12901 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg atagcccttt
    12961 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
    13021 tgttggcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    13081 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    13141 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    13201 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
    13261 tacg
//
