LOCUS       sequence-477591-       13358 bp    DNA     circular SYN 02-JUL-2025
DEFINITION  This all-in-one vector is used to specifically knock out the GhCLA 
            gene via virus-induced genome editing (VIGE)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13358)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 13358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVE-GhCLA
FEATURES             Location/Qualifiers
     source          1..13358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        4230..4305
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(6444..6461)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6444..6460)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6453..6475)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6663..6687
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7988..8617
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9046..10119
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10185..10379
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10615..10637)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10723..10863
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10778..10797
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10878..10895)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11049..11637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11129..11148)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11724..12518)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12943..12967
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    13235..13256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13271..13276,13277..13294,13295..13301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13309..13325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13314..13336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13333..13349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13333..13349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtata ggaaaaaata tttattattt
      961 tttaaattta acctattgaa aatgtacagt ttaaagagta gacgtggatc gaattacatt
     1021 caagcacgtg gaaactcacg gaccccatta ttcaaacgac attccactgg taagcgttat
     1081 ggaggaagac gactacagcc actgaatgtg aaaagagaag atgaacccag aatgtcgacc
     1141 caacgcatcc acgagaatca atatgggtcg gaatttacaa tggcccacaa cacagccatt
     1201 tcgacctata tctcctatcc taatctgggc aagactgaac ccaatcgaac caggtcgtac
     1261 atcaagctga aacgattgcg tttcaagggc acggtgaaga tcgaacgagt tcctactgac
     1321 gtggctatgg atggttcgat tcccaacacg gaaggggtgt tctcattggt catagtcgtt
     1381 gaccgaaaac ctcatttgac tgcatctgga tgcctacata cgtttgacga actatttggt
     1441 gcgataatcc acagccatgg aaccctgtct atagttcccg cgttgaagga tcgttactac
     1501 atacggcatg taattaaacg ggtactgtcc gtcgagaaag ataacgtcat ggtggatatt
     1561 gaaggatcca ctgctctgtc taataggcgt tttaattgtt gggccagttt taaagatctt
     1621 gatttggatt catgtaaggg tgtttatgac aacataaaca agaatgccct gttagtttat
     1681 tactcatggg tatcggatac tgtgtctaaa gcatccactt atgtatcgta tgaccttgat
     1741 tatattggat aatcaataat aacaggaaaa tttgaataag aaaaaatatt atttaaatat
     1801 ttttggttga ggaccattac aactactctt gatacattct tggaccgttg tcctaaccag
     1861 ttcgtttaat tgggccatag acatggtaat gttggactca gccctatggg cccctactat
     1921 tgatgccgag tcacctggat ccagtacagt ggaccctaac ctgtgcagct ctctgtaagg
     1981 tgtctgttcg ttattggact gtgagtccgc atcttcgtta acgggcccta tggtactcct
     2041 ggtcgcccac gactcccctg gccttatttc tattgggcct ggaagcccaa atctgttcat
     2101 ggatgcggac ctaattagtt tcctttccca tttaccatag tctacatgtg agaaatcaac
     2161 gtctttagcg gagaactgtt tggaaaggat ctttaccgtt ggcgccttga acggaatatc
     2221 gacggaatgt ttggcggtcg ataatttcag cttccctttg aattttgcga agtgggtacg
     2281 ctgttgaaca ttcgtatcag aaactctgta atagagtttc caagggattg ggtctttgag
     2341 agagaagaag gaagctgaaa aatagtggag atctatgtta cacctgatcg gaaaagtcca
     2401 cgacgattgt aatgattcat tatcagtcat cctcttgtcg tgaatctcca ctatcactgt
     2461 accagtcgcg ttaattggca cctgctgcct gtactcgata acgcaatgat cgatcttcat
     2521 gcaactgcga ctgagtctcg ccgttaattg tgccgccgtt gacggaaact gcagaacgat
     2581 ctcagttagg tcatgggaaa gctgatattc atccctctga gactctatat aattaaatgc
     2641 gtttggggga ttagctaatt gagaatccat atagagaaat atggccgcgc agcgacgccg
     2701 gttgaagaat atgaacaagg aataagatga acaaggaaga agatgaacaa gcaataattt
     2761 aattaattat tgttaataag cataaattat gaaaataatt ttgatggtaa tgaaagtatt
     2821 ataaataata tttaggagaa aatattgtta aggaaatatc tggttatgtt tatttgttaa
     2881 ggtatatggg tttttataga aacccaggag cagctatggt aagaaaccca ggactcaaaa
     2941 aatgcctctc aacttctctc atatgttgcg gagtctggag tcccatttat accatagaaa
     3001 gcattggggg tatagcatgg taatttcaca tcggactcca taccgcggcc atctatataa
     3061 tattactgat ggccgcgcga agtcccggta tgccttttca gccgttggat ctggagccgt
     3121 acctttttcg cgttcccatt ttgcccctgg agtcccgagc tatttacgac cccctgccac
     3181 cgagtcccct tcgtggagcg ctcttcgtcc aacacgtgtc ccaacttttg cgcatttttg
     3241 actacctgtc gcgcacttga attaaagttg cccttttatt ttcttccaat tattttgcgg
     3301 ctgagtagtc tagtggatcc cccggagctc cacttgggat aggttaagaa aatatgtctg
     3361 gcttgtaaac gaaaagaatc ggggtttcta ggcatttttt aaatagtgac ccaggactca
     3421 aaaactagct ctcaacttct ctcatatgtt gtggagtctg gagtccttta tatatgttta
     3481 cgctatagaa ggcatttggg gactctcccg ggcccaccat aggactccat accgcggcca
     3541 tctatataat attactgatg gccgcgcgga ttttggagcc cccttttgtc gttagcgcga
     3601 cctttaattc aaattaaagg ttgctttttt ctatcgtccg atcattttgc gcctgactag
     3661 cctagatatt agcaacaact tcgcggctaa gttgttgacc gtcaagtata aattaatggg
     3721 cttatggcct aatgggtata gagcaaaatg gcatgcctgc agactagtgt tgaacaacgg
     3781 aaactcgact tgccttccgc acaatacatc atttcttctt agcttttttt cttcttcttc
     3841 gttcatacag tttttttttg tttatcagct tacattttct tgaaccgtag ctttcgtttt
     3901 cttcttttta actttccatt cggagttttt gtatcttgtt tcatagtttg tcccaggatt
     3961 agaatgatta ggcatcgaac cttcaagaat ttgattgaat aaaacatctt cattcttaag
     4021 atatgaagat aatcttcaaa aggcccctgg gaatctgaaa gaagagaagc aggcccattt
     4081 atatgggaaa gaacaatagt atttcttata taggcccatt taagttgaaa acaatcttca
     4141 aaagtcccac atcgcttaga taagaaaacg aagctgagtt tatatacagc tagagtcgaa
     4201 gtagtgattg gtgatggtgc tatgactgcg ttttagagct agaaatagca agttaaaata
     4261 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttttattcgc
     4321 tagcgttaac tggccataat cctgtgtatg caacgttgaa aattcggatc tatttttacg
     4381 attcgatcat gaattaataa aatttaaatt ttattgaatg attttctagt acataactga
     4441 catacgactt atcagttgcg aaacgaacag ctctaattac attgttaagt gaaattacgc
     4501 ctaattcgtc taagtacaac ataaccaact gtttaaacct aattaaataa gtcgacccag
     4561 aagctgtcag agatgtcgtc cagacttgga agttcaggta agctttgtgg agatgcaacg
     4621 ctctccgtaa gttgtggttg aatcggatct ggacgtggta tatacgcgtc ctggtgtata
     4681 gcagatcctc tactccgtat atcttgaaat ataggggatt tattatctcc cagatataca
     4741 cgccattctc tgcctgacgt gcagtgatga tttcccctgt gcgtgaatcc atgaccggtg
     4801 cagcctatgt ggaagtatat ggtgcaccca cagtttagat caatgcgtcg tcgtctaatc
     4861 gccctcctct tggcttgcct gtgtgccttc ttgatagagg ggggcgtcga gagtgatgaa
     4921 gataacattc ttgagagtcc aatgtttgag cgacgcattt tccgctttat ccaggaaatc
     4981 tttatagctg gatccctcgc ctggattgca aagcacgatt gaagggatcc ctcctttaat
     5041 ttgaactggt ttcccgtact tgcaatttga ctgccagtct ttttgggccc caagaagctc
     5101 tttccaatgc tttagcttta ggtattgcgg tgctacgtca tcaatgacgt tataggcaac
     5161 attattcgag tacactcgat tattgaagtc caggtgtcca ctgaggtaat tatgtgggcc
     5221 taaagcacga gcccacatcg tcttgcccgt tctagaatca ccttccacta tgagactaac
     5281 tggtcttaca ggccgcgcag cgggacccat tccaaaataa tcatccgccc attcttgcat
     5341 ctgttcagga acgttagtga aagaggaaag tggaaatgga gggacccatt gttcaggcgc
     5401 ctttttgaaa aggcgttcca agttcgctgt aatgttatgg taactcacga tgaacgccct
     5461 tggatctccg gcccggataa ttgcaagagc ttccgccaaa ctaggtgcat tgatggcgtt
     5521 ataatagacg tcgtctttat tcttctttgt acccccaggc actttgaact gtccggattc
     5581 acaacaatcg ccgtctttgg tgatgtaatt cttgacggcg ttggcgtctt tggctgcttg
     5641 tatgtttggg tgaaaattgg tcgaccttct ggggtgagta aggtcgaaaa atctcttatt
     5701 cttgatgttg gactttccag agagttggat gagacagtga aggtgtggga atccgtccga
     5761 gtgctcctct ctggcaactc gaatatatgt tggtttgacg actgaccatg ataaggtttg
     5821 aagcatttga agagcttcat tttttgggat atcgcacttg ggataggtta agaaaatatg
     5881 tctggcttgt aaacgaaaag aatcggggtt tctaggcatt ttttaaatag tgacccagga
     5941 ctcaaaaact agctctcaac ttctctcata tgttgtggag tctggagtcc tttatatatg
     6001 tttacgctat agaaggcatt tggggactct cccgggccca ccataggact ccataccgcg
     6061 gccatctata taatattact gatggccgcg cggattttgg agcccccttt tgtcgttagc
     6121 gcgaccttta attcaaatta aaggttgctt ttttctatcg tccaatcatt ttgcgcctga
     6181 ctagcctaga tattagcaac aacttcgcga ctaagttgtt gaccgtcaag tataaattaa
     6241 tgggcttatg gcccaatggg tatagagcaa aatgcccaag cgtgatgccc cgtggcgctt
     6301 attagcgggt acgtctaagg taagtcgaaa tgccaactac tctcccttga gagtattggg
     6361 ccctaaagtt aataagggac ctcgaggggg ggcccggtac ccggggatcc tctagagtcg
     6421 acctgcaggc atgcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6481 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6541 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc
     6601 tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg
     6661 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
     6721 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     6781 cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc
     6841 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt
     6901 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc
     6961 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc
     7021 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg
     7081 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg
     7141 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca
     7201 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
     7261 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
     7321 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
     7381 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
     7441 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
     7501 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
     7561 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
     7621 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
     7681 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
     7741 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc
     7801 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
     7861 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
     7921 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
     7981 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
     8041 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
     8101 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
     8161 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
     8221 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
     8281 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
     8341 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
     8401 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
     8461 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
     8521 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
     8581 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
     8641 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
     8701 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
     8761 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
     8821 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
     8881 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
     8941 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgcctg
     9001 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa
     9061 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
     9121 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
     9181 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
     9241 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
     9301 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
     9361 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
     9421 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
     9481 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
     9541 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
     9601 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
     9661 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
     9721 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
     9781 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
     9841 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
     9901 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
     9961 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
    10021 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
    10081 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
    10141 caaattgccc tagcagggga aaaaggtcga aaaggtgtct ttcctgtgga tagcacgtac
    10201 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
    10261 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
    10321 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
    10381 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
    10441 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
    10501 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
    10561 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
    10621 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10681 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
    10741 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
    10801 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    10861 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    10921 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    10981 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    11041 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    11101 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    11161 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    11221 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    11281 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    11341 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    11401 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    11461 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
    11521 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    11581 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    11641 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    11701 attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt
    11761 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
    11821 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
    11881 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
    11941 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
    12001 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
    12061 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
    12121 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
    12181 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
    12241 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
    12301 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
    12361 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc
    12421 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
    12481 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
    12541 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt
    12601 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt
    12661 tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa
    12721 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
    12781 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
    12841 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
    12901 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
    12961 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg
    13021 aattaacgcc gaattaattc ggggatagcc ctttggtctt ctgagactgt atctttgata
    13081 ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac
    13141 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    13201 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    13261 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    13321 aacaatttca cacaggaaac agctatgaca tgattacg
//
