LOCUS       sequence-477740-       10013 bp    DNA     circular SYN 03-JUL-2025
DEFINITION  Expression of recombinant protein for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Adriaenssens E, Schaar S, Cook ASI, Stuke JFM, Sawa-Makarska J, 
            Nguyen TN, Ren X, Schuschnig M, Romanov J, Khuu G, Lazarou M, Hummer
            G, Hurley JH, Martens S
  TITLE     Reconstitution of BNIP3/NIX-mediated autophagy reveals two pathways 
            and hierarchical flexibility of the initiation machinery.
  JOURNAL   bioRxiv [Preprint]. 2024 Aug 28:2024.08.28.609967. doi: 
            10.1101/2024.08.28.609967.
  PUBMED    39253418
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Aug 28:2024.08.28.609967. doi: 
            10.1101/2024.08.28.609967."
COMMENT     Sequence Label: pCAG-MBP-OSF-TEVcs-GFP-hULK1
FEATURES             Location/Qualifiers
     source          1..10013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1606..1628
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1690..1709
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1756..1765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1771..2871
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2845..2868
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2890..2913
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2950..2973
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2980..3003
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3010..3030
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             join(3037..3039,3040..3042,3043..3753)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3082..3103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3343..3362)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3690..3711
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7036..7055)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7101..7156
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7155..7174)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7516..7532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7516..7532)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7529..7551)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7540..7556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7564..7570,7571..7588,7589..7594))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7609..7630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7689..7884
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7735..7870
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7890..8024
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7927..7946)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7981..8000
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8092..8109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8263..8851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8343..8362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9022..9813,9814..9882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9645..9664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9883..9987)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggggg ggccaggggg gggggggggg gggggggggg
      541 gggggggggg gggggggggg gggggggggg ggggccccag gggggggggg gggggggggg
      601 cccccttttc gtgggggggg gggggggggg gggggcggta aaaaaagaga gccggcgggg
      661 gggggggggc ggcggggcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
      721 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
      781 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
      841 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
      901 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
      961 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     1021 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     1081 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     1141 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     1201 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
     1261 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
     1321 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     1381 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     1441 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1501 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     1561 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     1621 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     1681 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcctcg
     1741 atcgacggta tcgatgccac catggctagc atgaaaatcg aagaaggtaa actggtaatc
     1801 tggattaacg gcgataaagg ctataacggt ctcgctgaag tcggtaagaa attcgagaaa
     1861 gataccggaa ttaaagtcac cgttgagcat ccggataaac tggaagagaa attcccacag
     1921 gttgcggcaa ctggcgatgg ccctgacatt atcttctggg cacacgaccg ctttggtggc
     1981 tacgctcaat ctggcctgtt ggctgaaatc accccggaca aagcgttcca ggacaagctg
     2041 tatccgttta cctgggatgc cgtacgttac aacggcaagc tgattgctta cccgatcgct
     2101 gttgaagcgt tatcgctgat ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg
     2161 gaagagatcc cggcgctgga taaagaactg aaagcgaaag gtaagagcgc gctgatgttc
     2221 aacctgcaag aaccgtactt cacctggccg ctgattgctg ctgacggggg ttatgcgttc
     2281 aagtatgaaa acggcaagta cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa
     2341 gcgggtctga ccttcctggt tgacctgatt aaaaacaaac acatgaatgc agacaccgat
     2401 tactccatcg cagaagctgc ctttaataaa ggcgaaacag cgatgaccat caacggcccg
     2461 tgggcatggt ccaacatcga caccagcaaa gtgaattatg gtgtaacggt actgccgacc
     2521 ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc
     2581 agtccgaaca aagagctggc aaaagagttc ctcgaaaact atctgctgac tgatgaaggt
     2641 ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa
     2701 gagttggcga aagatccacg tattgccgcc actatggaaa acgcccagaa aggtgaaatc
     2761 atgccgaaca tcccgcagat gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac
     2821 gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac taattcgagc
     2881 tcggctagct ggagccaccc gcagttcgag aaaggtggag gtgcccgagg tggatcggga
     2941 ggtggatcgt ggagccaccc gcagttcgaa aaaggattcg attacaagga tgacgatgac
     3001 aagggtaccg aaaacctgta cttccaatcc accggtatgg tgagcaaggg cgaggagctg
     3061 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3121 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     3241 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     3361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     3481 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     3541 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3601 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     3661 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3721 gggatcactc tcggcatgga cgagctgtac aagaccggtg gtcccgggat ggaacctggc
     3781 agaggaggaa cagaaaccgt cggaaaattc gagtttagca gaaaggacct gattggacat
     3841 ggagcattcg cagtcgtgtt caagggtcgt caccgggaaa aacatgacct ggaggtggcc
     3901 gtcaagtgca tcaacaagaa aaatctggct aaatcccaga ccctgctcgg caaggaaatc
     3961 aaaattctga aggagctcaa acacgaaaac attgtggccc tgtacgactt ccaggaaatg
     4021 gctaactctg tgtacctggt catggagtat tgcaatggcg gagacctggc tgattatctc
     4081 catgcaatga gaaccctgag cgaggacact atccgtctgt tcctccagca gattgctggt
     4141 gcaatgcgtc tgctccactc caagggcatc attcatcggg atctgaaacc ccagaacatc
     4201 ctgctctcca atcctgctgg ccgcagggca aacccaaatt ctatcagggt gaagattgcc
     4261 gacttcggat ttgctagata cctgcagtct aacatgatgg ccgctacact gtgcggttca
     4321 cctatgtaca tggctccaga ggtcattatg tcacagcact atgacggaaa ggccgatctg
     4381 tggagtatcg gtactattgt gtaccagtgt ctgacaggca aagccccttt ccaggctagc
     4441 tccccacagg acctgcgcct cttttatgaa aagaacaaaa ccctggtgcc aactatcccc
     4501 cgtgagacct ctgcacctct gcgtcagctg ctcctggcac tcctgcagag aaatcacaag
     4561 gaccgtatgg acttcgatga gttctttcac catccctttc tggatgcttc acctagtgtg
     4621 aggaaatccc cccctgtgcc tgtcccaagc tacccatctt caggttccgg cagtagctcc
     4681 tcttcaagta gcacctctca cctggcatcc ccaccatctc tcggagagat gcagcagctg
     4741 cagaagaccc tcgcctcacc cgctgacact gcaggattcc tgcattcctc tagagattca
     4801 ggtggcagta aagactcaag ttgcgatact gacgatttcg tgatggtccc cgctcagttt
     4861 cctggagacc tggtcgccga agctcctagc gccaagcctc cacccgattc cctgatgtgt
     4921 tctggtagct ccctggtggc atctgcaggt ctcgagtcac acggtcgtac cccaagccct
     4981 tcccctccat gctcttcaag tccatctccc tcaggacggg caggtccatt cagctcctct
     5041 cgctgtggcg cctctgtgcc tatcccagtc cccacacagg tgcagaacta ccagcggatt
     5101 gaacgcaatc tgcagagccc aacacagttc cagacccccc gttcaagtgc tatcagacgt
     5161 agtggcagca cctccccact gggatttgct cgtgcatctc catcaccacc tgcacacgct
     5221 gagcatggag gtgtcctggc caggaagatg agtctcggcg gaggtagacc ctatacacct
     5281 agcccacaag tgggtaccat tcctgaaagg ccaggctggt caggaacccc cagtcctcag
     5341 ggtgctgaga tgaggggcgg aagatcccca cgtccaggta gctccgcacc agagcacagc
     5401 ccacgtactt ccggactggg ttgcaggctc cacagtgctc ctaacctgag cgacctccat
     5461 gtggtccgtc ccaagctgcc taaaccaccc accgatcccc tgggagcagt gttctcccct
     5521 ccacaggcat ctccacctca gccttcacat ggactgcaga gttgtcggaa cctccgcggt
     5581 agcccaaagc tgcccgactt cctccagaga aatcccctgc cacccatcct cggatccccc
     5641 acaaaggccg tcccttcttt cgattttcct aaaaccccat cttcacagaa cctcctggcc
     5701 ctcctggctc gtcagggtgt ggtcatgaca cctccacgca ataggaccct gccagacctc
     5761 tccgaagtgg gtcctttcca cggacagcca ctgggtcctg gactccgtcc aggcgaggat
     5821 ccaaagggtc ccttcggccg gagttttagc acttcccgcc tgacagacct cctgctcaaa
     5881 gcagccttcg gtacacaggc ccctgatcca ggcagcaccg agtctctgca ggagaagcca
     5941 atggagatcg caccctctgc cggctttggt ggttcactgc acccaggagc aagggcagga
     6001 ggtaccagta gcccctcacc tgtggtcttc actgtgggaa gtcccccttc tggttcaact
     6061 ccaccccagg gccctagaac acgtatgttt agtgctggac caactggtag cgcatcctct
     6121 tcagccagac acctggtccc aggaccctgc agcgaagctc ctgcaccaga gctgccagca
     6181 cctggtcatg gttgttcctt cgcagaccct atcactgcca acctggaagg cgctgtgaca
     6241 tttgaggcac ctgatctgcc agaggaaacc ctcatggagc aggaacacac tgagatcctg
     6301 aggggtctca gattcaccct gctctttgtg cagcatgtcc tggaaattgc tgcactcaag
     6361 ggtagtgcta gcgaggcagc tggtggacca gaataccagc tgcaggagag cgtggtcgcc
     6421 gaccagatct ctctgctctc aagggaatgg ggcttcgctg agcagctggt cctctatctg
     6481 aaggtggccg aactgctcag tagcggcctg cagtccgcca tcgatcagat tagagctgga
     6541 aagctctgcc tgtcctctac tgtgaaacag gtggtccggc gcctgaacga gctctacaag
     6601 gccagtgtgg tcagctgtca gggactctct ctgcgtctcc agcggttctt tctggacaaa
     6661 cagcgcctgc tcgataggat ccactcaatt acagctgaac gcctgatctt cagccatgca
     6721 gtgcagatgg tccagtccgc agccctggac gaaatgtttc agcacaggga gggatgcgtc
     6781 cccagatacc ataaggctct gctcctgctc gagggtctgc agcacatgct cagcgaccag
     6841 gccgatatcg aaaatgtgac taagtgcaaa ctgtgtattg agaggagact ctccgctctg
     6901 ctcacaggca tctgtgcata acccgggtga tatcttaatt aattaactcg aggaattcac
     6961 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     7021 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     7081 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     7141 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     7201 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     7261 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     7321 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     7381 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     7441 actactccca gtcatagctg tccctcttct cttatggaga tccccgacct gcagcccaag
     7501 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7561 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7621 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7681 gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7741 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7801 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7861 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     7921 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7981 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     8041 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8101 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8161 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8701 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8761 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8821 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8881 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8941 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9001 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9061 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9121 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9181 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9241 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9301 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9361 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9421 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9481 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9541 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9601 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9661 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9721 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9781 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9841 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9901 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9961 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
