LOCUS       sequence-477746-        8968 bp    DNA     circular SYN 03-JUL-2025
DEFINITION  shRNA-mediated knockdown of murine Fos.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8968)
  TITLE     Reza Kalhor Lab shRNA Plasmids 2025
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSGEP-shFos_2
FEATURES             Location/Qualifiers
     source          1..8968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     489..508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     742..759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    876..897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(912..917,918..935,936..942)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    950..966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     955..977
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     974..990
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     974..990
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1009..1029
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1011..1029
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1057..1283
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1284..1464
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1511..1636
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2129..2362
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2547..2591
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2858..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3013..3419
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(3442..3444,3445..3447,3448..4161)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3487..3508)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3748..3767)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4095..4116
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           4355..4369
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        4548..5047
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4583..4606)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4980..4999
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5068..5667
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5068..5087)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5564..5584
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5748..6336
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5801..5821)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6219..6230)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6543..6776
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6848..6969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6885..6904)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6939..6958
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7009..7144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7164..7183)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7165..7183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7193..7210)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7193..7209)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7202..7224)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7351..7806
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7438..7457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7648..7669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7832..7936
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7937..8005,8006..8797)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8155..8174)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8968,1..588)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
       61 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
      781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
      841 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      901 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
      961 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     1021 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     1081 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     1141 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     1201 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     1261 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     1321 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     1381 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     1441 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     1501 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     1561 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     1621 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1681 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1741 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1801 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1861 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1921 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1981 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     2041 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     2101 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     2161 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     2221 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     2281 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     2341 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     2401 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     2461 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     2521 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     2581 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     2641 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2701 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2761 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2821 ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg
     2881 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     2941 aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact agcctcgatc
     3001 gagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
     3061 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
     3121 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
     3181 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
     3241 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
     3301 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
     3361 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcggccggt
     3421 ggatctaccg gtggatccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     3481 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtctggcgag
     3541 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3601 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3661 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3721 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3781 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     3841 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     3901 atggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag
     3961 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4021 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4081 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4141 atggacgagc tgtacaagta atgacgtacg gcggccgcct cgactaggga taacagggta
     4201 attgtttgaa tgaggcttca gtactttaca gaatcgttgc ctgcacatct tggaaacact
     4261 tgctgggatt acttcgactt cttaacccaa cagaaggctc gagaaggtat attgctgttg
     4321 acagtgagcg ctgggttcat tattggaatt aatagtgaag ccacagatgt attaattcca
     4381 ataatgaacc caatgcctac tgcctcggac ttcaaggggc tagaattcga gcaattatct
     4441 tgtttactaa aactgaatac cttgctatct ctttgataca tttttacaaa gctgaattaa
     4501 aatggtataa attaaatcac ttttttcaat tgacgcgtaa ttctaccggg taggggaggc
     4561 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     4621 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     4681 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     4741 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     4801 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     4861 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     4921 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     4981 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     5041 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     5101 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     5161 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     5221 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     5281 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     5341 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     5401 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     5461 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     5521 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     5581 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     5641 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     5701 aggagcgcac gaccccatgc atgtcgactt cgaaacgcgt ctggaacaat caacctctgg
     5761 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5821 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5881 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5941 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6001 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6061 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6121 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     6181 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6241 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6301 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga
     6361 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     6421 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     6481 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6541 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6601 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6661 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6721 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     6781 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6841 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6901 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6961 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     7021 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7081 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7141 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     7201 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     7261 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     7321 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     7381 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     7441 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7501 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7561 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7621 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7681 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7741 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     7801 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7861 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7921 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7981 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     8041 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     8101 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     8161 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8221 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8281 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8341 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     8401 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8461 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8521 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8581 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8641 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8701 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8761 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8821 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8881 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8941 ccccgtagaa aagatcaaag gatcttct
//
