LOCUS       sequence-477747-       12479 bp    DNA     circular SYN 03-JUL-2025
DEFINITION  Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 
            isolate), featuring the E484D substitution, which likely emerged 
            during passaging and enhances TMEM106B binding..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12479)
  AUTHORS   Baggen J, Jacquemyn M, Persoons L, Vanstreels E, Pye VE, Wrobel AG, 
            Calvaresi V, Martin SR, Roustan C, Cronin NB, Reading E, Thibaut HJ,
            Vercruysse T, Maes P, De Smet F, Yee A, Nivitchanyong T, Roell M, 
            Franco-Hernandez N, Rhinn H, Mamchak AA, Ah Young-Chapon M, Brown E,
            Cherepanov P, Daelemans D
  TITLE     TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell 
            entry.
  JOURNAL   Cell. 2023 Aug 3;186(16):3427-3442.e22. doi: 
            10.1016/j.cell.2023.06.005. Epub 2023 Jul 7.
  PUBMED    37421949
REFERENCE   2  (bases 1 to 12479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.06.005"; journalName: "Cell"; date: "2023-08-3-
            3"; volume: "186"; issue: "16"; pages: "3427-3442.e22"
COMMENT     Sequence Label: 
            pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasti
            cidin
FEATURES             Location/Qualifiers
     source          1..12479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    join(4602..5176,5177..5179,5180..5188)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4769..4786)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4996..5015
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5208..5912
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      5460..5469
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5973..6029
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6030..6425
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6441..7029
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6494..6514)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6912..6923)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7101..7334
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7412..7533
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7449..7468)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7503..7522
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7573..7708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7728..7747)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7729..7747)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7757..7774)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7757..7773)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7766..7788)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7915..8370
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8002..8021)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8212..8233
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8396..8500
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8501..8569,8570..9361)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8719..8738)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9532..10120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10021..10040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10274..10291
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10408..10429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10444..10449,10450..10467,10468..10474)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10482..10498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10487..10509
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10506..10522
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10506..10522
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10541..10561
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10543..10561
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10589..10815
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10816..10996
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11043..11168
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11661..11894
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12079..12123
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12310..12427
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgggc ctcggcggcc aagcttggca atccggtact gttggtaaag
      841 ccaccatgtt cgtctttctg gtgctgctgc ccctggtctc ctcacagtgc gtcaatctga
      901 ctactcggac ccagctgccc cctgcctata ccaattcttt tacaaggggc gtgtactatc
      961 ctgataaggt gttccgcagc tccgtgctgc actccacaca ggacctgttt ctgccattct
     1021 tttctaatgt gacctggttc cacgccaagc ggtttgataa tccagtgctg ccctttaacg
     1081 acggcgtgta cttcgcctct accgagaaga gcaacatcat cagaggctgg atcttcggca
     1141 ccacactgga ttccaagaca cagtctctgc tgatcgtgaa caatgccacc aacgtggtca
     1201 tcaaggtgtg cgagttccag ttttgtaacg acccttttct gggcgtgtac tatcacaaga
     1261 acaataagag ctggatggag tccgagttca gagtgtattc tagcgccaac aattgcacat
     1321 ttgagtacgt gagccagcca ttcctgatgg atctggaggg caagcagggc aacttcaaga
     1381 acctgaggga gttcgtgttt aagaatatcg acggctactt caaaatctac agcaagcaca
     1441 cccccatcaa cctggtgcgc gatctgcctc agggcttttc cgccctggag cctctggtgg
     1501 acctgccaat cggcatcaac atcaccaggt tccagacact gctggccctg caccgctcct
     1561 acctgacacc aggcgattcc tctagcggat ggaccgcagg agctgccgcc tactatgtgg
     1621 gctatctgca gcctaggacc tttctgctga agtacaacga gaatggcacc atcacagacg
     1681 ccgtggattg cgccctggac ccactgtccg agacaaagtg tacactgaag tctttcaccg
     1741 tggagaaggg catctatcag acatctaatt ttcgggtgca gcccaccgag agcatcgtga
     1801 gattccctaa tatcacaaac ctgtgcccat ttggcgaggt gttcaacgca accaggttcg
     1861 ccagcgtgta cgcatggaat cggaagagaa tcagcaactg cgtggccgat tattccgtgc
     1921 tgtacaactc cgcctctttc agcaccttta agtgctatgg cgtgtctccc acaaagctga
     1981 atgacctgtg ctttaccaac gtgtacgccg atagcttcgt gatcaggggc gacgaggtga
     2041 gacagatcgc accaggacag acaggcaaga tcgcagatta caattataag ctgcctgacg
     2101 atttcaccgg ctgcgtgatc gcctggaact ctaacaatct ggacagcaaa gtgggcggca
     2161 actacaatta tctgtacagg ctgtttcgca agagcaatct gaagccattc gagcgggata
     2221 tcagcacaga aatctaccag gcaggctcca ccccatgcaa tggagtggat ggctttaact
     2281 gttatttccc actgcagtcc tacggctttc agcccaccaa cggcgtgggc tatcagcctt
     2341 acagagtggt ggtgctgtcc ttcgagctgc tgcacgcacc agcaacagtg tgcggaccta
     2401 agaagtctac caatctggtg aagaacaagt gcgtgaactt caacttcaac ggcctgaccg
     2461 gaacaggcgt gctgaccgag agcaataaga agttcctgcc ctttcagcag ttcggccggg
     2521 acatcgccga taccacagac gccgtgagag atccacagac cctggagatc ctggacatca
     2581 caccctgctc cttcggcggc gtgtctgtga tcacacctgg caccaataca tccaaccagg
     2641 tggccgtgct gtatcaggat gtgaattgta ccgaggtgcc tgtggcaatc cacgcagacc
     2701 agctgacccc aacatggagg gtgtacagca ccggctccaa cgtgtttcag acaagggcag
     2761 gatgtctgat cggagcagag cacgtgaaca atagctatga gtgcgacatc cccatcggcg
     2821 ccggcatctg tgcctcttac cagccacgga gagcaagaag tgtcgcctca cagtccatca
     2881 tcgcctatac aatgtccctg ggggcagaaa actcagtcgc atacagcaat aattctatcg
     2941 ccatccccac caactttaca atcagcgtga ccacagagat cctgcccgtg agcatgacca
     3001 agacatctgt ggattgcacc atgtacatct gtggcgacag cacagagtgc tccaatctgc
     3061 tgctgcagta tggctccttc tgtacccagc tgaacagggc cctgacagga atcgcagtgg
     3121 agcaggacaa gaatacccag gaggtgtttg cccaggtgaa gcaaatctac aagacacccc
     3181 ctatcaagga tttcggcggc ttcaactttt ctcagatcct gcctgaccca agcaagccca
     3241 tcaagaggtc tttcatcgag gatctgctgt ttaataaggt gaccctggcc gacgccggct
     3301 ttatcaagca gtacggcgac tgcctgggcg atatcgcagc acgcgatctg atctgtgccc
     3361 agaagttcaa cggcctgacc gtgctgccac cactgctgac agacgagatg atcgcacagt
     3421 ataccagcgc cctgctggcc ggaaccatca catccggatg gacatttggc gcaggagccg
     3481 ccctgcagat ccctttcgcc atgcagatgg cctaccgctt taatggcatc ggcgtgaccc
     3541 agaacgtgct gtatgagaat cagaagctga tcgccaacca gttcaattcc gccatcggca
     3601 agatccagga ttctctgagc tccaccgcca gcgccctggg caagctgcag gacgtggtga
     3661 accagaatgc ccaggccctg aacacactgg tgaagcagct gtctagcaat tttggcgcca
     3721 tctcctctgt gctgaacgac atcctgagcc ggctggataa ggtggaggcc gaggtgcaga
     3781 tcgatcggct gatcaccggc agactgcaga gcctgcagac ctacgtgaca cagcagctga
     3841 tcagggcagc agagatcagg gcctccgcca acctggccgc cacaaagatg tccgagtgcg
     3901 tgctgggcca gtctaagaga gtggatttct gtggcaaggg ctaccacctg atgtcttttc
     3961 cacagagcgc cccacacgga gtggtgttcc tgcacgtgac ctatgtgcct gcccaggaga
     4021 agaacttcac cacagcacca gcaatctgcc acgacggcaa ggcacacttc ccaagggagg
     4081 gcgtgttcgt gagcaatggc acccactggt tcgtgacaca gagaaacttt tatgagcccc
     4141 agatcatcac cacagataac accttcgtga gcggcaattg tgacgtggtc atcggcatcg
     4201 tgaacaatac agtgtacgac cccctgcagc ctgagctgga ttccttcaag gaggagctgg
     4261 acaagtactt caagaaccac acctctcctg acgtggatct gggcgacatc agcggcatca
     4321 acgcctccgt ggtgaatatc cagaaggaga tcgatcggct gaatgaggtg gccaagaacc
     4381 tgaatgagtc cctgatcgac ctgcaggagc tgggcaagta cgagcagtat atcaagtggc
     4441 catggtacat ctggctgggc ttcatcgccg gcctgatcgc catcgtgatg gtgacaatca
     4501 tgctgtgctg tatgaccagt tgctgttctt gcctgaaggg gtgttgttct tgtggaagtt
     4561 gttgctgagg taccggatcc ggaattccgc cccccccccc ccccctctcc ctcccccccc
     4621 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     4681 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     4741 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     4801 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     4861 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     4921 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     4981 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     5041 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     5101 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     5161 aaaacacgat gataagcttg ccacaaccca caaggagacg accttccatg gtgagcaagg
     5221 gcgaggagga taacgcctct ctcccagcga cacatgagtt acacatcttt ggctccatca
     5281 acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa tccaaatgat ggttatgagg
     5341 agttaaacct gaagtccacc aagggtgacc tccagttctc cccctggatt ctggtccctc
     5401 atatcgggta tggcttccat cagtacctgc cctaccctga cgggatgtcg cctttccagg
     5461 ccgccatggt agatggcagc ggataccaag tccatcgcac aatgcagttt gaagatggtg
     5521 cctcccttac tgttaactac cgctacacct acgagggaag ccacatcaaa ggagaggccc
     5581 aggtgaaggg gactggtttc cctgctgacg gtcctgtgat gaccaactcg ctgaccgctg
     5641 cggactggtg caggtcgaag aagacttacc ccaacgacaa aaccatcatc agtaccttta
     5701 agtggagtta caccactgga aatggcaagc gctaccggag cactgcgcgg accacctaca
     5761 cctttgccaa gccaatggcg gctaactatc tgaagaacca gccgatgtac gtgttccgta
     5821 agacggagct caagcactcc aagaccgagc tcaacttcaa ggagtggcaa aaggccttta
     5881 ccgatgtgat gggcatggac gagctgtaca agggatccaa gctttccaac gaactggccc
     5941 tgaagctggc tggtttggac atcggcagcg gagcaaccaa ttttagtctc ctcaaacagg
     6001 ccggcgatgt cgaagaaaat ccgggcccag ccaagccttt gtctcaagaa gaatccaccc
     6061 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     6121 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     6181 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     6241 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     6301 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     6361 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     6421 gctgaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6481 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6541 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6601 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6661 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6721 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6781 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6841 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6901 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6961 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7021 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7081 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     7141 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7201 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7261 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7321 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     7381 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7441 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7501 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7561 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7621 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7681 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     7741 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7801 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     7861 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     7921 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7981 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8041 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8101 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8161 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8221 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8281 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8341 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     8401 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8461 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8521 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8581 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8641 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8701 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8761 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8821 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8881 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8941 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9001 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9061 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9121 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9181 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9241 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9301 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9361 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9421 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9661 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     9961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10141 tttacggttc ctggtctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    10201 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    10261 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    10321 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    10381 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    10441 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    10501 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
    10561 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
    10621 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
    10681 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
    10741 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
    10801 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
    10861 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
    10921 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
    10981 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
    11041 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
    11101 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
    11161 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
    11221 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
    11281 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
    11341 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
    11401 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
    11461 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
    11521 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
    11581 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    11641 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    11701 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    11761 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    11821 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    11881 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    11941 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    12001 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    12061 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    12121 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    12181 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    12241 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccaga
    12301 gagggcctat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
    12361 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
    12421 aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc tcgaggggg
//
