LOCUS       sequence-477775-        9868 bp    DNA     circular SYN 03-JUL-2025
DEFINITION  Encodes full length human TMPRSS2 protein, along with hygromycin 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Baggen J, Jacquemyn M, Persoons L, Vanstreels E, Pye VE, Wrobel AG, 
            Calvaresi V, Martin SR, Roustan C, Cronin NB, Reading E, Thibaut HJ,
            Vercruysse T, Maes P, De Smet F, Yee A, Nivitchanyong T, Roell M, 
            Franco-Hernandez N, Rhinn H, Mamchak AA, Ah Young-Chapon M, Brown E,
            Cherepanov P, Daelemans D
  TITLE     TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell 
            entry.
  JOURNAL   Cell. 2023 Aug 3;186(16):3427-3442.e22. doi: 
            10.1016/j.cell.2023.06.005. Epub 2023 Jul 7.
  PUBMED    37421949
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.06.005"; journalName: "Cell"; date: "2023-08-3-
            3"; volume: "186"; issue: "16"; pages: "3427-3442.e22"
COMMENT     Sequence Label: pLCKO-CMV-TMPRSS2-IRES-Hygromycin
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2298..2760
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2354..2371)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2581..2600
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2793..3818
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2793..3818
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    3834..4422
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3887..3907)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4305..4316)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4494..4727
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4805..4926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4842..4861)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4896..4915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4966..5101
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5122..5140)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5150..5167)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5150..5166)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5159..5181)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5308..5763
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5395..5414)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5605..5626
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5789..5893
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5894..5962,5963..6754)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6112..6131)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6925..7513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7414..7433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7667..7684
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7801..7822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7837..7842,7843..7860,7861..7867)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7875..7891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7880..7902
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7899..7915
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7899..7915
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7934..7954
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7936..7954
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7982..8208
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8209..8389
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8436..8561
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9054..9287
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9472..9516
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9703..9820
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacgggccc tctagaccat ggctttgaac tcagggtcac caccagctat
      721 tggaccttac tatgaaaacc atggatacca accggaaaac ccctatcccg cacagcccac
      781 tgtggtcccc actgtctacg aggtgcatcc ggctcagtac tacccgtccc ccgtgcccca
      841 gtacgccccg agggtcctga cgcaggcttc caaccccgtc gtctgcacgc agcccaaatc
      901 cccatccggg acagtgtgca cctcaaagac taagaaagca ctgtgcatca ccttgaccct
      961 ggggaccttc ctcgtgggag ctgcgctggc cgctggccta ctctggaagt tcatgggcag
     1021 caagtgctcc aactctggga tagagtgcga ctcctcaggt acctgcatca acccctctaa
     1081 ctggtgtgat ggcgtgtcac actgccccgg cggggaggac gagaatcggt gtgttcgcct
     1141 ctacggacca aacttcatcc ttcaggtgta ctcatctcag aggaagtcct ggcaccctgt
     1201 gtgccaagac gactggaacg agaactacgg gcgggcggcc tgcagggaca tgggctataa
     1261 gaataatttt tactctagcc aaggaatagt ggatgacagc ggatccacca gctttatgaa
     1321 actgaacaca agtgccggca atgtcgatat ctataaaaaa ctgtaccaca gtgatgcctg
     1381 ttcttcaaaa gcagtggttt ctttacgctg tatagcctgc ggggtcaact tgaactcaag
     1441 ccgccagagc aggattgtgg gcggcgagag cgcgctcccg ggggcctggc cctggcaggt
     1501 cagcctgcac gtccagaacg tccacgtgtg cggaggctcc atcatcaccc ccgagtggat
     1561 cgtgacagcc gcccactgcg tggaaaaacc tcttaacaat ccatggcatt ggacggcatt
     1621 tgcggggatt ttgagacaat ctttcatgtt ctatggagcc ggataccaag tagaaaaagt
     1681 gatttctcat ccaaattatg actccaagac caagaacaat gacattgcgc tgatgaagct
     1741 gcagaagcct ctgactttca acgacctagt gaaaccagtg tgtctgccca acccaggcat
     1801 gatgctgcag ccagaacagc tctgctggat ttccgggtgg ggggccaccg aggagaaagg
     1861 gaagacctca gaagtgctga acgctgccaa ggtgcttctc attgagacac agagatgcaa
     1921 cagcagatat gtctatgaca acctgatcac accagccatg atctgtgccg gcttcctgca
     1981 ggggaacgtc gattcttgcc agggtgacag tggagggcct ctggtcactt cgaagaacaa
     2041 tatctggtgg ctgatagggg atacaagctg gggttctggc tgtgccaaag cttacagacc
     2101 aggagtgtac gggaatgtga tggtattcac ggactggatt tatcgacaaa tgagggcaga
     2161 cggctaaggt accggatccg gaattccccc cccccccccc ccccccctaa cgttactggc
     2221 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     2281 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     2341 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     2401 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     2461 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     2521 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     2581 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     2641 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     2701 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     2761 gcttgccaca acccacaagg agacgacctt ccatgaaaaa gcctgaactc accgcgacgt
     2821 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     2881 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     2941 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     3001 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     3061 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     3121 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     3181 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     3241 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     3301 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     3361 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     3421 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     3481 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     3541 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     3601 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     3661 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     3721 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     3781 accgacgccc cagcactcgt ccgagggcaa aggaatgaac gcgttaagtc gacaatcaac
     3841 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3901 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3961 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4021 ttgtcaggca acgcggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4081 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4141 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4201 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4261 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4321 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4381 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
     4441 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     4501 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     4561 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     4621 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     4681 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag
     4741 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     4801 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4921 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     4981 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5041 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5101 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     5161 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     5221 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     5281 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     5341 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5401 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5461 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5521 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5581 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5641 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5701 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5761 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5821 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5881 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5941 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6001 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6061 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6121 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6181 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6241 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6301 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6361 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6421 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6481 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6541 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6601 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6661 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6721 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6781 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6841 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6901 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6961 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7021 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7081 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7141 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7201 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7261 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7321 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7381 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7441 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7501 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggtct tttgctggcc
     7561 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7621 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7681 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7741 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7801 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7861 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7921 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     7981 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     8041 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     8101 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     8161 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     8221 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8281 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8341 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     8401 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8461 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8521 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8581 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8641 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8701 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8761 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     8821 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     8881 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     8941 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     9001 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9061 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9121 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9181 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9241 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9301 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9361 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9421 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9481 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9541 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9601 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9661 cagacccacc tcccaacccc gaggggaccc agagagggcc tattttaaaa gaaaaggggg
     9721 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     9781 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     9841 cagagatcca ctttggcgcc ggctcgag
//
