LOCUS       sequence-477852-        5427 bp    DNA     circular SYN 07-JUL-2025
DEFINITION  Gateway entry clone with CG6867 without stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5427)
  TITLE     Perrimon Lab Plasmids 2025
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTR_CG6867_nostop
FEATURES             Location/Qualifiers
     source          1..5427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3552..3651)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3668..3687)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3669..3687)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3692..3708)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3692..3708)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3810..3829)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3821..4630
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3888..3907)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4777..5365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5266..5285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccat gtttctggcg gttaaccaga gtctacagaa
      721 taccagcaag aagaagcgca agcggcggac ggccgaggat gatccgcagg aggattctgg
      781 agaaggaacg ccaccagttc gtcccctgga acccagtgcg tccagtgacc agttggtcgg
      841 agttggcaat ggagccactt gccgggtgtg taagtgcccg ggcaggcagt ccttgaccct
      901 ggtatacatc tacctaggcg ccctcaccct catcctggcc agcctctcaa tcgtcttcta
      961 cacgcacacc caggacgtcg agtccttgtc acaggtggaa gataactatt cgtcgaaatc
     1021 gttccgggta caattccagg cggagttaat gcgctcggaa gaggaattaa ggagactggt
     1081 caacaggata atcgaggagg agcaggaatt ggttggcagc accaccgcga gtagcaccac
     1141 ccaatcgagg gatcctctga agaatgaacg caagttcacg gataacctga ttagccacac
     1201 aacgcgtccc acagggaaac gaatgcgccg ggacatcgcc tcctccgctc cggcatccat
     1261 gcatgctgat ccgctgatcg agttcttcaa tccgaaccat cgcaaggttt tggaggagca
     1321 agacaccgag atccgtaagc gcacgggtca aaagggcgcc gctcccggcg gagatgagtg
     1381 gatctatctg aacacatact gccgtgttcc ggagaagata atcacgggct tctgcaaggg
     1441 tacccaggac tattgtccgc cggcaccgca aggaccaacc ggagaagtgg gaccaaaggg
     1501 accaccggga aacccgggtc tacctggcat ccccggtcca aagggaaatc gcggtgatgt
     1561 gggattaccg ggagcccccg gtgtggatgg cgtgggtcat ttgggtcccg ccggacctcg
     1621 cggtcctaag ggagatgccg gggccaccgg aagagccggc ttggatggac gggatggagt
     1681 gcccggggag ccgggactcg atggcgtacc gggtcgcgct ggtgctgatg gaaagaatgg
     1741 cctgcctgga cgcgatggca aggatgggct acacggcaag gatggcaaag atggtctgtc
     1801 catcacggga cccaagggtg cgcagggacc accaggcgaa cgaggtctca agggcattgc
     1861 gggtccacgt ggtcgacctg gcaagccggg aaccaatggc atacccggag tgcccggcat
     1921 caatgcctgg aagctgcaat atcccaatgg cagctcctcc aacgatctgc tcataccgcc
     1981 ttctataaca gacatccaag tgccggactt ccaacgcacg gtgattgtgg aggagggaag
     2041 atccctgaat ttgagctgca ccgccacggg aactccgaca ccgcaggtgg aatggcgacg
     2101 tgaggatggt cgcaccatta atgtcaatgg cgtagagatg gcctccatca gcggacagtt
     2161 cctgagattc accaatatca cccgacatca gatggcggcc tacacctgtt ttgccaacaa
     2221 tggcatcgct cccgtggcaa atgccaccta cctcgtggaa gtgcaatttg ctcccatgat
     2281 atcggtgtat cgccaaatga tctacgcgga ataccagagc agtgccacac tcgagtgttt
     2341 ggtggaggca ttccccgaag ccattaggta ttgggaacgt gcctacgacg gcaagatcct
     2401 cgatcccagc gacaagtacg gcattgaatc gtacccagag ggtttcaaga caaccatgcg
     2461 cttgaccatc agtaatctgc gtaaggatga ctttgggtac tatcattgtg tggcccgaaa
     2521 tgaactaaat gccacaatgg tcaactttga aatagcaccc caagatccga atagtgagac
     2581 cccgtatgtg ggaaataaca tgaaggtata cggtcaaaga ccgccggaga gcgagtgtcc
     2641 cgtttgcgat cagtgtccag atcccagctt gtaccagtgc aaggactcca tactgaataa
     2701 ctttgaaatc caggccacgg gcaatctcag ctatccggga ttacccaagc gaccaaagac
     2761 ttgctatctg tatgccgtgg gcaagcccgt tttccacaag gtggtcaatg aaaagttcgg
     2821 ctcctggctt agagatccat ctccggacag tgatcgtgag aagacgttcg tcactaacga
     2881 aaacgatccg tacaatctgt ttgagttcac cactcgcatt cagtaccgaa tgaatagcat
     2941 cccccggagg aaatacgaga ttcaagaagg ctttcatggc aatgcacatg tggtctttaa
     3001 cggttcgttt tactatcaac aaagaaactc ggatctggtg gtcaagctgg acttgaccag
     3061 cctgaaaaag ataaccacac aattgccata tgctggagtg gctgctgcga ataggcttta
     3121 taccacggac tacaactaca tggacttcaa tgtggatgaa gtgggcctgt gggtgatcta
     3181 tagcacctac aactccaaca atacgctggt ggccaagttg gatgcggaga cgttgaagat
     3241 gcagtacaac tttaatatca ccctggacca tcatcagttt ggtgagatgt tcatcgtgtg
     3301 tggcaatctg tatgccatcg attcgggaac ggacaagaac acccagattc gatatgtagt
     3361 ggacttgtac aagggcaagc tgctcaacac gaatctgccc ttctcgaatc cctttagcca
     3421 taccaccacc gtgggctaca atcccctgac tgtggaactc tactcctggg acaagggcaa
     3481 cgcactcaca tatcccatac gctacaatga gcagcgttta atctccgaca atagtaaggg
     3541 tgggcgcgcc gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     3601 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     3661 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     3721 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     3781 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     3841 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     3901 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     3961 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     4021 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     4081 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     4141 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     4201 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     4261 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     4321 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     4381 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     4441 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     4501 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     4561 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     4621 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     4681 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     4741 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4801 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4861 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4921 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4981 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5041 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5101 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5161 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5221 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5281 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5341 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5401 cttttgctgg ccttttgctc acatgtt
//
