LOCUS       sequence-477867-        8682 bp    DNA     circular SYN 08-JUL-2025
DEFINITION  Used for transfection of cells for expression of C-MYC PDZD8 (with 
            deletion of PDZ aa 367-449).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pcDNA 3.3 C-MYC PDZD8(Delta-PDZ 367-449aa)
FEATURES             Location/Qualifiers
     source          1..8682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3993..4022
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4068..4087)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4112..4160
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4362..4790
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4449..4468)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4659..4680
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4799..4819)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4804..5133
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4984..5119
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5046..5065
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5200..5994
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5254..5273)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5864..5883
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6170..6291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6207..6226)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6261..6280
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6340..6356)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6340..6356)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6353..6375)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6364..6380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6388..6394,6395..6412,6413..6418))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6433..6454
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6571..6588)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6742..7330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6822..6841)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7501..8292,8293..8361))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8124..8143
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8362..8466)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8541..8560)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actgagctcc ttaaggttaa ctctagagaa ttcgatccat ggggctgctg ctcatgatcc
      781 tggcgtcggc cgtgctgggt tccttcctca cgctcctcgc ccagttcttc ctgctgtacc
      841 gcagacagcc cgagccgccg gcggacgagg ccgcccgcgc gggcgagggc ttccgctaca
      901 tcaagccagt gccgggcctg ctcctaaggg agtaccttta tggcggcggc cgggatgagg
      961 agccctccgg agcggcccct gagggcggcg cgacccccac cgcggccccc gagacccccg
     1021 ccccgccgac gcgggagact tgctacttcc tcaacgccac catcctattc ctgttccggg
     1081 agttgcggga caccgcgctg acccgccgct gggtcaccaa gaagatcaag gtggagttcg
     1141 aggagctgct gcagaccaag acggccgggc gcctgctgga ggggctgagc ctgcgggacg
     1201 tgttcctggg cgagacggtg cccttcatca agaccatccg gctcgtgcgg ccagtcgtgc
     1261 cctcggccac cggggagccc gatggccctg aaggggaggc gctgcccgcc gcctgccccg
     1321 aggagctggc cttcgaggcg gaggtggagt acaacggggg cttccacctg gccatcgacg
     1381 tggacctggt cttcggcaag tccgcctact tgtttgtcaa gctgtcccgc gtggtgggaa
     1441 ggctgcgctt ggtctttacg cgcgtgccct tcacccactg gttcttctcc ttcgtggaag
     1501 acccgctgat cgacttcgag gtgcgctccc agtttgaagg gcggcccatg ccccagctca
     1561 cctccatcat cgtcaaccag ctcaagaaga tcatcaagcg caagcacacc ctaccgaatt
     1621 acaagatcag gtttaagccg ttttttccat accagacctt gcaaggattt gaagaagatg
     1681 aagagcatat ccatatacaa caatgggcac ttactgaagg ccgtcttaaa gttacgttgt
     1741 tagaatgtag caggttactc atttttggat cctatgacag agaggcaaat gttcattgta
     1801 cacttgagtt aagcagtagt gtttgggaag aaaaacagag gagttctatt aagacgggcc
     1861 agagtaatca aggtgcagtg ctgcaagata actttggcca gttggaagaa aactttttgt
     1921 caagctcatg ccaatcgggt tatgaagagg aagctgccgg gttgacagta gatactgaaa
     1981 gtagagagct ggattctgaa tttgaagact tggcaagtga tgtcagagca caaaatgagt
     2041 tcaaagatga ggcacaatca ttaagtcata gtcccaaacg tgttccaaca acactttcta
     2101 ttaaacccct tggagctata tcaccagttt taaaccgtaa attagctgta ggaagtcacc
     2161 cactaccacc gaaaattcag tccaaagatg gaaataaacc tccaccccta aaaacttctg
     2221 agataacaga cccagcacaa gtgtcaaaac caacccaagg atctgctttc aaaccacctg
     2281 tgccaccacg accacaagcg aaagttcctt tgccttccgc cgatgctcca aatcaggcag
     2341 aaccagatgt tctcgttgaa aagccagaga aggtggtgcc acctcctctt gtagataaat
     2401 ctgctgaaaa gcaagcaaaa aatgtggatg ccatagacga tgcagctgca cctaagcaat
     2461 ttttagcaaa gcaagaagtg gccaaagatg tcacttcaga aacttcctgc cctactaagg
     2521 acagttcgga cgaccgtcaa acatgggaat catcagaaat tctttatcgt aataagctag
     2581 gaaaatggac aagaaccaga gcatcctgtt tgtttgacat agaagcctgt cacaggtact
     2641 taaacattgc attgtggtgc agggatcctt tcaagttggg aggtctcatc tgtttggggc
     2701 atgttagttt aaaacttgaa gatgtggctt taggatgcct agctacatca aacacggaat
     2761 acctttccaa attgagactg gaagccccct cacctaaggc tatagtcact agaaccgcac
     2821 tacgcaatct gagtatgcaa aagggattca atgacaaatt ttgctatggt gacattacta
     2881 ttcacttcaa atatttgaaa gaaggagaat cagaccacca tgtagttact aacgtagaaa
     2941 aagaaaaaga accccatttg gttgaagaag tttctgttct ccctaaagag gagcaatttg
     3001 ttggacagat gggtttaaca gaaaacaaac acagttttca ggatactcag ttccagaacc
     3061 caacatggtg tgactactgt aagaaaaaag tttggactaa agcagcttcc cagtgtatgt
     3121 tttgtgctta tgtttgccat aaaaaatgtc aagaaaagtg tctagctgag acttctgttt
     3181 gtggagcaac tgataggcga atagacagga cactgaaaaa ccttaggctg gaaggacagg
     3241 aaaccctctt aggcctgcct cctcgtgttg atgctgaagc tagcaagtca gtcaataaaa
     3301 caacaggttt gacaaggcat attatcaata ctagttctcg tttattaaat ttgcgtcaag
     3361 tctctaaaac tcgcctttct gaaccaggaa ccgatctcgt agaaccttca ccaaaacaca
     3421 cacccaacac gtcagacaac gaaggcagtg acacggaggt ctgtggtcca aacagtcctt
     3481 ctaaacgggg aaacagcaca ggaataaagt tagtgagaaa agagggtggt ctggatgaca
     3541 gtgttttcat tgcagttaaa gaaattggtc gtgatctgta caggggcttg cctacagagg
     3601 aaaggatcca gaaactagag ttcatgttgg ataagctaca gaatgaaatt gatcaggagt
     3661 tggaacacaa taattccctt gttagagaag aaaaagagac aactgataca aggaaaaaat
     3721 cacttctttc tgctgcctta gctaaatcag gtgaaaggct acaagctcta acacttctta
     3781 tgattcacta cagagcaggc attgaagata tagaaacttt agaaagtctg tctttagacc
     3841 agcactccaa aaaaataagc aagtacacag atgatacaga agaagacctt gataatgaaa
     3901 taagccaact aatagactct cagccattca gcagcatatc agatgactta tttggcccat
     3961 ccgagtctgt gctcgagcca tggaagctta tggaacaaaa actcatctca gaagaggatc
     4021 tggatatcta actgactgaa ccggtggtcc aaacggggga ggctaactga aacacggaag
     4081 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     4141 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     4201 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     4261 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     4321 catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4381 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4441 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4501 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4561 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4621 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4681 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4741 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     4801 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4861 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4921 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4981 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5041 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5101 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5161 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     5221 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     5281 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5341 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     5401 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5461 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5521 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5581 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5641 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5701 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     5761 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5821 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5881 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5941 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     6001 ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     6061 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6121 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     6181 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6241 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6301 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6361 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6421 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6481 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6541 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7141 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8461 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     8521 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     8581 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     8641 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gg
//
