LOCUS       sequence-477869-        7762 bp    DNA     circular SYN 08-JUL-2025
DEFINITION  Used for transfection of cells for expression of C-MYC PDZD8 (with 
            deletion of aa 451-839).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pcDNA 3.3 C-MYC PDZD8(Delta-451-839aa)
FEATURES             Location/Qualifiers
     source          1..7762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3073..3102
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3148..3167)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3192..3240
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3442..3870
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3529..3548)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3739..3760
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3879..3899)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3884..4213
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4064..4199
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4126..4145
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4280..5074
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4334..4353)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4944..4963
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5250..5371
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5287..5306)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5341..5360
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5420..5436)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5420..5436)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5433..5455)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5444..5460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5468..5474,5475..5492,5493..5498))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5513..5534
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5651..5668)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5822..6410)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5902..5921)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6581..7372,7373..7441))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7204..7223
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7442..7546)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7621..7640)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actgagctcc ttaaggttaa ctctagagaa ttcggatcca tggggctgct gctcatgatc
      781 ctggcgtcgg ccgtgctggg ttccttcctc acgctcctcg cccagttctt cctgctgtac
      841 cgcagacagc ccgagccgcc ggcggacgag gccgcccgcg cgggcgaggg cttccgctac
      901 atcaagccag tgccgggcct gctcctaagg gagtaccttt atggcggcgg ccgggatgag
      961 gagccctccg gagcggcccc tgagggcggc gcgaccccca ccgcggcccc cgagaccccc
     1021 gccccgccga cgcgggagac ttgctacttc ctcaacgcca ccatcctatt cctgttccgg
     1081 gagttgcggg acaccgcgct gacccgccgc tgggtcacca agaagatcaa ggtggagttc
     1141 gaggagctgc tgcagaccaa gacggccggg cgcctgctgg aggggctgag cctgcgggac
     1201 gtgttcctgg gcgagacggt gcccttcatc aagaccatcc ggctcgtgcg gccagtcgtg
     1261 ccctcggcca ccggggagcc cgatggccct gaaggggagg cgctgcccgc cgcctgcccc
     1321 gaggagctgg ccttcgaggc ggaggtggag tacaacgggg gcttccacct ggccatcgac
     1381 gtggacctgg tcttcggcaa gtccgcctac ttgtttgtca agctgtcccg cgtggtggga
     1441 aggctgcgct tggtctttac gcgcgtgccc ttcacccact ggttcttctc cttcgtggaa
     1501 gacccgctga tcgacttcga ggtgcgctcc cagtttgaag ggcggcccat gccccagctc
     1561 acctccatca tcgtcaacca gctcaagaag atcatcaagc gcaagcacac cctaccgaat
     1621 tacaagatca ggtttaagcc gttttttcca taccagacct tgcaaggatt tgaagaagat
     1681 gaagagcata tccatataca acaatgggca cttactgaag gccgtcttaa agttacgttg
     1741 ttagaatgta gcaggttact catttttgga tcctatgaca gagaggcaaa tgttcattgt
     1801 acacttgagt taagcagtag tgtttgggaa gaaaaacaga ggagttctat taagacggtt
     1861 gaattaataa aaggaaattt acaaagtgtt ggacttacac ttcgtcttgt ccagtcaact
     1921 gatgggtatg ctgggcacgt catcattgaa actgtggctc caaactcgcc tgctgcaatt
     1981 gcagatcttc agcggggaga tcgacttatc gccattggag gtgtgaaaat cacatcaaca
     2041 ctgcaagtgt tgaagcttat caagcaggct ggtgaccgag tcctggtgta ctatgaaagg
     2101 cctgttaaac acagttttca ggatactcag ttccagaacc caacatggtg tgactactgt
     2161 aagaaaaaag tttggactaa agcagcttcc cagtgtatgt tttgtgctta tgtttgccat
     2221 aaaaaatgtc aagaaaagtg tctagctgag acttctgttt gtggagcaac tgataggcga
     2281 atagacagga cactgaaaaa ccttaggctg gaaggacagg aaaccctctt aggcctgcct
     2341 cctcgtgttg atgctgaagc tagcaagtca gtcaataaaa caacaggttt gacaaggcat
     2401 attatcaata ctagttctcg tttattaaat ttgcgtcaag tctctaaaac tcgcctttct
     2461 gaaccaggaa ccgatctcgt agaaccttca ccaaaacaca cacccaacac gtcagacaac
     2521 gaaggcagtg acacggaggt ctgtggtcca aacagtcctt ctaaacgggg aaacagcaca
     2581 ggaataaagt tagtgagaaa agagggtggt ctggatgaca gtgttttcat tgcagttaaa
     2641 gaaattggtc gtgatctgta caggggcttg cctacagagg aaaggatcca gaaactagag
     2701 ttcatgttgg ataagctaca gaatgaaatt gatcaggagt tggaacacaa taattccctt
     2761 gttagagaag aaaaagagac aactgataca aggaaaaaat cacttctttc tgctgcctta
     2821 gctaaatcag gtgaaaggct acaagctcta acacttctta tgattcacta cagagcaggc
     2881 attgaagata tagaaacttt agaaagtctg tctttagacc agcactccaa aaaaataagc
     2941 aagtacacag atgatacaga agaagacctt gataatgaaa taagccaact aatagactct
     3001 cagccattca gcagcatatc agatgactta tttggcccat ccgagtctgt gctcgagcca
     3061 tggaagctta tggaacaaaa actcatctca gaagaggatc tggatatcta actgactgaa
     3121 ccggtggtcc aaacggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     3181 ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
     3241 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     3301 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     3361 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcagat ctgcgcagct
     3421 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3481 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3541 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3601 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     3661 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3721 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3781 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3841 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     3901 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3961 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4021 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4081 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4141 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4201 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     4261 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4321 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4381 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     4441 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     4501 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     4561 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     4621 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     4681 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     4741 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     4801 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     4861 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     4921 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     4981 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5041 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga
     5101 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5161 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5221 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     5281 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5341 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     5401 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5461 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5521 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5581 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5641 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5701 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5761 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5821 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5881 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5941 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6001 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6061 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6121 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6181 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6241 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6301 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6361 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6421 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6481 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6541 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6601 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6661 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6721 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6781 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6841 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6901 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6961 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7021 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7081 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7141 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7201 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7261 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7321 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7381 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7441 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7501 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7561 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     7621 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     7681 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     7741 ttgcatgaag aatctgctta gg
//
