LOCUS       sequence-477876-       10664 bp    DNA     circular SYN 08-JUL-2025
DEFINITION  Used for transfection of cells for expression of C-MYC TB182.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pcDNA 3.3 N-MYC TB182
FEATURES             Location/Qualifiers
     source          1..10664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      742..751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             751..780
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(6050..6069)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6094..6142
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6344..6772
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6431..6450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6641..6662
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6781..6801)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6786..7115
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6966..7101
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7182..7976
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7236..7255)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7846..7865
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8152..8273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8189..8208)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8243..8262
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8322..8338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8322..8338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8335..8357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8346..8362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8370..8376,8377..8394,8395..8400))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8415..8436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8553..8570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8724..9312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8804..8823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9483..10274,10275..10343))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10106..10125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10344..10448)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10523..10542)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actgagctcc ttaaggttaa cgccaccatg gaacaaaaac tcatctcaga agaggatctg
      781 tctagagaat tcggatccat gaaagtgtct actctcaggg aaagctcagc catggcttcc
      841 ccactgcccc gggagatgga ggaggagctg gtgcctactg gctctgagcc aggtgacact
      901 cgggccaaac cccctgtcaa gcccaaaccc cgggccctgc ctgccaagcc agccctgcct
      961 gccaaaccca gcctgctggt gcctgttggg cctcggcctc cccggggtcc cctggctgag
     1021 ttgccttctg ccaggaagat gaacatgctg gcaggacccc agccctatgg tggcagcaag
     1081 cgcccccttc cctttgcacc aaggcctgcg gttgaggcct ccactggagg agaagccacc
     1141 caagagactg ggaaagagga ggctgggaaa gaggagccac cccctttgac acccccagct
     1201 cgatgtgcag ccccaggggg tgtacggaag gcccctgccc ctttccgccc agcctcagag
     1261 cgcttcgcgg ccaccacggt ggaagagatc ctggccaaga tggagcagcc tcggaaggag
     1321 gtccttgcca gccccgaccg cctgtggggt tcccgcctca cctttaacca cgatggcagc
     1381 tcgcgatatg gccccaggac ctatggcacg accactgctc ccagggatga ggatggcagc
     1441 accctcttca ggggatggtc ccaggagggg ccagtaaagt ctccagcaga gtgccgggaa
     1501 gagcacagca agacccctga ggagaggagc cttccttccg acctggcctt caacggggac
     1561 ctggctaagg cagccagctc ggagctacct gctgatattt ccaagccccg gattccctca
     1621 agtccagccc cctcctcaga gaatggaggc cctgccagcc caggcctccc cgcagaagcc
     1681 tcaggctcag gccctggctc tccccatctt cacccgcctg ataagagttc tccctgccac
     1741 tcacagcttc tggaagccca gactcctgaa gcttcccagg cttctccctg ccccgctgtg
     1801 actccatcag ctccaagtgc agccctgcct gacgagggct cccgccacac ccccagcccg
     1861 gggctccctg ccgagggggc tccagaggcc cccagaccca gcagcccacc ccctgaggtc
     1921 ttggagcccc atagcctgga tcagccccct gccacctcac cccggcccct gatcgaggtg
     1981 ggtgagttgc tggatctcac tcggacgttt ccatctggcg gggaggagga ggccaagggt
     2041 gacgcacacc tccgccccac cagcctggtt cagcgccgat tctctgaagg tgtgctccag
     2101 tcacccagtc aggaccagga gaagctaggg ggctcgctgg ctgccctgcc ccaaggccag
     2161 gggagccagt tggccctgga tcgtcccttt ggggcagagt ccaactggag cttatcacag
     2221 tccttcgaat ggaccttccc cacgaggccc tcgggtctgg gcgtgtggcg gctggactcc
     2281 ccgcctccct cccccatcac tgaagccagt gaggccgccg aggctgctga ggctggcaac
     2341 ttggccgttt ccagcaggga agaaggagtg tctcagcagg ggcaaggggc tgggtcagct
     2401 ccaagtgggt caggaagttc ctgggtgcag ggggatgatc caagcatgtc cctcacccag
     2461 aagggcgatg gggagagtca acctcaattc ccagctgttc cccttgagcc cctgcctaca
     2521 actgagggca cacctggatt acctttgcag caggcagagg agagatacga gtcgcaggag
     2581 cccttggctg gacaggagtc ccctctcccc ctggctacca gggaggcagc cttgcccatc
     2641 ctggagccag tcctggggca ggagcagcca gcagcccctg accagccctg tgttctcttt
     2701 gctgatgccc ctgagcctgg acaggcactg cctgttgagg aggaggccgt gaccctagcc
     2761 cgggctgaga ccacccaagc caggacagag gctcaagact tgtgtagggc atcccccgag
     2821 cctccaggcc ctgaaagcag ctcccgctgg ctggacgacc tcctggcttc accaccaccc
     2881 agtggtggcg gtgcaaggcg gggagctgga gctgagctga aggacacaca gtccccaagt
     2941 acctgctctg agggactcct tggctggtcc cagaaagatc tgcagagtga atttgggatc
     3001 acaggagacc cacagcccag cagtttcagt ccttccagct ggtgtcaagg tgcttctcag
     3061 gactatggcc ttgggggtgc aagccctaga ggagacccag gtctcggaga gagggactgg
     3121 accagcaagt atgggcaaga agcaggggaa gggagcacca gggagtgggc cagcaggtgt
     3181 ggcatcggcc aggaggagat ggaggccagc agcagccaag accagagtaa agtgtctgcc
     3241 ccaggggtgc tcacagccca ggaccgggta gttggaaagc cagcccagct tggcactcag
     3301 cggagccagg aggcagatgt tcaggactgg gagttcagaa agagggattc ccagggcact
     3361 tactccagcc gggatgcaga actccaggac caggaattcg gaaagagaga ttcactgggt
     3421 acctacagta gtcgagatgt aagccttggg gactgggaat ttgggaagag agattctctg
     3481 ggtgcttatg ccagccaaga tgccaacgag cagggccaag atttggggaa gagggaccac
     3541 catggtaggt acagcagcca ggatgccgat gagcaggact gggagtttca gaagagagat
     3601 gtgtcactcg gcacctatgg cagccgggct gcggagccac aggaacagga gtttgggaag
     3661 agcgcttgga taagggacta cagcagtggt gacagctcca ggacccttga cgcccaggac
     3721 agaagctttg gaacgagacc cctgagctct gggttcagcc ccgaggaagc ccagcaacag
     3781 gatgaggaat ttgagaagaa gattccaagt gtggaagaca gccttggaga gggcagcagg
     3841 gatgctggcc ggccaggaga gagaggatcc gggggcttgt tcagtcctag cactgcccac
     3901 gtgccggatg gggcactcgg gcagagagac cagagcagct ggcaaaacag tgatgctagc
     3961 caggaggtgg gagggcatca ggagagacag caggcagggg ctcagggccc tggcagtgct
     4021 gacctggaag atggggagat gggaaagcga ggctgggtcg gtgagtttag cctcagtgtt
     4081 ggcccccagc gagaggcagc atttagccca gggcagcagg actggagccg ggacttctgc
     4141 atcgaggcca gtgagaggag ctatcagttt ggcatcattg gcaacgacag agtgagtggt
     4201 gctggcttta gcccttctag caagatggaa ggtggtcact ttgtgcctcc tgggaagacc
     4261 acagctggct cggtggactg gactgaccag ctgggtctca ggaacttgga agtgtccagc
     4321 tgtgtgggtt ctgggggctc gagcgaggcc agggagagtg ccgtgggaca gatgggctgg
     4381 tcaggtggcc tgagcttgag agacatgaac ctgaccggct gtttggaaag tggagggtct
     4441 gaagagccgg ggggaatcgg agttggggag aaggactgga cttctgatgt taatgtgaag
     4501 agcaaagatt tggctgaggt cggggaggga ggaggccaca gccaggccag agagagtggc
     4561 gtggggcaga ctgactggtc aggtgtggag gccggagagt tccttaaatc aagggagcgt
     4621 ggagttggac aggcagactg gacacctgac cttgggctga gaaacatggc cccaggggca
     4681 gtctgcagtc ctggagagtc caaagagctt ggggtgggcc agatggactg gggtaacaat
     4741 ctgggcctga gggatttgga ggtgacctgt gacccagact ctggaggttc tcaggggcta
     4801 cggggatgtg gagtggggca gatggactgg acccaggact tggcgcccca gaatgtggag
     4861 ctctttgggg ctccaagtga agccagggag catggggtgg gcggggtgag ccagtgccca
     4921 gagcccggcc tgaggcacaa tggcagcttg tctcctggcc tggaggccag agaccccttg
     4981 gaggccaggg agctgggggt tggtgagaca agtgggccag agacccaggg tgaagattac
     5041 tcctcgtctt ccttggagcc acaccctgca gaccctggaa tggagacagg agaagccctc
     5101 agcttcggag caagccctgg caggtgcccg gcccgccccc caccctccgg ctcccagggc
     5161 ctgctggagg agatgctggc agccagcagc tccaaggcgg tggctcggag ggagtcagcg
     5221 gcctcgggcc ttgggggcct gttggaggag gaaggagccg gggcaggtgc tgcccaagag
     5281 gaggtgctgg agcctggcag ggactctcca ccctcctgga ggccgcagcc tgatggtgag
     5341 gccagccaga cagaagacgt ggatggcacc tggggctctt cagcagccag gtggagcgat
     5401 caggggccag cacagacttc tcggcgaccc tcccaaggcc ctcctgccag atcccccagt
     5461 caggacttct ccttcattga ggacaccgag atcctcgaca gtgccatgta tcggagccgt
     5521 gccaacttgg ggcgcaagcg tgggcaccgg gccccggtca ttcggcctgg gggtaccttg
     5581 ggcctgtcgg aggcagcaga ctcggatgca cacctgttcc aggactctac agagccacgg
     5641 gcatctcggg tgccatcttc agatgaagag gtagtggagg aacctcagag ccgccggaca
     5701 cggatgtcgt tgggcaccaa ggggctgaaa gtcaacctct ttcctggcct gagcccctca
     5761 gccctgaagg ccaagctgcg cccccggaat cgctcagctg aggagggaga gctggctgag
     5821 agcaagtcga gccagaagga gtccgcggtc cagcgttcga aatcctgcaa ggtcccagga
     5881 ctgggaaagc ccctcacgtt acctcccaag ccagagaaat cctcagggtc agaaggatcg
     5941 tcgcccaact ggcttcaagc cctgaaactg aagaagaaga aggtcctcga gccatggaag
     6001 cttgatatct aactgactga accggtggta ccaaacgggg gaggctaact gaaacacgga
     6061 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     6121 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     6181 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     6241 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     6301 gccatagcag atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     6361 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6421 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6481 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6541 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6601 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6661 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6721 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     6781 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6841 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     6901 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6961 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7021 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     7081 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     7141 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     7201 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     7261 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     7321 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     7381 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     7441 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     7501 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     7561 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     7621 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     7681 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     7741 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     7801 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     7861 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     7921 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     7981 gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     8041 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     8101 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     8161 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8221 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8281 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     8341 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8401 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8461 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8521 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8581 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8641 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8701 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8761 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8821 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8881 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8941 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9001 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9061 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9121 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     9181 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9241 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9301 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9361 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9421 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9481 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9541 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9601 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9661 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9721 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9781 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9841 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9901 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9961 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10021 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    10081 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    10141 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10201 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10261 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10321 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10381 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10441 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
    10501 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
    10561 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
    10621 caaggcaagg cttgaccgac aattgcatga agaatctgct tagg
//
