LOCUS       sequence-477879-        7264 bp    DNA     circular SYN 08-JUL-2025
DEFINITION  Used for transfection of cells for expression of N-MYC GAC-WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pcDNA 3.3 C-MYC GAC-WT
FEATURES             Location/Qualifiers
     source          1..7264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2575..2604
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2650..2669)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2694..2742
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2944..3372
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3031..3050)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3241..3262
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3381..3401)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3386..3715
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3566..3701
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3628..3647
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3782..4576
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3836..3855)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4446..4465
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4752..4873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4789..4808)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4843..4862
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4922..4938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4922..4938)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4935..4957)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4946..4962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4970..4976,4977..4994,4995..5000))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5015..5036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5153..5170)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5324..5912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5404..5423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6083..6874,6875..6943))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLIGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6706..6725
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6944..7048)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7123..7142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actgagctcc ttaaggttaa ctctagagaa ttcggatcca tgatgcggct gcgaggctcg
      781 gggatgctgc gggacctgct cctgcggtcg cccgcaggag tatcggcgac tctgcggcgg
      841 gcacagccct tggtcaccct gtgccggcgt ccccgaggcg ggggacggcc ggccgcgggc
      901 ccggctgccg ccgcgcgact ccacccgtgg tggggcgggg gcggctggcc ggcggagccc
      961 ctcgcgcggg gcctgtccag ctctccttcg gagatcttgc aggagctggg caaggggagc
     1021 acgcatccgc agcccggggt gtcgccaccc gctgccccgg cggcgcccgg ccccaaggac
     1081 ggccccgggg agacggacgc gtttggcaac agcgagggca aagagctggt ggcctcaggt
     1141 gaaaataaaa taaaacaggg tctgttacct agcttggaag atttgctgtt ctatacaatt
     1201 gctgaaggac aagagaaaat acctgttcat aaatttatta cagcactcaa atctacagga
     1261 ttgcgaacgt ctgatcccag gttgaaagag tgtatggata tgttaagatt aactcttcaa
     1321 acaacatcag atggtgtcat gctagacaaa gatcttttta aaaaatgtgt tcagagcaac
     1381 attgttttgt tgacacaagc atttagaaga aagtttgtga ttcctgactt tatgtctttt
     1441 acctcacaca ttgatgagtt atatgaaagt gctaaaaagc agtctggagg aaaggttgca
     1501 gattatattc ctcaactggc caaattcagt cccgatttgt ggggtgtgtc tgtttgtaca
     1561 gtagatggac agaggcattc tactggagat accaaagttc ccttctgtct tcagtcctgt
     1621 gtaaaacctt tgaaatatgc cattgctgtt aatgatcttg gaactgaata tgtgcatcga
     1681 tatgttggaa aagagccgag tggactaaga ttcaacaaac tatttttgaa tgaagatgat
     1741 aaaccacata atcctatggt aaatgctgga gcaattgttg tgacttcact aataaagcaa
     1801 ggagtaaata atgctgaaaa atttgactat gtcatgcagt ttttgaataa gatggctggt
     1861 aatgaatatg ttggattcag taatgcaacg tttcagtctg aaagagaaag tggagatcga
     1921 aattttgcaa taggatatta cttaaaagaa aagaagtgtt ttccagaagg cacagacatg
     1981 gttggtatat tagacttcta cttccagctg tgctccattg aagtgacttg tgaatcagcc
     2041 agtgtgatgg ctgcgacact ggctaatggt ggtttctgcc caattactgg tgaaagagta
     2101 ctgagccctg aagcagttcg aaatacattg agtttgatgc attcctgtgg catgtatgac
     2161 ttctcagggc agtttgcttt ccatgttggt cttcctgcaa aatctggagt tgctgggggc
     2221 attcttttag ttgtccccaa tgttatgggt atgatgtgct ggtctcctcc tctggataag
     2281 atgggcaaca gtgttaaggg aattcacttt tgtcacgatc ttgtttctct gtgtaatttc
     2341 cataactatg ataatttgag acactttgca aaaaaacttg atcctcgaag agaaggtggt
     2401 gatcaaaggc attcctttgg accattggac tatgaaagtc tccaacaaga acttgcttta
     2461 aaagagacag tatggaaaaa agtgtcacct gagtcaaatg aggacatctc tacaactgta
     2521 gtatatagaa tggaaagtct gggagagaaa agcctcgagc catggaagct tatggaacaa
     2581 aaactcatct cagaagagga tctggatatc taactgactg aaccggtggt ccaaacgggg
     2641 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     2701 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     2761 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     2821 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     2881 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc
     2941 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3001 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3061 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3121 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3181 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3241 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3301 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3361 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     3421 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     3481 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3541 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3601 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     3661 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     3721 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     3781 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3841 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3901 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     3961 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4021 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4081 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4141 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4201 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4261 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     4321 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4381 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4441 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4501 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4561 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca
     4621 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     4681 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     4741 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4801 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4861 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     4921 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4981 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5041 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5101 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5161 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5221 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5281 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5341 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5401 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5461 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5521 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5581 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5641 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5701 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5761 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5821 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5881 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5941 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6001 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6061 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6121 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6181 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6241 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6301 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6361 cgccaattaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6421 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6481 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6541 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6601 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6661 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6721 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6781 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6841 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6901 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6961 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7021 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     7081 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     7141 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     7201 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     7261 tagg
//
