LOCUS       sequence-477893-       13720 bp    DNA     circular SYN 08-JUL-2025
DEFINITION  To amplify the virus that expresses nt-rfp-PDZD8 T527A ct-gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 13720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pBOBI nt-rfp-PDZD8 T527A ct-gfp
FEATURES             Location/Qualifiers
     source          1..13720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3226..3936
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             join(7318..7320,7321..7323,7324..8034)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7318..7320,7321..7323,7324..8034)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7363..7384)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7624..7643)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7971..7992
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          8121..8350
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     8333..8352
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    8527..9115
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8580..8600)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8998..9009)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9522..9702
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9728..9745)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9734..9958
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10004..10432
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10091..10110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10301..10322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10441..10461)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10446..10775
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10626..10761
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10688..10707
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10823..10870
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10889..11263
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11393..11514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11430..11449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11484..11503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11563..11579)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11563..11579)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11576..11598)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11587..11603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11611..11617,11618..11635,11636..11641))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11656..11677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11794..11811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11965..12553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12045..12064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12724..13515,13516..13584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13347..13366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13585..13689)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccatgggg ctgctgctca tgatcctggc gtcggccgtg
     3181 ctgggttcct tcctcacgct cctcgcccag ttcttcctgc tgtacatggt gtctaagggc
     3241 gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac
     3301 caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
     3361 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc
     3421 ttcatgtacg gcagcagaac cttcatcaac cacacccagg gcatccccga cttctttaag
     3481 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
     3541 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
     3601 agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag
     3661 gccaacaccg agatgctgta ccccgctgac ggcggcctgg aaggcagaag cgacatggcc
     3721 ctgaagctcg tgggcggggg ccacctgatc tgcaacttca agaccacata cagatccaag
     3781 aaacccgcta agaacctcaa gatgcccggc gtctactatg tggaccacag actggaaaga
     3841 atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac
     3901 tgcgacctcc ctagcaaact ggggcacaaa cttaatcgca gacagcccga gccgccggcg
     3961 gacgaggccg cccgcgcggg cgagggcttc cgctacatca agccagtgcc gggcctgctc
     4021 ctaagggagt acctttatgg cggcggccgg gatgaggagc cctccggagc ggcccctgag
     4081 ggcggcgcga cccccaccgc ggcccccgag acccccgccc cgccgacgcg ggagacttgc
     4141 tacttcctca acgccaccat cctattcctg ttccgggagt tgcgggacac cgcgctgacc
     4201 cgccgctggg tcaccaagaa gatcaaggtg gagttcgagg agctgctgca gaccaagacg
     4261 gccgggcgcc tgctggaggg gctgagcctg cgggacgtgt tcctgggcga gacggtgccc
     4321 ttcatcaaga ccatccggct cgtgcggcca gtcgtgccct cggccaccgg ggagcccgat
     4381 ggccctgaag gggaggcgct gcccgccgcc tgccccgagg agctggcctt cgaggcggag
     4441 gtggagtaca acgggggctt ccacctggcc atcgacgtgg acctggtctt cggcaagtcc
     4501 gcctacttgt ttgtcaagct gtcccgcgtg gtgggaaggc tgcgcttggt ctttacgcgc
     4561 gtgcccttca cccactggtt cttctccttc gtggaagacc cgctgatcga cttcgaggtg
     4621 cgctcccagt ttgaagggcg gcccatgccc cagctcacct ccatcatcgt caaccagctc
     4681 aagaagatca tcaagcgcaa gcacacccta ccgaattaca agatcaggtt taagccgttt
     4741 tttccatacc agaccttgca aggatttgaa gaagatgaag agcatatcca tatacaacaa
     4801 tgggcactta ctgaaggccg tcttaaagtt acgttgttag aatgtagcag gttactcatt
     4861 tttggatcct atgacagaga ggcaaatgtt cattgtacac ttgagttaag cagtagtgtt
     4921 tgggaagaaa aacagaggag ttctattaag acggttgaat taataaaagg aaatttacaa
     4981 agtgttggac ttacacttcg tcttgtccag tcaactgatg ggtatgctgg gcacgtcatc
     5041 attgaaactg tggctccaaa ctcgcctgct gcaattgcag atcttcagcg gggagatcga
     5101 cttatcgcca ttggaggtgt gaaaatcaca tcaacactgc aagtgttgaa gcttatcaag
     5161 caggctggtg accgagtcct ggtgtactat gaaaggcctg ttggccagag taatcaaggt
     5221 gcagtgctgc aagataactt tggccagttg gaagaaaact ttttgtcaag ctcatgccaa
     5281 tcgggttatg aagaggaagc tgccgggttg acagtagata ctgaaagtag agagctggat
     5341 tctgaatttg aagacttggc aagtgatgtc agagcacaaa atgagttcaa agatgaggca
     5401 caatcattaa gtcatagtcc caaacgtgtt ccagcaacac tttctattaa accccttgga
     5461 gctatatcac cagttttaaa ccgtaaatta gctgtaggaa gtcacccact accaccgaaa
     5521 attcagtcca aagatggaaa taaacctcca cccctaaaaa cttctgagat aacagaccca
     5581 gcacaagtgt caaaaccaac ccaaggatct gctttcaaac cacctgtgcc accacgacca
     5641 caagcgaaag ttcctttgcc ttccgccgat gctccaaatc aggcagaacc agatgttctc
     5701 gttgaaaagc cagagaaggt ggtgccacct cctcttgtag ataaatctgc tgaaaagcaa
     5761 gcaaaaaatg tggatgccat agacgatgca gctgcaccta agcaattttt agcaaagcaa
     5821 gaagtggcca aagatgtcac ttcagaaact tcctgcccta ctaaggacag ttcggacgac
     5881 cgtcaaacat gggaatcatc agaaattctt tatcgtaata agctaggaaa atggacaaga
     5941 accagagcat cctgtttgtt tgacatagaa gcctgtcaca ggtacttaaa cattgcattg
     6001 tggtgcaggg atcctttcaa gttgggaggt ctcatctgtt tggggcatgt tagtttaaaa
     6061 cttgaagatg tggctttagg atgcctagct acatcaaaca cggaatacct ttccaaattg
     6121 agactggaag ccccctcacc taaggctata gtcactagaa ccgcactacg caatctgagt
     6181 atgcaaaagg gattcaatga caaattttgc tatggtgaca ttactattca cttcaaatat
     6241 ttgaaagaag gagaatcaga ccaccatgta gttactaacg tagaaaaaga aaaagaaccc
     6301 catttggttg aagaagtttc tgttctccct aaagaggagc aatttgttgg acagatgggt
     6361 ttaacagaaa acaaacacag ttttcaggat actcagttcc agaacccaac atggtgtgac
     6421 tactgtaaga aaaaagtttg gactaaagca gcttcccagt gtatgttttg tgcttatgtt
     6481 tgccataaaa aatgtcaaga aaagtgtcta gctgagactt ctgtttgtgg agcaactgat
     6541 aggcgaatag acaggacact gaaaaacctt aggctggaag gacaggaaac cctcttaggc
     6601 ctgcctcctc gtgttgatgc tgaagctagc aagtcagtca ataaaacaac aggtttgaca
     6661 aggcatatta tcaatactag ttctcgttta ttaaatttgc gtcaagtctc taaaactcgc
     6721 ctttctgaac caggaaccga tctcgtagaa ccttcaccaa aacacacacc caacacgtca
     6781 gacaacgaag gcagtgacac ggaggtctgt ggtccaaaca gtccttctaa acggggaaac
     6841 agcacaggaa taaagttagt gagaaaagag ggtggtctgg atgacagtgt tttcattgca
     6901 gttaaagaaa ttggtcgtga tctgtacagg ggcttgccta cagaggaaag gatccagaaa
     6961 ctagagttca tgttggataa gctacagaat gaaattgatc aggagttgga acacaataat
     7021 tcccttgtta gagaagaaaa agagacaact gatacaagga aaaaatcact tctttctgct
     7081 gccttagcta aatcaggtga aaggctacaa gctctaacac ttcttatgat tcactacaga
     7141 gcaggcattg aagatataga aactttagaa agtctgtctt tagaccagca ctccaaaaaa
     7201 ataagcaagt acacagatga tacagaagaa gaccttgata atgaaataag ccaactaata
     7261 gactctcagc cattcagcag catatcagat gacttatttg gcccatccga gtctgtgatg
     7321 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     7381 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     7441 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     7501 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     7561 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     7621 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     7681 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     7741 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     7801 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     7861 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     7921 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     7981 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagctcgag
     8041 ccatggaagc ttgatatcta actgactgaa ccggtggtac cgaggaatta attcgctgtc
     8101 tgcgagggcc agctgttggg gtgagtactc cctctcaaaa gcgggcatga cttctgcgct
     8161 aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg cggtgatgcc
     8221 tttgagggtg gccgcgtcca tctggtcaga aaagacaatc tttttgttgt caagcttgag
     8281 gtgtggcagg cttgagatct ggccatacac ttgagtgaca atgacatcca ctttgccttt
     8341 ctctccacag gtgtccactc ccaggtccaa ctgcaggtcg agcatgcatc tagggcggcc
     8401 aattccgccg atcctggaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     8461 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     8521 tcagacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     8581 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     8641 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     8701 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     8761 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     8821 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     8881 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     8941 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     9001 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     9061 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     9121 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     9181 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     9241 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     9301 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     9361 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     9421 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     9481 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     9541 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9601 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9661 gatccctcag acccttttag tcagtgtgga aaatctctag cagggccgct ttaaacccgc
     9721 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     9781 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     9841 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     9901 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     9961 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
    10021 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    10081 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    10141 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    10201 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    10261 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    10321 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    10381 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
    10441 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    10501 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
    10561 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
    10621 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    10681 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
    10741 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
    10801 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
    10861 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
    10921 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
    10981 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
    11041 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
    11101 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
    11161 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
    11221 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
    11281 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    11341 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
    11401 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    11461 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    11521 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
    11581 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    11641 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    11701 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    11761 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    11821 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    11881 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    11941 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    12001 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    12061 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    12121 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    12181 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    12241 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    12301 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    12361 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    12421 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    12481 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    12541 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    12601 aaactcacgt taagggattt tggtcatgag attatcaaaa gggatcttca cctagatcct
    12661 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    12721 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    12781 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    12841 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    12901 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    12961 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    13021 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    13081 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    13141 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    13201 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    13261 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    13321 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    13381 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    13441 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    13501 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    13561 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    13621 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    13681 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
