LOCUS       sequence-477894-       10527 bp    DNA     circular SYN 08-JUL-2025
DEFINITION  Express 3xFlag N-terminal tagged human GPR75 p.Ala110fs mutant 
            transiently via transfection or stably via lentivirus-mediated 
            infection in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Jiang Y, Xun Y, Zhang Z
  TITLE     Central regulation of feeding and body weight by ciliary GPR75.
  JOURNAL   J Clin Invest. 2024 Aug 13;134(19):e182121. doi: 10.1172/JCI182121.
  PUBMED    39137039
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2024-08-13"; volume: "134(19):e182121. doi"; pages:
            " 10.1172/JCI182121"
COMMENT     Sequence Label: pBOB-3xFlag-hGpr75-p.Ala110fs
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3133..3142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3142..3207
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          4928..5157
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5140..5159
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5334..5922
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5387..5407)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5805..5816)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6329..6509
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6535..6552)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6541..6765
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6811..7239
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6898..6917)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7108..7129
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7248..7268)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7253..7582
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7433..7568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7495..7514
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7630..7677
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7696..8070
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8200..8321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8237..8256)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8291..8310
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8370..8386)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8370..8386)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8383..8405)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8394..8410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8418..8424,8425..8442,8443..8448))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8463..8484
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8601..8618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8772..9360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8852..8871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9531..10322,10323..10391))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10154..10173
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10392..10496)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgat
     3181 atcgattaca aggatgacga tgacaagtct agagaattcg gatccatgaa ctcaacaggc
     3241 caccttcagg atgcccccaa tgccacctcg ctccatgtgc ctcactcaca ggaaggaaac
     3301 agcacctctc tccaggaggg tcttcaggat ctcatccaca cagccacctt ggtgacctgt
     3361 acttttctac tggcggtcat cttctgcctg ggttcctatg gcaacttcat tgtcttcttg
     3421 tccttcttcg atccagcctt caggaaattc agaaccaact ttgatttcat gatcctgaac
     3481 ctgtccttct gtgacctctt catttgtgga gtgacagccc ccatgttcac ctttgtgtta
     3541 ttcttcagct cagtatcccg gatgctttct gcttcacttt ccatctcacc agttcaggct
     3601 tcatcatcat gtctctgaag acagtggcag tgatcgccct gcaccggctc cggatggtgt
     3661 tggggaaaca gcctaatcgc acggcctcct ttccctgcac cgtactcctc accctgcttc
     3721 tctgggccac cagtttcacc cttgccacct tggctacctt gaaaaccagc aagtcccacc
     3781 tctgtcttcc catgtccagt ctgattgctg gaaaagggaa agccattttg tctctctatg
     3841 tggtcgactt caccttctgt gttgctgtgg tctctgtctc ttacatcatg attgctcaga
     3901 ccctgcggaa gaacgctcaa gtcagaaagt gcccccctgt aatcacagtc gatgcttcca
     3961 gaccacagcc tttcatgggg gtccctgtgc agggaggtgg agatcccatc cagtgtgcca
     4021 tgccggctct gtataggaac cagaattaca acaaactgca gcacgttcag acccgtggat
     4081 ataccaagag tcccaaccaa ctggtcaccc ctgcagcaag ccgactccag ctcgtatcag
     4141 ccatcaacct ctccactgcc aaggattcca aagccgtggt cacctgtgtg atcattgtgc
     4201 tgtcagtcct ggtgtgctgt cttccactgg ggatttcctt ggtacaggtg gttctctcca
     4261 gcaatgggag cttcattctt taccagtttg aattgtttgg atttactctt atatttttca
     4321 agtcaggatt aaaccctttt atatattctc ggaacagtgc agggctgaga aggaaagtgc
     4381 tctggtgcct ccaatacata ggcctgggtt ttttctgctg caaacaaaag actcgacttc
     4441 gagccatggg aaaagggaac ctcgaagtca acagaaacaa atcctcccat catgaaacaa
     4501 actctgccta catgttatct ccaaagccac agaagaaatt tgtggaccag gcttgtggcc
     4561 caagtcattc aaaagaaagt atggtgagtc ccaagatctc tgctggacat caacactgtg
     4621 gtcagagcag ctcgaccccc atcaacactc ggattgaacc ttactacagc atctataaca
     4681 gcagcccttc ccaggaggag agcagcccat gtaacttaca gccagtaaac tcttttggat
     4741 ttgccaattc atatattgcc atgcattatc acaccactaa tgacttagtg caggaatatg
     4801 acagcacttc agccaagcag attccagtcc cctccgttta actcgagcca tggaagcttg
     4861 atatctaact gactgaaccg gtggtaccga ggaattaatt cgctgtctgc gagggccagc
     4921 tgttggggtg agtactccct ctcaaaagcg ggcatgactt ctgcgctaag attgtcagtt
     4981 tccaaaaacg aggaggattt gatattcacc tggcccgcgg tgatgccttt gagggtggcc
     5041 gcgtccatct ggtcagaaaa gacaatcttt ttgttgtcaa gcttgaggtg tggcaggctt
     5101 gagatctggc catacacttg agtgacaatg acatccactt tgcctttctc tccacaggtg
     5161 tccactccca ggtccaactg caggtcgagc atgcatctag ggcggccaat tccgccgatc
     5221 ctggaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     5281 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca gacaatcaac
     5341 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5401 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5461 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     5521 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     5581 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     5641 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     5701 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     5761 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     5821 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     5881 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt
     5941 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6001 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     6061 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     6121 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     6181 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     6241 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     6301 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     6361 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6421 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6481 cttttagtca gtgtggaaaa tctctagcag ggccgcttta aacccgctga tcagcctcga
     6541 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     6601 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     6661 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     6721 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     6781 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     6841 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6901 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6961 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7021 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7081 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7141 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7201 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     7261 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7321 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7381 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7441 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7501 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     7561 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     7621 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     7681 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     7741 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     7801 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     7861 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     7921 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     7981 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     8041 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     8101 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     8161 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     8221 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8281 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     8341 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8401 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8461 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8521 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8581 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8641 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8701 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8761 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8821 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8881 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8941 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9001 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9061 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9121 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9181 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9241 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9301 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9361 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9421 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9481 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9541 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9601 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9661 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9721 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9781 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9841 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9901 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9961 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10021 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10081 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10141 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10201 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10261 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10321 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10381 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10441 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10501 tttccccgaa aagtgccacc tgacgtc
//
