LOCUS       sequence-477895-        7987 bp    DNA     circular SYN 08-JUL-2025
DEFINITION  Used for transfection of cells for expression of C-MYC PDZD8 
            (1-839aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pcDNA 3.3 C-MYC PDZD8(1-839aa)
FEATURES             Location/Qualifiers
     source          1..7987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3298..3327
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3373..3392)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3417..3465
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3667..4095
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3754..3773)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3964..3985
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4104..4124)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4109..4438
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4289..4424
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4351..4370
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4505..5299
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4559..4578)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5169..5188
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5475..5596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5512..5531)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5566..5585
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5645..5661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5645..5661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5658..5680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5669..5685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5693..5699,5700..5717,5718..5723))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5738..5759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5876..5893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6047..6635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6127..6146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6806..7597,7598..7666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7429..7448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7667..7771)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7846..7865)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actgagctcc ttaaggttaa ctctagagaa ttcggatcca tggggctgct gctcatgatc
      781 ctggcgtcgg ccgtgctggg ttccttcctc acgctcctcg cccagttctt cctgctgtac
      841 cgcagacagc ccgagccgcc ggcggacgag gccgcccgcg cgggcgaggg cttccgctac
      901 atcaagccag tgccgggcct gctcctaagg gagtaccttt atggcggcgg ccgggatgag
      961 gagccctccg gagcggcccc tgagggcggc gcgaccccca ccgcggcccc cgagaccccc
     1021 gccccgccga cgcgggagac ttgctacttc ctcaacgcca ccatcctatt cctgttccgg
     1081 gagttgcggg acaccgcgct gacccgccgc tgggtcacca agaagatcaa ggtggagttc
     1141 gaggagctgc tgcagaccaa gacggccggg cgcctgctgg aggggctgag cctgcgggac
     1201 gtgttcctgg gcgagacggt gcccttcatc aagaccatcc ggctcgtgcg gccagtcgtg
     1261 ccctcggcca ccggggagcc cgatggccct gaaggggagg cgctgcccgc cgcctgcccc
     1321 gaggagctgg ccttcgaggc ggaggtggag tacaacgggg gcttccacct ggccatcgac
     1381 gtggacctgg tcttcggcaa gtccgcctac ttgtttgtca agctgtcccg cgtggtggga
     1441 aggctgcgct tggtctttac gcgcgtgccc ttcacccact ggttcttctc cttcgtggaa
     1501 gacccgctga tcgacttcga ggtgcgctcc cagtttgaag ggcggcccat gccccagctc
     1561 acctccatca tcgtcaacca gctcaagaag atcatcaagc gcaagcacac cctaccgaat
     1621 tacaagatca ggtttaagcc gttttttcca taccagacct tgcaaggatt tgaagaagat
     1681 gaagagcata tccatataca acaatgggca cttactgaag gccgtcttaa agttacgttg
     1741 ttagaatgta gcaggttact catttttgga tcctatgaca gagaggcaaa tgttcattgt
     1801 acacttgagt taagcagtag tgtttgggaa gaaaaacaga ggagttctat taagacggtt
     1861 gaattaataa aaggaaattt acaaagtgtt ggacttacac ttcgtcttgt ccagtcaact
     1921 gatgggtatg ctgggcacgt catcattgaa actgtggctc caaactcgcc tgctgcaatt
     1981 gcagatcttc agcggggaga tcgacttatc gccattggag gtgtgaaaat cacatcaaca
     2041 ctgcaagtgt tgaagcttat caagcaggct ggtgaccgag tcctggtgta ctatgaaagg
     2101 cctgttggcc agagtaatca aggtgcagtg ctgcaagata actttggcca gttggaagaa
     2161 aactttttgt caagctcatg ccaatcgggt tatgaagagg aagctgccgg gttgacagta
     2221 gatactgaaa gtagagagct ggattctgaa tttgaagact tggcaagtga tgtcagagca
     2281 caaaatgagt tcaaagatga ggcacaatca ttaagtcata gtcccaaacg tgttccaaca
     2341 acactttcta ttaaacccct tggagctata tcaccagttt taaaccgtaa attagctgta
     2401 ggaagtcacc cactaccacc gaaaattcag tccaaagatg gaaataaacc tccaccccta
     2461 aaaacttctg agataacaga cccagcacaa gtgtcaaaac caacccaagg atctgctttc
     2521 aaaccacctg tgccaccacg accacaagcg aaagttcctt tgccttccgc cgatgctcca
     2581 aatcaggcag aaccagatgt tctcgttgaa aagccagaga aggtggtgcc acctcctctt
     2641 gtagataaat ctgctgaaaa gcaagcaaaa aatgtggatg ccatagacga tgcagctgca
     2701 cctaagcaat ttttagcaaa gcaagaagtg gccaaagatg tcacttcaga aacttcctgc
     2761 cctactaagg acagttcgga cgaccgtcaa acatgggaat catcagaaat tctttatcgt
     2821 aataagctag gaaaatggac aagaaccaga gcatcctgtt tgtttgacat agaagcctgt
     2881 cacaggtact taaacattgc attgtggtgc agggatcctt tcaagttggg aggtctcatc
     2941 tgtttggggc atgttagttt aaaacttgaa gatgtggctt taggatgcct agctacatca
     3001 aacacggaat acctttccaa attgagactg gaagccccct cacctaaggc tatagtcact
     3061 agaaccgcac tacgcaatct gagtatgcaa aagggattca atgacaaatt ttgctatggt
     3121 gacattacta ttcacttcaa atatttgaaa gaaggagaat cagaccacca tgtagttact
     3181 aacgtagaaa aagaaaaaga accccatttg gttgaagaag tttctgttct ccctaaagag
     3241 gagcaatttg ttggacagat gggtttaaca gaaaacctcg agccatggaa gcttatggaa
     3301 caaaaactca tctcagaaga ggatctggat atctaactga ctgaaccggt ggtccaaacg
     3361 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     3421 aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc
     3481 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     3541 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     3601 ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg cagctggggc tctagggggt
     3661 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3721 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3781 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3841 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3901 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3961 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4021 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4081 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4141 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4201 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4261 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4321 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     4381 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     4441 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     4501 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4561 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4621 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4681 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4741 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4801 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4861 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4921 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4981 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     5041 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5101 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5161 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5221 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5281 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc
     5341 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     5401 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     5461 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5521 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5581 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     5641 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5701 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5761 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5821 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5881 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5941 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6001 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6061 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6121 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6181 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6241 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6301 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6361 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6421 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6481 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6541 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6601 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6661 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6721 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6781 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6841 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6901 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6961 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7021 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7081 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7141 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7201 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7261 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7321 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7381 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7441 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7501 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7561 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7621 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7681 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7741 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7801 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     7861 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     7921 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     7981 gcttagg
//
