LOCUS       sequence-477897-       10534 bp    DNA     circular SYN 08-JUL-2025
DEFINITION  Express 3xFlag N-terminal tagged wild-type human GPR75 transiently 
            via transfection or stably via lentivirus-mediated infection in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Jiang Y, Xun Y, Zhang Z
  TITLE     Central regulation of feeding and body weight by ciliary GPR75.
  JOURNAL   J Clin Invest. 2024 Aug 13;134(19):e182121. doi: 10.1172/JCI182121.
  PUBMED    39137039
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2024-08-13"; volume: "134(19):e182121. doi"; pages:
            " 10.1172/JCI182121"
COMMENT     Sequence Label: pBOB-3xFlag-hGpr75
FEATURES             Location/Qualifiers
     source          1..10534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..813
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             831..1011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1058..1183
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2390..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3057
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3132..3141
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3141..3206
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          4934..5163
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5146..5165
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5340..5928
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5393..5413)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5811..5822)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6335..6515
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6541..6558)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6547..6771
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6817..7245
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6904..6923)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7114..7135
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7254..7274)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7259..7588
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7439..7574
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7501..7520
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7636..7683
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7702..8076
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8206..8327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8243..8262)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8297..8316
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8376..8392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8376..8392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8389..8411)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8400..8416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8424..8430,8431..8448,8449..8454))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8469..8490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8607..8624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8778..9366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8858..8877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9537..10328,10329..10397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10160..10179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10398..10502)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
      841 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      901 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      961 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1021 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1081 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1141 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1201 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1261 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1321 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1381 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1441 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1501 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatcaact tttaaaagaa
     2401 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2461 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttaactctag agccaccatg gactacaaag accatgacgg tgattataaa gatcatgata
     3181 tcgattacaa ggatgacgat gacaagtcta gagaattcgg atccatgaac tcaacaggcc
     3241 accttcagga tgcccccaat gccacctcgc tccatgtgcc tcactcacag gaaggaaaca
     3301 gcacctctct ccaggagggt cttcaggatc tcatccacac agccaccttg gtgacctgta
     3361 cttttctact ggcggtcatc ttctgcctgg gttcctatgg caacttcatt gtcttcttgt
     3421 ccttcttcga tccagccttc aggaaattca gaaccaactt tgatttcatg atcctgaacc
     3481 tgtccttctg tgacctcttc atttgtggag tgacagcccc catgttcacc tttgtgttat
     3541 tcttcagctc agccagtagt atcccggatg ctttctgctt cactttccat ctcaccagtt
     3601 caggcttcat catcatgtct ctgaagacag tggcagtgat cgccctgcac cggctccgga
     3661 tggtgttggg gaaacagcct aatcgcacgg cctcctttcc ctgcaccgta ctcctcaccc
     3721 tgcttctctg ggccaccagt ttcacccttg ccaccttggc taccttgaaa accagcaagt
     3781 cccacctctg tcttcccatg tccagtctga ttgctggaaa agggaaagcc attttgtctc
     3841 tctatgtggt cgacttcacc ttctgtgttg ctgtggtctc tgtctcttac atcatgattg
     3901 ctcagaccct gcggaagaac gctcaagtca gaaagtgccc ccctgtaatc acagtcgatg
     3961 cttccagacc acagcctttc atgggggtcc ctgtgcaggg aggtggagat cccatccagt
     4021 gtgccatgcc ggctctgtat aggaaccaga attacaacaa actgcagcac gttcagaccc
     4081 gtggatatac caagagtccc aaccaactgg tcacccctgc agcaagccga ctccagctcg
     4141 tatcagccat caacctctcc actgccaagg attccaaagc cgtggtcacc tgtgtgatca
     4201 ttgtgctgtc agtcctggtg tgctgtcttc cactggggat ttccttggta caggtggttc
     4261 tctccagcaa tgggagcttc attctttacc agtttgaatt gtttggattt actcttatat
     4321 ttttcaagtc aggattaaac ccttttatat attctcggaa cagtgcaggg ctgagaagga
     4381 aagtgctctg gtgcctccaa tacataggcc tgggtttttt ctgctgcaaa caaaagactc
     4441 gacttcgagc catgggaaaa gggaacctcg aagtcaacag aaacaaatcc tcccatcatg
     4501 aaacaaactc tgcctacatg ttatctccaa agccacagaa gaaatttgtg gaccaggctt
     4561 gtggcccaag tcattcaaaa gaaagtatgg tgagtcccaa gatctctgct ggacatcaac
     4621 actgtggtca gagcagctcg acccccatca acactcggat tgaaccttac tacagcatct
     4681 ataacagcag cccttcccag gaggagagca gcccatgtaa cttacagcca gtaaactctt
     4741 ttggatttgc caattcatat attgccatgc attatcacac cactaatgac ttagtgcagg
     4801 aatatgacag cacttcagcc aagcagattc cagtcccctc cgtttaactc gagccatgga
     4861 agcttgatat ctaactgact gaaccggtgg taccgaggaa ttaattcgct gtctgcgagg
     4921 gccagctgtt ggggtgagta ctccctctca aaagcgggca tgacttctgc gctaagattg
     4981 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgcggtgat gcctttgagg
     5041 gtggccgcgt ccatctggtc agaaaagaca atctttttgt tgtcaagctt gaggtgtggc
     5101 aggcttgaga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
     5161 caggtgtcca ctcccaggtc caactgcagg tcgagcatgc atctagggcg gccaattccg
     5221 ccgatcctgg aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     5281 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcagaca
     5341 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5401 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5461 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5521 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5581 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5641 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5701 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     5761 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5821 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5881 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag
     5941 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6001 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     6061 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     6121 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     6181 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     6241 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     6301 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     6361 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6421 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6481 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc gctttaaacc cgctgatcag
     6541 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6601 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6661 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6721 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6781 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6841 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6901 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6961 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7021 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7081 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7141 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7201 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     7261 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7321 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7381 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7441 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7501 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7561 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     7621 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     7681 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     7741 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     7801 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     7861 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     7921 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     7981 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     8041 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     8101 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     8161 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     8221 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8281 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     8341 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     8401 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8461 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8521 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8581 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8821 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8881 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8941 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9001 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9061 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9121 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9181 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9241 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9301 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9361 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9421 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9481 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9541 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9601 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9661 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9721 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9781 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9841 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9901 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9961 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10021 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10081 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10141 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10201 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10261 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10321 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10381 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10441 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10501 cgcacatttc cccgaaaagt gccacctgac gtcg
//
