LOCUS       sequence-477901-       10534 bp    DNA     circular SYN 08-JUL-2025
DEFINITION  Express 3xFlag N-terminal tagged human GPR75 p.Gln234* mutant 
            transiently via transfection or stably via lentivirus-mediated 
            infection in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Jiang Y, Xun Y, Zhang Z
  TITLE     Central regulation of feeding and body weight by ciliary GPR75.
  JOURNAL   J Clin Invest. 2024 Aug 13;134(19):e182121. doi: 10.1172/JCI182121.
  PUBMED    39137039
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2024-08-13"; volume: "134(19):e182121. doi"; pages:
            " 10.1172/JCI182121"
COMMENT     Sequence Label: pBOB-3xFlag-hGpr75-p.Gln234*
FEATURES             Location/Qualifiers
     source          1..10534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3133..3142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3142..3207
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          4935..5164
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5147..5166
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5341..5929
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5394..5414)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5812..5823)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6336..6516
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6542..6559)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6548..6772
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6818..7246
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6905..6924)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7115..7136
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7255..7275)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7260..7589
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7440..7575
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7502..7521
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7637..7684
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7703..8077
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8207..8328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8244..8263)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8298..8317
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8377..8393)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8377..8393)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8390..8412)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8401..8417
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8425..8431,8432..8449,8450..8455))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8470..8491
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8608..8625)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8779..9367)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8859..8878)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9538..10329,10330..10398))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10161..10180
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10399..10503)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgat
     3181 atcgattaca aggatgacga tgacaagtct agagaattcg gatccatgaa ctcaacaggc
     3241 caccttcagg atgcccccaa tgccacctcg ctccatgtgc ctcactcaca ggaaggaaac
     3301 agcacctctc tccaggaggg tcttcaggat ctcatccaca cagccacctt ggtgacctgt
     3361 acttttctac tggcggtcat cttctgcctg ggttcctatg gcaacttcat tgtcttcttg
     3421 tccttcttcg atccagcctt caggaaattc agaaccaact ttgatttcat gatcctgaac
     3481 ctgtccttct gtgacctctt catttgtgga gtgacagccc ccatgttcac ctttgtgtta
     3541 ttcttcagct cagccagtag tatcccggat gctttctgct tcactttcca tctcaccagt
     3601 tcaggcttca tcatcatgtc tctgaagaca gtggcagtga tcgccctgca ccggctccgg
     3661 atggtgttgg ggaaacagcc taatcgcacg gcctcctttc cctgcaccgt actcctcacc
     3721 ctgcttctct gggccaccag tttcaccctt gccaccttgg ctaccttgaa aaccagcaag
     3781 tcccacctct gtcttcccat gtccagtctg attgctggaa aagggaaagc cattttgtct
     3841 ctctatgtgg tcgacttcac cttctgtgtt gctgtggtct ctgtctctta catcatgatt
     3901 gctcagaccc tgcggaagaa cgcttaagtc agaaagtgcc cccctgtaat cacagtcgat
     3961 gcttccagac cacagccttt catgggggtc cctgtgcagg gaggtggaga tcccatccag
     4021 tgtgccatgc cggctctgta taggaaccag aattacaaca aactgcagca cgttcagacc
     4081 cgtggatata ccaagagtcc caaccaactg gtcacccctg cagcaagccg actccagctc
     4141 gtatcagcca tcaacctctc cactgccaag gattccaaag ccgtggtcac ctgtgtgatc
     4201 attgtgctgt cagtcctggt gtgctgtctt ccactgggga tttccttggt acaggtggtt
     4261 ctctccagca atgggagctt cattctttac cagtttgaat tgtttggatt tactcttata
     4321 tttttcaagt caggattaaa cccttttata tattctcgga acagtgcagg gctgagaagg
     4381 aaagtgctct ggtgcctcca atacataggc ctgggttttt tctgctgcaa acaaaagact
     4441 cgacttcgag ccatgggaaa agggaacctc gaagtcaaca gaaacaaatc ctcccatcat
     4501 gaaacaaact ctgcctacat gttatctcca aagccacaga agaaatttgt ggaccaggct
     4561 tgtggcccaa gtcattcaaa agaaagtatg gtgagtccca agatctctgc tggacatcaa
     4621 cactgtggtc agagcagctc gacccccatc aacactcgga ttgaacctta ctacagcatc
     4681 tataacagca gcccttccca ggaggagagc agcccatgta acttacagcc agtaaactct
     4741 tttggatttg ccaattcata tattgccatg cattatcaca ccactaatga cttagtgcag
     4801 gaatatgaca gcacttcagc caagcagatt ccagtcccct ccgtttaact cgagccatgg
     4861 aagcttgata tctaactgac tgaaccggtg gtaccgagga attaattcgc tgtctgcgag
     4921 ggccagctgt tggggtgagt actccctctc aaaagcgggc atgacttctg cgctaagatt
     4981 gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga tgcctttgag
     5041 ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct tgaggtgtgg
     5101 caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     5161 acaggtgtcc actcccaggt ccaactgcag gtcgagcatg catctagggc ggccaattcc
     5221 gccgatcctg gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     5281 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcagac
     5341 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5401 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5461 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5521 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5581 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5641 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5701 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     5761 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5821 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5881 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
     5941 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6001 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     6061 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     6121 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     6181 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     6241 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     6301 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     6361 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6421 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6481 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc cgctttaaac ccgctgatca
     6541 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6601 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6661 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6721 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6781 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6841 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6901 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6961 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7021 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7081 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7141 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7201 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7261 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7321 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7381 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7441 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7501 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7561 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7621 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     7681 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     7741 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     7801 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     7861 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     7921 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     7981 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     8041 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     8101 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8161 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8221 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8281 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     8341 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8401 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8461 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8521 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8581 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8641 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8701 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8761 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8821 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8881 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8941 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9001 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9061 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9121 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9181 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9241 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9301 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9361 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9421 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9481 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9541 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9601 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9661 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9721 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9781 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9841 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9901 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9961 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10021 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10081 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10141 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10201 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10261 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10321 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10381 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10441 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10501 gcgcacattt ccccgaaaag tgccacctga cgtc
//
