LOCUS       sequence-477941-       12614 bp    DNA     circular SYN 09-JUL-2025
DEFINITION  Stable transformation or transient expression of gene of interest in
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12614)
  AUTHORS   Kim G, Wan W, Kim N, Yi YY, Charoennit N, Lee RRQ, Liew YY, Ng SK, 
            Zhang Y, Song J, Chae E
  TITLE     Structure determinants of DANGEROUS MIX 3, an alpha/beta hydrolase, 
            for triggering NLR-mediated genetic incompatibility in plants
  JOURNAL   bioRxiv 2024.10.28.620575; doi: 
            https://doi.org/10.1101/2024.10.28.620575
REFERENCE   2  (bases 1 to 12614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.28.620575; doi: https://doi.org/10.1101/2024.10.28.620575"
COMMENT     Sequence Label: pGWB512_35S-FLAG-(Delta-TP)DM3(Col-0)
FEATURES             Location/Qualifiers
     source          1..12614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1178..1201
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1211..1235
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3408..3432)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3443..3466
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(3486..3510)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3536..3788
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3821..3837)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3821..3837)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3834..3856)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3845..3861
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3869..3875,3876..3893,3894..3899))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3914..3935
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3971..4223)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4374..5399)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5451..5630)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6136..6160
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6681..7472
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7718..8306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8207..8226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8460..8477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8492..8632
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8558..8577)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8718..8740
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8976..9170
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9236..10309)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10738..11367)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcgac tacaaggatg acgatgacaa
     1201 ggctgttatc acaagtttgt acaaaaaagc aggctccgaa ttcgccctta tggaagcggg
     1261 atcggtttac gtcgatgtag ctggggaatc gaagtcggag catgttactg ggaagtggtt
     1321 ttcggtgccg gagctccgac tgcgtgacca ccggttcata gttcctcttg attattccaa
     1381 aagttctccc aaaattaccg tctttgctcg agagatcgtt gcgggtaact tgaaatttgg
     1441 agctttttga ttcagagctc tttctgtgat tacgaagcta aaagtttgtg tcttttgttt
     1501 tttgtagttg ggaaagagga gcaagcaatg ccgtatttat tgtatttgca aggtggtcca
     1561 ggatttgaag gtccaaggcc tagtgaagct agtggatgga ttcaaagagc ttgtgaagag
     1621 tttcgagtgg tcttactcga tcaagtatga tgctttcata gttttatact cttctctgga
     1681 cagttttttt agtctttgtg ttgttctaat tttgtttgtc ctgcagcgag gaacaggctt
     1741 gtccactcct ttaacatgtt cctctatgct tcaattcaaa tcagcaaagg aattagctga
     1801 ttatttggtt cactttcgag ctgataatat agttaaagat gctgagttta ttagagttcg
     1861 tcttgttcct aaggctgatc cttggacaat tttgggtcag gtaatttgtc tttgtattgt
     1921 ctattggtca tcttgcttca gttcttgtca tttgattttg tattcatctt tttttcttca
     1981 aatctacaga gtttcggtgg cttttgtgca cttacatatc tgagttttgc acccgaagga
     2041 ctcaaacaag tgctaataac tggaggaatt ccaccaattg gtaaagcatg tacagcagat
     2101 gatgtatatg aagccggttt tgaacaagtt gctcgccaaa atgaaaaata ctacaagaga
     2161 ttccctcaag acattgagat tgttcgtgaa ctcgtaaatt acttggctga atctgaaggt
     2221 ggcggggtga gggattacaa catttgctca atccactgac cagtttagtt tagttcctta
     2281 cttgtctctg tttctttgtc tttaggtacc tctcccatca ggaggcattc ttactcctaa
     2341 gggtcttcaa actcttggtc tttctggttt aggatcaagt actggttttg agcgcctgca
     2401 ttatatgtaa gagtttagct taatttttat caaccacctc acatgttctg tgttaaatct
     2461 ggccttctta attgatcata ggttggagag agtatgggat cctattttag ttactggagc
     2521 tccaaagtgt attagtcagt tcttcttaaa cgctgtaaga gacccacaaa aatttgttgt
     2581 tttctgttct cagctcaaat aaggaattca atagtctgag acattttatc gttgctgcag
     2641 tttgagagtt ggcattcttt tgatacaaat ccactatatg ctcttctcca cgaggccata
     2701 tactgtgagg tatagtctct tcaaatttct catctttttt tctatttcag acaattactt
     2761 cttcttgtta tcgtttatgt ttaaatggaa actttttcag ggtgcttcat caggatggtc
     2821 tgctcataga ttacgggata aatatgagta caaattcgac gcaatgaagg cagtcaaaga
     2881 aagtcagcct gtactcttca caggagaggt acaggttttg agtatttcta attgtatggc
     2941 tcatagcttg gatgattggt ccacattgct taactaaaat agaatttgga tttgtataga
     3001 tgatatttcc atggatgttt gatgaaattc atgccttgaa gccatttaaa gctgccgccg
     3061 atcttttggc caagaaagag gattggcctc cattatatga tgttcctaga ttgcagaata
     3121 acaaggtctg tttcagaatc ttgatcgttc tttcatatgg atcaaataat tttactctgt
     3181 ttcacatgat ttctttttgt aggtgcctgt tgctgcagcg gtttactacg aagatatgta
     3241 tgtaaacttc aagctggtga cggaaacagc ttctcatata tcgggtatca gactttgggt
     3301 aacgaatgag tttatgcatt cgggtcttcg tgatgctgga agacaaatta ttgatcattt
     3361 gttgggaatg atcaatggga aaaagcctct cttttagggc gaattcgacc cagctttctt
     3421 gtacaaagtg gttgataaca gcgactacaa ggatgacgat gacaaggctt agaagggcga
     3481 attcgaccca gctttcttgt acaaagtggt gataacctag agctcgaatt tccccgatcg
     3541 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     3601 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     3661 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     3721 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     3781 actagatcgg gaattggttc cggaaccaat tcgtaatcat gtcatagctg tttcctgtgt
     3841 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3901 cctggggtgc ctaatgagtg agctaactca cattaggctg aattaggcgc gcctatttct
     3961 attaattccc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
     4021 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
     4081 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
     4141 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
     4201 tattgccaaa tgtttgaacg atcggggaaa ttcgggggat cgatcccgcg aactgtggac
     4261 gagaactgtg ccaccaagcg taaggccgtt ctctcgcatt tgccttgcta ggctcgcgcg
     4321 agttgctggc tgaggcgttc tcgaaatcag ctcttgttcg gtcggcatct actctattcc
     4381 tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag
     4441 ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct
     4501 ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg
     4561 tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc
     4621 ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa
     4681 gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc
     4741 gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag
     4801 ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc
     4861 tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga
     4921 tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt
     4981 ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca
     5041 tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc
     5101 aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct gaatgcccca
     5161 atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga
     5221 tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca
     5281 tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg
     5341 tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata
     5401 tcttattgcc ccccgggatc agatctggat tgagagtgaa tatgagactc taattggata
     5461 ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga
     5521 tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc
     5581 attaaactcc agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc
     5641 aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc
     5701 tccgctcatg atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
     5761 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
     5821 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc
     5881 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
     5941 cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc
     6001 ttgcgccctg agtgcttgcg gcagcgtgaa gctgatccgt cgagattaat agaaataaat
     6061 tcagcctaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     6121 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     6181 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     6241 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     6301 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     6361 tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga
     6421 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     6481 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     6541 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     6601 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     6661 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     6721 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     6781 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     6841 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     6901 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     6961 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     7021 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     7081 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     7141 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     7201 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     7261 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     7321 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     7381 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     7441 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     7501 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     7561 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
     7621 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact
     7681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7861 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     8521 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     8581 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     8641 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     8701 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     8761 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt
     8821 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg
     8881 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
     8941 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
     9001 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
     9061 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
     9121 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac
     9181 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
     9241 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
     9301 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
     9361 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
     9421 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
     9481 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
     9541 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
     9601 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
     9661 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
     9721 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
     9781 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
     9841 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
     9901 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
     9961 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
    10021 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
    10081 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc
    10141 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg
    10201 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac
    10261 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc
    10321 gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg
    10381 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg
    10441 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca
    10501 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc
    10561 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct
    10621 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg
    10681 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca
    10741 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc
    10801 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg
    10861 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc
    10921 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc
    10981 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta
    11041 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa
    11101 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac
    11161 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac
    11221 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc
    11281 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt
    11341 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat
    11401 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt
    11461 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg
    11521 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc
    11581 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt
    11641 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg
    11701 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct
    11761 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc
    11821 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt
    11881 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg
    11941 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc
    12001 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg
    12061 ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc
    12121 gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt
    12181 ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa
    12241 acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt
    12301 cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat
    12361 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac
    12421 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt
    12481 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt
    12541 ggatcaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac
    12601 gaacggataa acct
//
