LOCUS       sequence-477948-       12539 bp    DNA     circular SYN 09-JUL-2025
DEFINITION  Stable transformation or transient expression of gene of interest in
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12539)
  AUTHORS   Kim G, Wan W, Kim N, Yi YY, Charoennit N, Lee RRQ, Liew YY, Ng SK, 
            Zhang Y, Song J, Chae E
  TITLE     Structure determinants of DANGEROUS MIX 3, an alpha/beta hydrolase, 
            for triggering NLR-mediated genetic incompatibility in plants
  JOURNAL   bioRxiv 2024.10.28.620575; doi: 
            https://doi.org/10.1101/2024.10.28.620575
REFERENCE   2  (bases 1 to 12539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.28.620575; doi: https://doi.org/10.1101/2024.10.28.620575"
COMMENT     Sequence Label: pGWB512_35S-FLAG-(Delta-TP)DM3(Col-0) Delta-C
FEATURES             Location/Qualifiers
     source          1..12539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1178..1201
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1211..1235
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3411..3435)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3461..3713
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3746..3762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3746..3762)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3759..3781)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3770..3786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3794..3800,3801..3818,3819..3824))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3839..3860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3896..4148)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4299..5324)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5376..5555)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6061..6085
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6606..7397
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7643..8231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8132..8151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8385..8402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8417..8557
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8483..8502)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8643..8665
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8901..9095
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9161..10234)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10663..11292)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcgac tacaaggatg acgatgacaa
     1201 ggctgttatc acaagtttgt acaaaaaagc aggctttcga attccaatgg aagcgggatc
     1261 ggtttacgtc gatgtagctg gggaatcgaa gtcggagcat gttactggga agtggttttc
     1321 ggtgccggag ctccgactgc gtgaccaccg gttcatagtt cctcttgatt attccaaaag
     1381 ttctcccaaa attaccgtct ttgctcgaga gatcgttgcg ggtaacttga aatttggagc
     1441 tttttgattc agagctcttt ctgtgattac gaagctaaaa gtttgtgtct tttgtttttt
     1501 gtagttggga aagaggagca agcaatgccg tatttattgt atttgcaagg tggtccagga
     1561 tttgaaggtc caaggcctag tgaagctagt ggatggattc aaagagcttg tgaagagttt
     1621 cgagtggtct tactcgatca agtatgatgc tttcatagtt ttatactctt ctctggacag
     1681 tttttttagt ctttgtgttg ttctaatttt gtttgtcctg cagcgaggaa caggcttgtc
     1741 cactccttta acatgttcct ctatgcttca attcaaatca gcaaaggaat tagctgatta
     1801 tttggttcac tttcgagctg ataatatagt taaagatgct gagtttatta gagttcgtct
     1861 tgttcctaag gctgatcctt ggacaatttt gggtcaggta atttgtcttt gtattgtcta
     1921 ttggtcatct tgcttcagtt cttgtcattt gattttgtat tcatcttttt ttcttcaaat
     1981 ctacagagtt tcggtggctt ttgtgcactt acatatctga gttttgcacc cgaaggactc
     2041 aaacaagtgc taataactgg aggaattcca ccaattggta aagcatgtac agcagatgat
     2101 gtatatgaag ccggttttga acaagttgct cgccaaaatg aaaaatacta caagagattc
     2161 cctcaagaca ttgagattgt tcgtgaactc gtaaattact tggctgaatc tgaaggtggc
     2221 ggggtgaggg attacaacat ttgctcaatc cactgaccag tttagtttag ttccttactt
     2281 gtctctgttt ctttgtcttt aggtacctct cccatcagga ggcattctta ctcctaaggg
     2341 tcttcaaact cttggtcttt ctggtttagg atcaagtact ggttttgagc gcctgcatta
     2401 tatgtaagag tttagcttaa tttttatcaa ccacctcaca tgttctgtgt taaatctggc
     2461 cttcttaatt gatcataggt tggagagagt atgggatcct attttagtta ctggagctcc
     2521 aaagtgtatt agtcagttct tcttaaacgc tgtaagagac ccacaaaaat ttgttgtttt
     2581 ctgttctcag ctcaaataag gaattcaata gtctgagaca ttttatcgtt gctgcagttt
     2641 gagagttggc attcttttga tacaaatcca ctatatgctc ttctccacga ggccatatac
     2701 tgtgaggtat agtctcttca aatttctcat ctttttttct atttcagaca attacttctt
     2761 cttgttatcg tttatgttta aatggaaact ttttcagggt gcttcatcag gatggtctgc
     2821 tcatagatta cgggataaat atgagtacaa attcgacgca atgaaggcag tcaaagaaag
     2881 tcagcctgta ctcttcacag gagaggtaca ggttttgagt atttctaatt gtatggctca
     2941 tagcttggat gattggtcca cattgcttaa ctaaaataga atttggattt gtatagatga
     3001 tatttccatg gatgtttgat gaaattcatg ccttgaagcc atttaaagct gccgccgatc
     3061 ttttggccaa gaaagaggat tggcctccat tatatgatgt tcctagattg cagaataaca
     3121 aggtctgttt cagaatcttg atcgttcttt catatggatc aaataatttt actctgtttc
     3181 acatgatttc tttttgtagg tgcctgttgc tgcagcggtt tactacgaag atatgtatgt
     3241 aaacttcaag ctggtgacgg aaacagcttc tcatatatcg ggtatcagac tttgggtaac
     3301 gaatgagttt atgcattcgg gtcttcgtga tgctggaaga caaattattg atcatttgtt
     3361 gggaatgatc aatgggtgaa agcctctctt ttgaccgaat tcctatctag acccagcttt
     3421 cttgtacaaa gtggtgataa cctagagctc gaatttcccc gatcgttcaa acatttggca
     3481 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     3541 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     3601 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     3661 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     3721 ggttccggaa ccaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
     3781 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3841 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctattaa ttcccgatct
     3901 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     3961 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     4021 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     4081 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     4141 gaacgatcgg ggaaattcgg gggatcgatc ccgcgaactg tggacgagaa ctgtgccacc
     4201 aagcgtaagg ccgttctctc gcatttgcct tgctaggctc gcgcgagttg ctggctgagg
     4261 cgttctcgaa atcagctctt gttcggtcgg catctactct attcctttgc cctcggacga
     4321 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg
     4381 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt
     4441 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga
     4501 tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc
     4561 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc
     4621 cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca
     4681 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc
     4741 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc
     4801 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
     4861 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
     4921 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg
     4981 accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
     5041 gcacggcggg agatgcaata ggtcaggctc tcgctgaatg ccccaatgtc aagcacttcc
     5101 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
     5161 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
     5221 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc
     5281 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatctta ttgccccccg
     5341 ggatcagatc tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     5401 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     5461 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     5521 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     5581 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatctt
     5641 gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc
     5701 ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa
     5761 accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg
     5821 cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt
     5881 ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc
     5941 ttgcggcagc gtgaagctga tccgtcgaga ttaatagaaa taaattcagc ctaattcggc
     6001 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     6061 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     6121 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     6181 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     6241 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     6301 cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg
     6361 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     6421 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     6481 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     6541 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     6601 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     6661 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     6721 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     6781 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     6841 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     6901 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     6961 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     7021 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     7081 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     7141 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     7201 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     7261 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     7321 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     7381 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
     7441 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     7501 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     7561 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7621 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7681 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7741 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7801 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7861 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7921 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7981 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8041 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8101 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8161 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8221 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8281 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8341 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8401 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     8461 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     8521 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     8581 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     8641 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     8701 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     8761 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     8821 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     8881 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     8941 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     9001 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     9061 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     9121 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     9181 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     9241 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     9301 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     9361 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     9421 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     9481 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     9541 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     9601 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     9661 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     9721 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     9781 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     9841 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
     9901 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
     9961 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
    10021 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
    10081 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
    10141 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
    10201 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
    10261 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
    10321 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
    10381 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
    10441 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
    10501 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
    10561 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
    10621 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
    10681 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
    10741 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    10801 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    10861 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    10921 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    10981 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    11041 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    11101 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    11161 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    11221 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    11281 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    11341 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    11401 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    11461 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    11521 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    11581 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    11641 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    11701 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    11761 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    11821 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    11881 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    11941 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    12001 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    12061 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    12121 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    12181 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    12241 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    12301 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    12361 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    12421 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    12481 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
