LOCUS       sequence-477950-        8559 bp    DNA     circular SYN 09-JUL-2025
DEFINITION  Expression of hhN-HA under control of UAS promoter. Can be used to 
            generate transgenic fly lines by white+ fly eye selection and phiC31
            integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8559)
  TITLE     Perrimon Lab Plasmids 2025
REFERENCE   2  (bases 1 to 8559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUAS-hhN-HA
FEATURES             Location/Qualifiers
     source          1..8559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(840..957,1034..1127,1219..1825,1880..2013,
                     2074..2234,2308..2333))
                     /codon_start=1
                     /product="tryptophan oxygenase"
                     /label=vermilion
                     /note="selectable marker, required to synthesize the brown 
                     eye pigment in Drosophila"
                     /translation="MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSE
                     EDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVL
                     ILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKY
                     SDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQV
                     QSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITF
                     YRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTL
                     SDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV"
     protein_bind    2648..2717
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    2955..3385
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    3403..3436
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3449..3543
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3559..3592
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3605..3699
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3723..3961
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3908..3929
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    4006..4030
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4849..4875
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4894..4941
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     protein_bind    complement(4964..4988)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5295..5360
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5490..5510
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5782..5903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5819..5838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5873..5892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    5925..6355
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(6374..6394)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6374..6392)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6413..6429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6413..6429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6426..6448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6437..6453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6461..6467,6468..6485,6486..6491))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6506..6527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6644..6661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6815..7403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6895..6914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7574..8365,8366..8434))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8197..8216
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8435..8539)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaaatgagct gatttaacaa
      421 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caagcttgga tttattttgt tatgttatat gtattatatg tcagacataa agaaaaggaa
      721 cacatcaaat gtgataacaa agactaaaca agtaatttta ttacaccaaa acgacaaaac
      781 agtaggcaga acaaacaacg catagccaaa cattgacgaa ttggataccc tgccgattgt
      841 cagacacttt tgttgatcag tttcttgcga atggtctcgt ccagcggtgg aatcgcctcg
      901 cggggaatca gaaaagtgga cagattgaac agatccagaa acaccttgta ccgatcactg
      961 aaaccaaaaa aaaacaaagg gagaacagtt tgagttcatt gatccccgat ataatcacat
     1021 ctgcgatgat cacctgagag tggagcgcag atattgatat ccagacgagc caccagtgcc
     1081 caactgttgg gatccaatca tgcgttgcac catgatcacg tgattgtctg cggcgggaat
     1141 agaaagtatt tggttaggaa aaccagtctt aaacataaga tatatttata aaagagtatc
     1201 aaagaatgca atacttacat ctccacttgg ttattaacga gtcgatgtcc atgagcaggg
     1261 tgagcaactg gtgtggttgg ctgaacctgg gttcatccct atagaaggtg atcatgatgg
     1321 ctccctgaag ggcacgatgg ctaaaccggc gatccccacg acgcaccagt gcatcgtgca
     1381 ctgccggatc aaagatggag cgatacacct cgcgtcgctt ctcaatgtcc atgaggcggt
     1441 agtttttcgc cttctccacg ggctcctcca tggcgctctg tacctgcgcc tccaggaatc
     1501 gatcgacgct ctcctgaaac ttggcccaga agttgaagcc actctcctcc agtccgggcg
     1561 tcctctccag ccatcgctgc actagctcca gtagcgaggg atctttctcc gagttgcgaa
     1621 tcgagttccg cgcctcctcg tcgctaaaga catccgagta cttctggttg tatctcaccc
     1681 gctgctctgt cagaactccc agcttgttct cgatcaaacg gaactgcagc gactgaaaac
     1741 cagatgcggg tgccaggtac ttgcggaagt ccatgaagtc tagcggggtc atggtctcca
     1801 gaatgggcac ttggtccacc aggagctgta caaaggaagt tataaacgga ttttggtaag
     1861 agattcagaa agcactcact tttagaatca gaaccactcg gttcagtcgc ttgacaatct
     1921 ccagcgtctt ggtttcatcg atgacctctg catccaacat gtctcgtatg gagtcgaact
     1981 caaagatgat ctgcttgaac caaagctcgt aggctgtggc gaaggtactt aaatgccatt
     2041 gagtgttgtc atcaaagttg taaacctact caccctggtg cgtgatgatg aacagatgct
     2101 catcgtgcac gggtcgcttg tcctcctcgg acagcataca ctgggcatcc agcagtttgt
     2161 ccagcatcag atactctcca tagattttgc ccacttccgt ggttaatggc accgccgaat
     2221 catcgtgatc gtttctgtat gggtttgaat tgaatcgcag aactgaagat cgattggcat
     2281 tcctggacag cacgtgctgg tgctcacccg tttcctgcat agggacagct catggtgcac
     2341 agctcagatc agatcgtgac tcctcgagcg gcggatgctg gcgaactgat ctccgccagc
     2401 ggaccggaga tgagacccca gcgaaccgat aacagagcga gagagctcca gttccgactg
     2461 attgcacagt cggtgatctg ggcgatgggc actgccagat aggctgggaa ttatcaatca
     2521 cttgaggtga aagtgcggcg cacacaaatc caagcttgat atcatcgatc tcgaggctgc
     2581 atccaacgcg ttgggagctc tccggatcaa ttcggcttca ggtaccgtcg acgatgtagg
     2641 tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt
     2701 actccacctc acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc
     2761 cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca
     2821 cggtgagcac gggacgtgcg acggcgtcgg ctggtgcgga tacgcggggc agcgtcagcg
     2881 ggttctcgac ggtcacggcg ggcatgtcga caagccgaat tgatccacta gaaggcctaa
     2941 ttcggtacac tagttggcca cgtaataagt gtgcgttgaa tttattcgca aaaacattgc
     3001 atattttcgg caaagtaaaa ttttgttgca taccttatca aaaaataagt gctgcatact
     3061 ttttagagaa accaaataat tttttattgc atacccgttt ttaataaaat acattgcata
     3121 ccctctttta ataaaaaata ttgcatactt tgacgaaaca aattttcgtt gcatacccaa
     3181 taaaagatta ttatattgca tacccgtttt taataaaata cattgcatac cctcttttaa
     3241 taaagaatat tgcatacgtt gacgaaacaa attttcgttg catacccaat aaaagattat
     3301 tatattgcat accttttctt gccataccat ttagccgatc aattgtgctc ggcaacagta
     3361 tatttgtggt gtgccaacca acaacactag tagtaccagc ttataacttc gtataatgta
     3421 tgctatacga agttatctgc aggcaggtcg gagtactgtc ctccgagcgg agtactgtcc
     3481 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
     3541 gagcggagac tcccatggat aacttcgtat aatgtatgct atacgaagtt atggatccgc
     3601 aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
     3661 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcgt cgacagcgag
     3721 cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa
     3781 agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa
     3841 caatctgcag taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa
     3901 atcaactgca actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct
     3961 gaatagggaa ttgggaattc cagatctgcg gccgcggctc gaatcacaag tttgtacaaa
     4021 aaagcaggct ccgcggccgc ccccttcacc atggataacc acagctcagt gccttgggcc
     4081 agtgccgcca gtgtcacctg tctctccctg gatgccaaat gccacagttc cagttccagt
     4141 tccagctcca aatccgcagc gagctccatc tccgcaatcc cgcaagaaga aacgcaaacg
     4201 atgcgccaca ttgcgcatac gcagcgttgc ctcagcaggc tgacctctct ggtggccctg
     4261 ctgctgatcg tcttgccgat ggtctttagc ccggctcaca gctgcggtcc tggccgagga
     4321 ttgggtcgtc atagggcgcg caacctgtat ccgctggtcc tcaagcagac aattcccaat
     4381 ctatccgagt acacgaacag cgcctccgga cctctggagg gtgtgatccg tcgggactcg
     4441 cccaaattca aggacctcgt gcccaactac aacagggaca tccttttccg cgacgaggaa
     4501 ggcaccggag cggatcgctt gatgagcaag cgctgcaagg agaagctaaa cgtgctggcc
     4561 tactcggtga tgaacgaatg gcccggcatc cggctgctgg tcaccgagag ctgggacgag
     4621 gactaccatc acggccagga gtcgctccac tacgagggcc gagcggtgac cattgccacc
     4681 tccgatcgcg accagtccaa atacggcatg ctcgctcgcc tggccgtcga ggctggattc
     4741 gattgggtct cctacgtcag caggcgccac atctactgct ccgtcaagtc agattcgtcg
     4801 atcagttccc acgtgcacgg cggtgatggt ggctcgggtg gaggaagtta cccatacgat
     4861 gttccagatt acgctggagg ctccggtact agccgtgacc acatggtcct tcatgagtat
     4921 gtaaatgctg ctgggattac ataatagaag ggtgggcgcg ccgacccagc tttcttgtac
     4981 aaagtggtga ttcgagggta cctctagagc aaactagttc tgatctgcta gacaattgtt
     5041 ggcatcaggt aggcatcaca cacgattaac aacccctaaa aatacacttt gaaaatattg
     5101 aaaatatgtt tttgtataca tttttgatat tttcaaataa tacgcagtta taaaactcat
     5161 tagctaaccc attttttctt tgcttatgct tacagattgc aaagaactag agccgcggga
     5221 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     5281 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     5341 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     5401 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     5461 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     5521 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     5581 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     5641 tggaaaaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     5701 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     5761 atgaatggaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     5821 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5881 tccaaactca tcaatgtatc ttatcatgtc tggttccaga gctctggcca cgtaataagt
     5941 gtgcgttgaa tttattcgca aaaacattgc atattttcgg caaagtaaaa ttttgttgca
     6001 taccttatca aaaaataagt gctgcatact ttttagagaa accaaataat tttttattgc
     6061 atacccgttt ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatactt
     6121 tgacgaaaca aattttcgtt gcatacccaa taaaagatta ttatattgca tacccgtttt
     6181 taataaaata cattgcatac cctcttttaa taaagaatat tgcatacgtt gacgaaacaa
     6241 attttcgttg catacccaat aaaagattat tatattgcat accttttctt gccataccat
     6301 ttagccgatc aattgtgctc ggcaacagta tatttgtggt gtgccaacca acaacgagct
     6361 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     6421 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6481 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6541 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6601 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6661 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6721 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6781 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6841 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6901 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6961 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7021 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7081 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7141 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7201 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7261 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7321 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7381 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7441 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7501 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7561 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7621 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7681 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7741 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7801 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7861 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7921 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7981 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8041 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8101 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8161 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8221 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8281 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8341 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8401 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8461 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8521 aacaaatagg ggttccgcgc acatttcccc gaaaagtgc
//
