LOCUS       sequence-477951-        8688 bp    DNA     circular SYN 09-JUL-2025
DEFINITION  Expression of spatzle-HA under control of UAS promoter. Can be used 
            to generate transgenic fly lines by white+ fly eye selection and 
            phiC31 integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8688)
  TITLE     Perrimon Lab Plasmids 2025
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUAS-spatzle-HA
FEATURES             Location/Qualifiers
     source          1..8688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(840..957,1034..1127,1219..1825,1880..2013,
                     2074..2234,2308..2333))
                     /codon_start=1
                     /product="tryptophan oxygenase"
                     /label=vermilion
                     /note="selectable marker, required to synthesize the brown 
                     eye pigment in Drosophila"
                     /translation="MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSE
                     EDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVL
                     ILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKY
                     SDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQV
                     QSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITF
                     YRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTL
                     SDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV"
     protein_bind    2648..2717
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    2955..3385
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    3403..3436
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3449..3543
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3559..3592
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3605..3699
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3723..3961
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3908..3929
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    4006..4030
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4978..5004
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5023..5070
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     protein_bind    complement(5093..5117)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5424..5489
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5619..5639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5911..6032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5948..5967)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6002..6021
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6054..6484
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(6503..6523)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6503..6521)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6542..6558)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6542..6558)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6555..6577)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6566..6582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6590..6596,6597..6614,6615..6620))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6635..6656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6773..6790)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6944..7532)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7024..7043)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7703..8494,8495..8563))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8326..8345
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8564..8668)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaaatgagct gatttaacaa
      421 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caagcttgga tttattttgt tatgttatat gtattatatg tcagacataa agaaaaggaa
      721 cacatcaaat gtgataacaa agactaaaca agtaatttta ttacaccaaa acgacaaaac
      781 agtaggcaga acaaacaacg catagccaaa cattgacgaa ttggataccc tgccgattgt
      841 cagacacttt tgttgatcag tttcttgcga atggtctcgt ccagcggtgg aatcgcctcg
      901 cggggaatca gaaaagtgga cagattgaac agatccagaa acaccttgta ccgatcactg
      961 aaaccaaaaa aaaacaaagg gagaacagtt tgagttcatt gatccccgat ataatcacat
     1021 ctgcgatgat cacctgagag tggagcgcag atattgatat ccagacgagc caccagtgcc
     1081 caactgttgg gatccaatca tgcgttgcac catgatcacg tgattgtctg cggcgggaat
     1141 agaaagtatt tggttaggaa aaccagtctt aaacataaga tatatttata aaagagtatc
     1201 aaagaatgca atacttacat ctccacttgg ttattaacga gtcgatgtcc atgagcaggg
     1261 tgagcaactg gtgtggttgg ctgaacctgg gttcatccct atagaaggtg atcatgatgg
     1321 ctccctgaag ggcacgatgg ctaaaccggc gatccccacg acgcaccagt gcatcgtgca
     1381 ctgccggatc aaagatggag cgatacacct cgcgtcgctt ctcaatgtcc atgaggcggt
     1441 agtttttcgc cttctccacg ggctcctcca tggcgctctg tacctgcgcc tccaggaatc
     1501 gatcgacgct ctcctgaaac ttggcccaga agttgaagcc actctcctcc agtccgggcg
     1561 tcctctccag ccatcgctgc actagctcca gtagcgaggg atctttctcc gagttgcgaa
     1621 tcgagttccg cgcctcctcg tcgctaaaga catccgagta cttctggttg tatctcaccc
     1681 gctgctctgt cagaactccc agcttgttct cgatcaaacg gaactgcagc gactgaaaac
     1741 cagatgcggg tgccaggtac ttgcggaagt ccatgaagtc tagcggggtc atggtctcca
     1801 gaatgggcac ttggtccacc aggagctgta caaaggaagt tataaacgga ttttggtaag
     1861 agattcagaa agcactcact tttagaatca gaaccactcg gttcagtcgc ttgacaatct
     1921 ccagcgtctt ggtttcatcg atgacctctg catccaacat gtctcgtatg gagtcgaact
     1981 caaagatgat ctgcttgaac caaagctcgt aggctgtggc gaaggtactt aaatgccatt
     2041 gagtgttgtc atcaaagttg taaacctact caccctggtg cgtgatgatg aacagatgct
     2101 catcgtgcac gggtcgcttg tcctcctcgg acagcataca ctgggcatcc agcagtttgt
     2161 ccagcatcag atactctcca tagattttgc ccacttccgt ggttaatggc accgccgaat
     2221 catcgtgatc gtttctgtat gggtttgaat tgaatcgcag aactgaagat cgattggcat
     2281 tcctggacag cacgtgctgg tgctcacccg tttcctgcat agggacagct catggtgcac
     2341 agctcagatc agatcgtgac tcctcgagcg gcggatgctg gcgaactgat ctccgccagc
     2401 ggaccggaga tgagacccca gcgaaccgat aacagagcga gagagctcca gttccgactg
     2461 attgcacagt cggtgatctg ggcgatgggc actgccagat aggctgggaa ttatcaatca
     2521 cttgaggtga aagtgcggcg cacacaaatc caagcttgat atcatcgatc tcgaggctgc
     2581 atccaacgcg ttgggagctc tccggatcaa ttcggcttca ggtaccgtcg acgatgtagg
     2641 tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt
     2701 actccacctc acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc
     2761 cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca
     2821 cggtgagcac gggacgtgcg acggcgtcgg ctggtgcgga tacgcggggc agcgtcagcg
     2881 ggttctcgac ggtcacggcg ggcatgtcga caagccgaat tgatccacta gaaggcctaa
     2941 ttcggtacac tagttggcca cgtaataagt gtgcgttgaa tttattcgca aaaacattgc
     3001 atattttcgg caaagtaaaa ttttgttgca taccttatca aaaaataagt gctgcatact
     3061 ttttagagaa accaaataat tttttattgc atacccgttt ttaataaaat acattgcata
     3121 ccctctttta ataaaaaata ttgcatactt tgacgaaaca aattttcgtt gcatacccaa
     3181 taaaagatta ttatattgca tacccgtttt taataaaata cattgcatac cctcttttaa
     3241 taaagaatat tgcatacgtt gacgaaacaa attttcgttg catacccaat aaaagattat
     3301 tatattgcat accttttctt gccataccat ttagccgatc aattgtgctc ggcaacagta
     3361 tatttgtggt gtgccaacca acaacactag tagtaccagc ttataacttc gtataatgta
     3421 tgctatacga agttatctgc aggcaggtcg gagtactgtc ctccgagcgg agtactgtcc
     3481 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
     3541 gagcggagac tcccatggat aacttcgtat aatgtatgct atacgaagtt atggatccgc
     3601 aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
     3661 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcgt cgacagcgag
     3721 cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa
     3781 agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa
     3841 caatctgcag taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa
     3901 atcaactgca actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct
     3961 gaatagggaa ttgggaattc cagatctgcg gccgcggctc gaatcacaag tttgtacaaa
     4021 aaagcaggct ccgcggccgc ccccttcacc atgatgacgc ccatgtggat atcgctgttc
     4081 aaagtcctgc tgctgctgtt cgccttcttt gccacgacgt ccgcggacag tgcacccttc
     4141 atgcccatac ccacccagca cgacgatccg acccagaaac agaaacagaa tcagaatcaa
     4201 tctccgatac ccgaaacgaa ccgccattac catcagtatc acagcctgat ccaaccagat
     4261 cagtacttca aagtgcagcg ctccccgaat ggaaagctga atctggtctt taacgatacg
     4321 ttcgtatctt tgcagaggac ggacacggag gtgcaatccg aacagccgat acccccccga
     4381 catccatcgg atacctttgt cttccccgat tcccccatag ccaagtatcg gccaccacaa
     4441 tccccagctc gtcctttgag gaatgacact aaggagcaca atccctgcgc caaggatgaa
     4501 agccaacacc ttagaaactt ctgcacgaat gtggacgact atccggacct ttcaggcctc
     4561 acacataagc taaaaaacaa cttcgccaag ttctttagca acgatcttca gcccacggat
     4621 gtgagctctc gcgttggtgg ctcagatgag cgattccttt gcaggagcat caggaagctg
     4681 gtgtacccaa aaaagggctt gagggcggac gacacctggc agttaattgt caataacgat
     4741 gagtacaaac aggccatcca gatcgaagag tgcgaaggag cggatcaacc ctgtgacttt
     4801 gccgccaact ttccgcagag ctataatcca atctgcaagc agcactacac acagcagacc
     4861 ctggccagca tcaagagtga tggcgaactg gacgtggtgc agaattcctt caagatcccc
     4921 tcctgctgca agtgcgcgtt gaagactggg ggtgatggtg gctcgggtgg aggaagttac
     4981 ccatacgatg ttccagatta cgctggaggc tccggtacta gccgtgacca catggtcctt
     5041 catgagtatg taaatgctgc tgggattaca taatagaagg gtgggcgcgc cgacccagct
     5101 ttcttgtaca aagtggtgat tcgagggtac ctctagagca aactagttct gatctgctag
     5161 acaattgttg gcatcaggta ggcatcacac acgattaaca acccctaaaa atacactttg
     5221 aaaatattga aaatatgttt ttgtatacat ttttgatatt ttcaaataat acgcagttat
     5281 aaaactcatt agctaaccca ttttttcttt gcttatgctt acagattgca aagaactaga
     5341 gccgcgggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     5401 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     5461 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     5521 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     5581 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     5641 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     5701 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     5761 agaaaattat ggaaaaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
     5821 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     5881 aaacataaaa tgaatggaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     5941 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6001 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggttccagag ctctggccac
     6061 gtaataagtg tgcgttgaat ttattcgcaa aaacattgca tattttcggc aaagtaaaat
     6121 tttgttgcat accttatcaa aaaataagtg ctgcatactt tttagagaaa ccaaataatt
     6181 ttttattgca tacccgtttt taataaaata cattgcatac cctcttttaa taaaaaatat
     6241 tgcatacttt gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat
     6301 acccgttttt aataaaatac attgcatacc ctcttttaat aaagaatatt gcatacgttg
     6361 acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata ccttttcttg
     6421 ccataccatt tagccgatca attgtgctcg gcaacagtat atttgtggtg tgccaaccaa
     6481 caacgagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     6541 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6601 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6661 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6721 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6781 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6841 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6901 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6961 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7021 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7081 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7141 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7201 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7261 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7321 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7381 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7441 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7501 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7561 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7621 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7681 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7741 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7801 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7861 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7921 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7981 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8041 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8101 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8161 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8221 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8281 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8341 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8401 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8461 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8521 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8581 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8641 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgc
//
