LOCUS       sequence-477960-       12479 bp    DNA     circular SYN 09-JUL-2025
DEFINITION  Stable transformation or transient expression of gene of interest in
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12479)
  AUTHORS   Kim G, Wan W, Kim N, Yi YY, Charoennit N, Lee RRQ, Liew YY, Ng SK, 
            Zhang Y, Song J, Chae E
  TITLE     Structure determinants of DANGEROUS MIX 3, an alpha/beta hydrolase, 
            for triggering NLR-mediated genetic incompatibility in plants
  JOURNAL   bioRxiv 2024.10.28.620575; doi: 
            https://doi.org/10.1101/2024.10.28.620575
REFERENCE   2  (bases 1 to 12479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.28.620575; doi: https://doi.org/10.1101/2024.10.28.620575"
COMMENT     Sequence Label: pGWB512_35S-FLAG-DM3(Col-0)68-510
FEATURES             Location/Qualifiers
     source          1..12479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1178..1201
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1211..1235
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3351..3375)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3401..3653
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3686..3702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3686..3702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3699..3721)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3710..3726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3734..3740,3741..3758,3759..3764))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3779..3800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3836..4088)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4239..5264)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5316..5495)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6001..6025
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6546..7337
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7583..8171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8072..8091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8325..8342
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8357..8497
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8423..8442)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8583..8605
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8841..9035
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9101..10174)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10603..11232)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcgac tacaaggatg acgatgacaa
     1201 ggctgttatc acaagtttgt acaaaaaagc aggctttcga attccaatgg agcatgttac
     1261 tgggaagtgg ttttcggtgc cggagctccg actgcgtgac caccggttca tagttcctct
     1321 tgattattcc aaaagttctc ccaaaattac cgtctttgct cgagagatcg ttgcgggtaa
     1381 cttgaaattt ggagcttttt gattcagagc tctttctgtg attacgaagc taaaagtttg
     1441 tgtcttttgt tttttgtagt tgggaaagag gagcaagcaa tgccgtattt attgtatttg
     1501 caaggtggtc caggatttga aggtccaagg cctagtgaag ctagtggatg gattcaaaga
     1561 gcttgtgaag agtttcgagt ggtcttactc gatcaagtat gatgctttca tagttttata
     1621 ctcttctctg gacagttttt ttagtctttg tgttgttcta attttgtttg tcctgcagcg
     1681 aggaacaggc ttgtccactc ctttaacatg ttcctctatg cttcaattca aatcagcaaa
     1741 ggaattagct gattatttgg ttcactttcg agctgataat atagttaaag atgctgagtt
     1801 tattagagtt cgtcttgttc ctaaggctga tccttggaca attttgggtc aggtaatttg
     1861 tctttgtatt gtctattggt catcttgctt cagttcttgt catttgattt tgtattcatc
     1921 tttttttctt caaatctaca gagtttcggt ggcttttgtg cacttacata tctgagtttt
     1981 gcacccgaag gactcaaaca agtgctaata actggaggaa ttccaccaat tggtaaagca
     2041 tgtacagcag atgatgtata tgaagccggt tttgaacaag ttgctcgcca aaatgaaaaa
     2101 tactacaaga gattccctca agacattgag attgttcgtg aactcgtaaa ttacttggct
     2161 gaatctgaag gtggcggggt gagggattac aacatttgct caatccactg accagtttag
     2221 tttagttcct tacttgtctc tgtttctttg tctttaggta cctctcccat caggaggcat
     2281 tcttactcct aagggtcttc aaactcttgg tctttctggt ttaggatcaa gtactggttt
     2341 tgagcgcctg cattatatgt aagagtttag cttaattttt atcaaccacc tcacatgttc
     2401 tgtgttaaat ctggccttct taattgatca taggttggag agagtatggg atcctatttt
     2461 agttactgga gctccaaagt gtattagtca gttcttctta aacgctgtaa gagacccaca
     2521 aaaatttgtt gttttctgtt ctcagctcaa ataaggaatt caatagtctg agacatttta
     2581 tcgttgctgc agtttgagag ttggcattct tttgatacaa atccactata tgctcttctc
     2641 cacgaggcca tatactgtga ggtatagtct cttcaaattt ctcatctttt tttctatttc
     2701 agacaattac ttcttcttgt tatcgtttat gtttaaatgg aaactttttc agggtgcttc
     2761 atcaggatgg tctgctcata gattacggga taaatatgag tacaaattcg acgcaatgaa
     2821 ggcagtcaaa gaaagtcagc ctgtactctt cacaggagag gtacaggttt tgagtatttc
     2881 taattgtatg gctcatagct tggatgattg gtccacattg cttaactaaa atagaatttg
     2941 gatttgtata gatgatattt ccatggatgt ttgatgaaat tcatgccttg aagccattta
     3001 aagctgccgc cgatcttttg gccaagaaag aggattggcc tccattatat gatgttccta
     3061 gattgcagaa taacaaggtc tgtttcagaa tcttgatcgt tctttcatat ggatcaaata
     3121 attttactct gtttcacatg atttcttttt gtaggtgcct gttgctgcag cggtttacta
     3181 cgaagatatg tatgtaaact tcaagctggt gacggaaaca gcttctcata tatcgggtat
     3241 cagactttgg gtaacgaatg agtttatgca ttcgggtctt cgtgatgctg gaagacaaat
     3301 tattgatcat ttgttgggaa tgatcaatgg gtgaccgaat tcctatctag acccagcttt
     3361 cttgtacaaa gtggtgataa cctagagctc gaatttcccc gatcgttcaa acatttggca
     3421 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     3481 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     3541 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     3601 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     3661 ggttccggaa ccaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
     3721 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3781 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctattaa ttcccgatct
     3841 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     3901 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     3961 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     4021 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     4081 gaacgatcgg ggaaattcgg gggatcgatc ccgcgaactg tggacgagaa ctgtgccacc
     4141 aagcgtaagg ccgttctctc gcatttgcct tgctaggctc gcgcgagttg ctggctgagg
     4201 cgttctcgaa atcagctctt gttcggtcgg catctactct attcctttgc cctcggacga
     4261 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg
     4321 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt
     4381 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga
     4441 tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc
     4501 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc
     4561 cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca
     4621 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc
     4681 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc
     4741 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
     4801 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
     4861 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg
     4921 accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
     4981 gcacggcggg agatgcaata ggtcaggctc tcgctgaatg ccccaatgtc aagcacttcc
     5041 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
     5101 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
     5161 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc
     5221 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatctta ttgccccccg
     5281 ggatcagatc tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     5341 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     5401 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     5461 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     5521 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatctt
     5581 gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc
     5641 ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa
     5701 accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg
     5761 cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt
     5821 ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc
     5881 ttgcggcagc gtgaagctga tccgtcgaga ttaatagaaa taaattcagc ctaattcggc
     5941 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     6001 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     6061 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     6121 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     6181 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     6241 cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg
     6301 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     6361 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     6421 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     6481 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     6541 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     6601 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     6661 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     6721 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     6781 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     6841 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     6901 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     6961 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     7021 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     7081 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     7141 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     7201 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     7261 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     7321 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
     7381 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     7441 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     7501 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8341 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     8401 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     8461 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     8521 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     8581 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     8641 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     8701 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     8761 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     8821 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     8881 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     8941 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     9001 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     9061 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     9121 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     9181 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     9241 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     9301 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     9361 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     9421 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     9481 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     9541 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     9601 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     9661 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     9721 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     9781 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
     9841 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
     9901 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
     9961 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
    10021 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
    10081 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
    10141 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
    10201 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
    10261 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
    10321 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
    10381 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
    10441 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
    10501 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
    10561 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
    10621 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
    10681 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    10741 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    10801 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    10861 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    10921 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    10981 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    11041 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    11101 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    11161 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    11221 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    11281 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    11341 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    11401 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    11461 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    11521 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    11581 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    11641 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    11701 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    11761 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    11821 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    11881 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    11941 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    12001 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    12061 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    12121 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    12181 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    12241 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    12301 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    12361 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    12421 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
