LOCUS       sequence-477961-       12660 bp    DNA     circular SYN 09-JUL-2025
DEFINITION  Stable transformation or transient expression of gene of interest in
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Kim G, Wan W, Kim N, Yi YY, Charoennit N, Lee RRQ, Liew YY, Ng SK, 
            Zhang Y, Song J, Chae E
  TITLE     Structure determinants of DANGEROUS MIX 3, an alpha/beta hydrolase, 
            for triggering NLR-mediated genetic incompatibility in plants
  JOURNAL   bioRxiv 2024.10.28.620575; doi: 
            https://doi.org/10.1101/2024.10.28.620575
REFERENCE   2  (bases 1 to 12660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.28.620575; doi: https://doi.org/10.1101/2024.10.28.620575"
COMMENT     Sequence Label: pGWB515_35S-HA-(Delta-TP)DM3(Col-0) Delta-C
FEATURES             Location/Qualifiers
     source          1..12660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1196..1285
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    1307..1331
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3502..3526)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3537..3560
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      3582..3834
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3867..3883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3867..3883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3880..3902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3891..3907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3915..3921,3922..3939,3940..3945))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3960..3981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4017..4269)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4420..5445)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5497..5676)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6182..6206
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6727..7518
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7764..8352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8253..8272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8506..8523
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8538..8678
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8604..8623)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8764..8786
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9022..9216
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9282..10355)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10784..11413)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcggg ttaattaaca tcttttaccc
     1201 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta
     1261 tccatatgac gttccagatt acgctgctca gtgcagcgct gttatcacaa gtttgtacaa
     1321 aaaagcaggc tccgaattcg cccttatgga agcgggatcg gtttacgtcg atgtagctgg
     1381 ggaatcgaag tcggagcatg ttactgggaa gtggttttcg gtgccggagc tccgactgcg
     1441 tgaccaccgg ttcatagttc ctcttgatta ttccaaaagt tctcccaaaa ttaccgtctt
     1501 tgctcgagag atcgttgcgg gtaacttgaa atttggagct ttttgattca gagctctttc
     1561 tgtgattacg aagctaaaag tttgtgtctt ttgttttttg tagttgggaa agaggagcaa
     1621 gcaatgccgt atttattgta tttgcaaggt ggtccaggat ttgaaggtcc aaggcctagt
     1681 gaagctagtg gatggattca aagagcttgt gaagagtttc gagtggtctt actcgatcaa
     1741 gtatgatgct ttcatagttt tatactcttc tctggacagt ttttttagtc tttgtgttgt
     1801 tctaattttg tttgtcctgc agcgaggaac aggcttgtcc actcctttaa catgttcctc
     1861 tatgcttcaa ttcaaatcag caaaggaatt agctgattat ttggttcact ttcgagctga
     1921 taatatagtt aaagatgctg agtttattag agttcgtctt gttcctaagg ctgatccttg
     1981 gacaattttg ggtcaggtaa tttgtctttg tattgtctat tggtcatctt gcttcagttc
     2041 ttgtcatttg attttgtatt catctttttt tcttcaaatc tacagagttt cggtggcttt
     2101 tgtgcactta catatctgag ttttgcaccc gaaggactca aacaagtgct aataactgga
     2161 ggaattccac caattggtaa agcatgtaca gcagatgatg tatatgaagc cggttttgaa
     2221 caagttgctc gccaaaatga aaaatactac aagagattcc ctcaagacat tgagattgtt
     2281 cgtgaactcg taaattactt ggctgaatct gaaggtggcg gggtgaggga ttacaacatt
     2341 tgctcaatcc actgaccagt ttagtttagt tccttacttg tctctgtttc tttgtcttta
     2401 ggtacctctc ccatcaggag gcattcttac tcctaagggt cttcaaactc ttggtctttc
     2461 tggtttagga tcaagtactg gttttgagcg cctgcattat atgtaagagt ttagcttaat
     2521 ttttatcaac cacctcacat gttctgtgtt aaatctggcc ttcttaattg atcataggtt
     2581 ggagagagta tgggatccta ttttagttac tggagctcca aagtgtatta gtcagttctt
     2641 cttaaacgct gtaagagacc cacaaaaatt tgttgttttc tgttctcagc tcaaataagg
     2701 aattcaatag tctgagacat tttatcgttg ctgcagtttg agagttggca ttcttttgat
     2761 acaaatccac tatatgctct tctccacgag gccatatact gtgaggtata gtctcttcaa
     2821 atttctcatc tttttttcta tttcagacaa ttacttcttc ttgttatcgt ttatgtttaa
     2881 atggaaactt tttcagggtg cttcatcagg atggtctgct catagattac gggataaata
     2941 tgagtacaaa ttcgacgcaa tgaaggcagt caaagaaagt cagcctgtac tcttcacagg
     3001 agaggtacag gttttgagta tttctaattg tatggctcat agcttggatg attggtccac
     3061 attgcttaac taaaatagaa tttggatttg tatagatgat atttccatgg atgtttgatg
     3121 aaattcatgc cttgaagcca tttaaagctg ccgccgatct tttggccaag aaagaggatt
     3181 ggcctccatt atatgatgtt cctagattgc agaataacaa ggtctgtttc agaatcttga
     3241 tcgttctttc atatggatca aataatttta ctctgtttca catgatttct ttttgtaggt
     3301 gcctgttgct gcagcggttt actacgaaga tatgtatgta aacttcaagc tggtgacgga
     3361 aacagcttct catatatcgg gtatcagact ttgggtaacg aatgagttta tgcattcggg
     3421 tcttcgtgat gctggaagac aaattattga tcatttgttg ggaatgatca atgggtgagg
     3481 atccatggaa gggcgaattc gacccagctt tcttgtacaa agtggttgat aacagcgact
     3541 acaaggatga cgatgacaag gcttagagct cgaatttccc cgatcgttca aacatttggc
     3601 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     3661 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     3721 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     3781 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
     3841 tggttccgga accaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     3901 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     3961 tgagtgagct aactcacatt aggctgaatt aggcgcgcct atttctatta attcccgatc
     4021 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
     4081 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
     4141 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
     4201 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
     4261 tgaacgatcg gggaaattcg ggggatcgat cccgcgaact gtggacgaga actgtgccac
     4321 caagcgtaag gccgttctct cgcatttgcc ttgctaggct cgcgcgagtt gctggctgag
     4381 gcgttctcga aatcagctct tgttcggtcg gcatctactc tattcctttg ccctcggacg
     4441 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
     4501 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
     4561 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
     4621 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
     4681 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
     4741 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
     4801 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
     4861 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     4921 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     4981 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     5041 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
     5101 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     5161 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat gccccaatgt caagcacttc
     5221 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     5281 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     5341 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     5401 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctt attgcccccc
     5461 gggatcagat ctggattgag agtgaatatg agactctaat tggataccga ggggaattta
     5521 tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc
     5581 gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa
     5641 acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc
     5701 ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatct
     5761 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     5821 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     5881 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     5941 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     6001 tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg
     6061 cttgcggcag cgtgaagctg atccgtcgag attaatagaa ataaattcag cctaattcgg
     6121 cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
     6181 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
     6241 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
     6301 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
     6361 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
     6421 acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg
     6481 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     6541 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     6601 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     6661 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     6721 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     6781 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     6841 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     6901 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     6961 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     7021 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     7081 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     7141 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     7201 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     7261 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     7321 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     7381 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     7441 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     7501 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
     7561 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     7621 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     7681 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7741 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7801 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7861 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7921 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7981 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8041 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8101 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8161 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8221 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8281 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8341 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8401 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8461 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8521 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     8581 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8641 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     8701 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     8761 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
     8821 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
     8881 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
     8941 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
     9001 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
     9061 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
     9121 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
     9181 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
     9241 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
     9301 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
     9361 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
     9421 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
     9481 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
     9541 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
     9601 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
     9661 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
     9721 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
     9781 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
     9841 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
     9901 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
     9961 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
    10021 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
    10081 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
    10141 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
    10201 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
    10261 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
    10321 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
    10381 cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc
    10441 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
    10501 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
    10561 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
    10621 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
    10681 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
    10741 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
    10801 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
    10861 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
    10921 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
    10981 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
    11041 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
    11101 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
    11161 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
    11221 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    11281 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    11341 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    11401 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    11461 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    11521 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    11581 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    11641 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    11701 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    11761 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    11821 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    11881 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    11941 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    12001 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    12061 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    12121 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    12181 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    12241 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    12301 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    12361 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    12421 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
    12481 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
    12541 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
    12601 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
//
