LOCUS       sequence-478230-       12635 bp    DNA     circular SYN 11-JUL-2025
DEFINITION  Stable transformation or transient expression of gene of interest in
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Kim G, Wan W, Kim N, Yi YY, Charoennit N, Lee RRQ, Liew YY, Ng SK, 
            Zhang Y, Song J, Chae E
  TITLE     Structure determinants of DANGEROUS MIX 3, an alpha/beta hydrolase, 
            for triggering NLR-mediated genetic incompatibility in plants
  JOURNAL   bioRxiv 2024.10.28.620575; doi: 
            https://doi.org/10.1101/2024.10.28.620575
REFERENCE   2  (bases 1 to 12635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.28.620575; doi: https://doi.org/10.1101/2024.10.28.620575"
COMMENT     Sequence Label: pGWB515_35S-HA-(Delta-TP)DM3(Col-0) D332R Q345R
FEATURES             Location/Qualifiers
     source          1..12635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1196..1285
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    1307..1331
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3507..3531)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3557..3809
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3842..3858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3842..3858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3855..3877)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3866..3882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3890..3896,3897..3914,3915..3920))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3935..3956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3992..4244)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4395..5420)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5472..5651)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6157..6181
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6702..7493
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7739..8327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8228..8247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8481..8498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8513..8653
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8579..8598)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8739..8761
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8997..9191
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9257..10330)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10759..11388)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcggg ttaattaaca tcttttaccc
     1201 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta
     1261 tccatatgac gttccagatt acgctgctca gtgcagcgct gttatcacaa gtttgtacaa
     1321 aaaagcaggc tttcgaattc caatggaagc gggatcggtt tacgtcgatg tagctgggga
     1381 atcgaagtcg gagcatgtta ctgggaagtg gttttcggtg ccggagctcc gactgcgtga
     1441 ccaccggttc atagttcctc ttgattattc caaaagttct cccaaaatta ccgtctttgc
     1501 tcgagagatc gttgcgggta acttgaaatt tggagctttt tgattcagag ctctttctgt
     1561 gattacgaag ctaaaagttt gtgtcttttg ttttttgtag ttgggaaaga ggagcaagca
     1621 atgccgtatt tattgtattt gcaaggtggt ccaggatttg aaggtccaag gcctagtgaa
     1681 gctagtggat ggattcaaag agcttgtgaa gagtttcgag tggtcttact cgatcaagta
     1741 tgatgctttc atagttttat actcttctct ggacagtttt tttagtcttt gtgttgttct
     1801 aattttgttt gtcctgcagc gaggaacagg cttgtccact cctttaacat gttcctctat
     1861 gcttcaattc aaatcagcaa aggaattagc tgattatttg gttcactttc gagctgataa
     1921 tatagttaaa gatgctgagt ttattagagt tcgtcttgtt cctaaggctg atccttggac
     1981 aattttgggt caggtaattt gtctttgtat tgtctattgg tcatcttgct tcagttcttg
     2041 tcatttgatt ttgtattcat ctttttttct tcaaatctac agagtttcgg tggcttttgt
     2101 gcacttacat atctgagttt tgcacccgaa ggactcaaac aagtgctaat aactggagga
     2161 attccaccaa ttggtaaagc atgtacagca gatgatgtat atgaagccgg ttttgaacaa
     2221 gttgctcgcc aaaatgaaaa atactacaag agattccctc aagacattga gattgttcgt
     2281 gaactcgtaa attacttggc tgaatctgaa ggtggcgggg tgagggatta caacatttgc
     2341 tcaatccact gaccagttta gtttagttcc ttacttgtct ctgtttcttt gtctttaggt
     2401 acctctccca tcaggaggca ttcttactcc taagggtctt caaactcttg gtctttctgg
     2461 tttaggatca agtactggtt ttgagcgcct gcattatatg taagagttta gcttaatttt
     2521 tatcaaccac ctcacatgtt ctgtgttaaa tctggccttc ttaattgatc ataggttgga
     2581 gagagtatgg gatcctattt tagttactgg agctccaaag tgtattagtc ggttcttctt
     2641 aaacgctgta agagacccac aaaaatttgt tgttttctgt tctcagctca aataaggaat
     2701 tcaatagtct gagacatttt atcgttgctg cagtttgaga gttggcattc ttttgataca
     2761 aatccactat atgctcttct ccacgaggcc atatactgtg aggtatagtc tcttcaaatt
     2821 tctcatcttt ttttctattt cagacaatta cttcttcttg ttatcgttta tgtttaaatg
     2881 gaaacttttt cagggtgctt catcaggatg gtctgctcat agattacggg ataaatatga
     2941 gtacaaattc gacgcaatga aggcagtcaa agaaagtcag cctgtactct tcacaggaga
     3001 ggtacaggtt ttgagtattt ctaattgtat ggctcatagc ttggatgatt ggtccacatt
     3061 gcttaactaa aatagaattt ggatttgtat agatgatatt tccatggatg tttgatgaaa
     3121 ttcatgcctt gaagccattt aaagctgccg ccgatctttt ggccaagaaa gaggattggc
     3181 ctccattata tgatgttcct agattgcaga ataacaaggt ctgtttcaga atcttgatcg
     3241 ttctttcata tggatcaaat aattttactc tgtttcacat gatttctttt tgtaggtgcc
     3301 tgttgctgca gcggtttact acgaagatat gtatgtaaac ttcaagctgg tgacggaaac
     3361 agcttctcat atatcgggta tcagactttg ggtaacgaat gagtttatgc attcgggtct
     3421 tcgtgatgct ggaagacaaa ttattgatca tttgttggga atgatcaatg ggaaaaagcc
     3481 tctcttttga ccgaattcct atctagaccc agctttcttg tacaaagtgg tgataaccta
     3541 gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     3601 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     3661 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     3721 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     3781 gcgcgcggtg tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca
     3841 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3901 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaggct
     3961 gaattaggcg cgcctatttc tattaattcc cgatctagta acatagatga caccgcgcgc
     4021 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat
     4081 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt
     4141 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca
     4201 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa attcggggga
     4261 tcgatcccgc gaactgtgga cgagaactgt gccaccaagc gtaaggccgt tctctcgcat
     4321 ttgccttgct aggctcgcgc gagttgctgg ctgaggcgtt ctcgaaatca gctcttgttc
     4381 ggtcggcatc tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta
     4441 tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt
     4501 gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa
     4561 gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg
     4621 agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag
     4681 cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg
     4741 tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg
     4801 tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag
     4861 tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg
     4921 tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc
     4981 catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta
     5041 agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt
     5101 tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc
     5161 aggctctcgc tgaatgcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca
     5221 aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg
     5281 acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc
     5341 tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg
     5401 gtgagttcag gctttttcat atcttattgc cccccgggat cagatctgga ttgagagtga
     5461 atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga
     5521 caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt
     5581 tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg gctccttcaa
     5641 cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc
     5701 ataacgtgac tcccttaatt ctccgctcat gatcttgatc ccctgcgcca tcagatcctt
     5761 ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc
     5821 ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat
     5881 gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata
     5941 gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgtg
     6001 ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga agctgatccg
     6061 tcgagattaa tagaaataaa ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt
     6121 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
     6181 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     6241 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     6301 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     6361 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa
     6421 ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     6481 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     6541 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     6601 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     6661 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg
     6721 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     6781 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     6841 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     6901 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     6961 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     7021 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     7081 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     7141 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     7201 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     7261 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     7321 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     7381 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     7441 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag
     7501 ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc
     7561 actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta
     7621 agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc aaaatccctt
     7681 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7741 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7801 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7861 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     7921 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7981 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8041 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8101 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8161 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8221 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8281 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8341 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8401 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8461 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     8521 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     8581 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     8641 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     8701 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     8761 gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt
     8821 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
     8881 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
     8941 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
     9001 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
     9061 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
     9121 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
     9181 tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta
     9241 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
     9301 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
     9361 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
     9421 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
     9481 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
     9541 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
     9601 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
     9661 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
     9721 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
     9781 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
     9841 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
     9901 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
     9961 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
    10021 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
    10081 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg
    10141 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
    10201 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
    10261 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
    10321 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac
    10381 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
    10441 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
    10501 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
    10561 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
    10621 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
    10681 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
    10741 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
    10801 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
    10861 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
    10921 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
    10981 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
    11041 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
    11101 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
    11161 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
    11221 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
    11281 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
    11341 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
    11401 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
    11461 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
    11521 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
    11581 gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc
    11641 tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct
    11701 cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga
    11761 aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt
    11821 acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct
    11881 ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa
    11941 acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg
    12001 aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg
    12061 tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct
    12121 cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca
    12181 caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg
    12241 tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt
    12301 ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg
    12361 cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac
    12421 taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt
    12481 aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg
    12541 cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt
    12601 ggcatgcaca tacaaatgga cgaacggata aacct
//
