LOCUS       sequence-478238-       10616 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  Expresses eA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Xue N, Liu X, Zhang D, Wu Y, Zhong Y, Wang J, Fan W, Jiang H, Zhu B,
            Ge X, Gonzalez RVL, Chen L, Zhang S, She P, Zhong Z, Sun J, Chen X, 
            Wang L, Gu Z, Zhu P, Liu M, Li D, Zhong TP, Zhang X
  TITLE     Improving adenine and dual base editors through introduction of 
            TadA-8e and Rad51DBD.
  JOURNAL   Nat Commun. 2023 Mar 3;14(1):1224. doi: 10.1038/s41467-023-36887-1.
  PUBMED    36869044
REFERENCE   2  (bases 1 to 10616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-03-3"; volume: "14(1):1224. doi"; pages: " 
            10.1038/s41467-023-36887-1"
COMMENT     Sequence Label: eA&C-BEmax
FEATURES             Location/Qualifiers
     source          1..10616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             445..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1648..1671,1672..1674,1675..5748)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1648..5748
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5749..5796
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5806..5859
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5860..6108
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6139..6387
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6430..6450
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6460..6516
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6517..6519,6520..6522,6523..7233)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6517..6519,6520..6522,6523..7233)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6823..6842)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7170..7191
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7246..7266
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7289..7306)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7295..7519
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7603..7625)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7614..7630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7638..7644,7645..7662,7663..7668))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7683..7704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7821..7838)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7992..8580)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8072..8091)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8751..9542,9543..9611))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9374..9393
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9612..9716)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9795..9814)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10006..10254
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     10006..10026
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     10177..10196
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10273..10348
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        join(10368..10616,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcgacag cctgctgatg
      481 aaccggagaa agttcctgta tcagtttaag aatgtgaggt gggcaaaggg caggcgcgag
      541 acctacctgt gctatgtggt gaagcggaga gatagcgcca catctttcag cctggacttt
      601 ggctacctgc ggaacaagaa tggctgccac gtggagctgc tgtttctgag atacatctcc
      661 gactgggatc tggacccagg caggtgttat cgcgtgacct ggttcacatc ctggtctccc
      721 tgctacgatt gtgcccggca cgtggccgac tttctgagag gcaaccctaa tctgtctctg
      781 aggatcttca ccgcccgcct gtatttttgc gaggatagga aggccgagcc agagggactg
      841 aggcgcctgc acagggccgg cgtgcagatc gccatcatga cattcaagga ctacttttat
      901 tgttggaaca ccttcgtgga gaatcacgag cggacattta aggcctggga gggactgcac
      961 gagaactccg tgcggctgtc tagacagctg cggagaatcc tgctgccaga ggccgccgcc
     1021 aaggaagctg ccgccaagga ggccgccgcc aagtctgagg tggagttttc ccacgagtac
     1081 tggatgagac atgccctgac cctggccaag agggcacggg atgagaggga ggtgcctgtg
     1141 ggagccgtgc tggtgctgaa caatagagtg atcggcgagg gctggaacag agccatcggc
     1201 ctgcacgacc caacagccca tgccgaaatt atggccctga gacagggcgg cctggtcatg
     1261 cagaactaca gactgattga cgccaccctg tacgtgacat tcgagccttg cgtgatgtgc
     1321 gccggcgcca tgatccactc taggatcggc cgcgtggtgt ttggcgtgag gaactcaaaa
     1381 agaggcgccg caggctccct gatgaacgtg ctgaactacc ccggcatgaa tcaccgcgtc
     1441 gaaattaccg agggaatcct ggcagatgaa tgtgccgccc tgctgtgcga tttctatcgg
     1501 atgcctagac aggtgttcaa tgctcagaag aaggcccaga gctccatcaa ctccggagga
     1561 tctagcggag gctcctctgg ctctgagaca cctggcacaa gcgagagcgc aacacctgaa
     1621 agcagcgggg gcagcagcgg ggggtcagac aagaagtaca gcatcggcct ggccatcggc
     1681 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc
     1741 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
     1801 ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac
     1861 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag
     1921 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag
     1981 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
     2041 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg
     2101 ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc
     2161 gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac
     2221 aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg
     2281 tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag
     2341 aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac ccccaacttc
     2401 aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac
     2461 gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc
     2521 gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc
     2581 accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg
     2641 accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc
     2701 gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc
     2761 tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag
     2821 ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac
     2881 cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc
     2941 ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg
     3001 ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc
     3061 atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc
     3121 gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc
     3181 ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag
     3241 ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg
     3301 ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc
     3361 gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc
     3421 acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac
     3481 gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc
     3541 gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag
     3601 cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac
     3661 aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac
     3721 ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag
     3781 gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc
     3841 attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc
     3901 cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag
     3961 ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc
     4021 agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac
     4081 ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg
     4141 ctgtccgact acgatgtgga ccatatcgtg cctcagagct ttctgaagga cgactccatc
     4201 gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc
     4261 gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt
     4321 acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat
     4381 aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca
     4441 cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa
     4501 gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt
     4561 tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc
     4621 gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac
     4681 tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct
     4741 accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg
     4801 gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc
     4861 gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg
     4921 aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc
     4981 aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc
     5041 ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc
     5101 aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc
     5161 agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag
     5221 gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga
     5281 atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat
     5341 gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat
     5401 gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag
     5461 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc
     5521 gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg
     5581 tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac
     5641 cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc
     5701 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacaa aaggccggcg
     5761 gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat ccggagaggg cagaggaagt
     5821 ctgctaacat gcggtgacgt cgaggagaat cctggcccaa ctaatctgag cgacatcatt
     5881 gagaaggaga ctgggaaaca gctggtcatt caggagtcca tcctgatgct gcctgaggag
     5941 gtggaggaag tgatcggcaa caagccagag tctgacatcc tggtgcacac cgcctacgac
     6001 gagtccacag atgagaatgt gatgctgctg acctctgacg cccccgagta taagccttgg
     6061 gccctggtca tccaggattc taacggcgag aataagatca agatgctgag cggaggatcc
     6121 ggaggatctg gaggcagcac caacctgtct gacatcatcg agaaggagac aggcaagcag
     6181 ctggtcatcc aggagagcat cctgatgctg cccgaagaag tcgaagaagt gatcggaaac
     6241 aagcctgaga gcgatatcct ggtccatacc gcctacgacg agagtaccga cgaaaatgtg
     6301 atgctgctga catccgacgc cccagagtat aagccctggg ctctggtcat ccaggattcc
     6361 aacggagaga acaaaatcaa aatgctgtct ggcggctcaa aaagaaccgc cgacggcagc
     6421 gaattcgagc ccaagaagaa gaggaaagtc ggaagcggag ctactaactt cagcctgctg
     6481 aagcaggctg gagacgtgga ggagaaccct ggacctatgg tgagcaaggg cgaggagctg
     6541 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     6601 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     6661 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctatgga
     6721 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     6781 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     6841 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     6901 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     6961 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     7021 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     7081 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     7141 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     7201 gggatcactc tcggcatgga cgagctgtac aagtctggtg gttctcccaa gaagaaaagg
     7261 aaagtctaag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     7321 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     7381 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     7441 ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg
     7501 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg
     7561 acctctagct agagcttggc gtaatcatgg tcatagatgt ttcctgtgtg aaattgttat
     7621 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctagggtgcc
     7681 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7741 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7801 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7861 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7921 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7981 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8041 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8101 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8161 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8221 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8281 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8341 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8401 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8461 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8521 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8581 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8641 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8701 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8761 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8821 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8881 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8941 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9001 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9061 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9121 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9181 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9241 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9301 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9361 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9421 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9481 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9541 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9601 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9661 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9721 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     9781 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     9841 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     9901 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     9961 gatgtacggg ccagatatac gcgtggatcc ctgcagttaa ttaaagaggg cctatttccc
    10021 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
    10081 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
    10141 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
    10201 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggtct
    10261 tcgagaagac ctgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
    10321 acttgaaaaa gtggcaccga gtcggtgctt tttttatgca tggtaccgac attgattatt
    10381 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
    10441 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
    10501 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
    10561 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatc
//
