LOCUS       sequence-478243-        9166 bp    DNA     circular SYN 11-JUL-2025
DEFINITION  PiggyBac plasmid for the insertion of a sperm-expressed red 
            fluorescent protein in Aedes aegypti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9166)
  TITLE     Mosquito sex under lock and key
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB{3xP3-EGFP; beta-2Tubulin-dsRed2}
FEATURES             Location/Qualifiers
     source          1..9166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   1172..1206
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    2622..2743
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2864..3541)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMSFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2926..2949)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     3341..3361
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     regulatory      3538..3547
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4549..4670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(4793..5506,5507..5509,5510..5512))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4796..5506,5507..5509,5510..5512))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4838..4859)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5187..5206
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5446..5467
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5509..5518
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   6775..6837
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7541..7558)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7541..7557)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7550..7572)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7766..7785)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7885..7907
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7945..7963)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8031..8135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8136..8204,8205..8996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8354..8373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga catgattacg aattcgagct cggtacccgg
     1021 ggatcctcta gagtcgacgc tcgcgcgact tggtttgcca ttctttagcg cgcgtcgcgt
     1081 cacacagctt ggccacaatg tggtttttgt caaacgaaga ttctatgacg tgtttaaagt
     1141 ttaggtcgag taaagcgcaa atctttttta accctagaaa gatagtctgc gtaaaattga
     1201 cgcatgcatt cttgaaatat tgctctctct ttctaaatag cgcgaatccg tcgctgtgca
     1261 tttaggacat ctcagtcgcc gcttggagct cccgtgaggc gtgcttgtca atgcggtaag
     1321 tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa tgacgcatga ttatctttta
     1381 cgtgactttt aagatttaac tcatacgata attatattgt tatttcatgt tctacttacg
     1441 tgataactta ttatatatat attttcttgt tatagatatc gtgactaata tataataaaa
     1501 tgggtagttc tttagacgat gagcatatcc tctctgctct tctgcaaagc gatgacgagc
     1561 ttgttggtga ggattctgac agtgaaatat cagatcacgt aagtgaagat gacgtccaga
     1621 gcgatacaga agaagcgttt atagatgagg tacatgaagt gcagccaacg tcaagcggta
     1681 gtgaaatatt agacgaacaa aatgttattg aacaaccagg ttcttcattg gcttctaaca
     1741 gaatcttgac cttgccacag aggactatta gaggtaagaa taaacattgt tggtcaactt
     1801 caaagtccac gaggcgtagc cgagtctctg cactgaacat tgtcagatct caaagaggtc
     1861 cgacgcgtat gtgccgcaat atatatgacc cacttttatg cttcaaacta ttttttactg
     1921 atgagataat ttcggaaatt gtaaaatgga caaatgctga gatatcattg aaacgtcggg
     1981 aatctatgac aggtgctaca tttcgtgaca cgaatgaaga tgaaatctat gctttctttg
     2041 gtattctggt aatgacagca gtgagaaaag ataaccacat gtccacagat gacctctttg
     2101 atcgatcttt gtcaatggtg tacgtctctg taatgagtcg tgatcgtttt gattttttga
     2161 tacgatgtct tagaatggat gacaaaagta tacggcccac acttcgagaa aacgatgtat
     2221 ttactcctgt tagaaaaata tgggatctct ttatccatca gtgcatacaa aattacactc
     2281 caggggctca tttgaccata gatgaacagt tacttggttt tagaggacgg tgtccgttta
     2341 ggatgtatat cccaaacaag ccaagtaagt atggaataaa aatcctcatg atgtgtgaca
     2401 gtggtacgaa gtatatgata aatggaatgc cttatttggg aagaggaaca cagaccaacg
     2461 gagtaccact cggtgaatac tacgtgaagg agttatcaaa gcctgtgcac ggtagttgtc
     2521 gtaatattac gtgtgacaat tggttcacct caatcccttt ggcaaaaaac ttactacaag
     2581 aaccgtataa gttcgagatc ggccggccta ggcgcgcccc ttaagataca ttgatgagtt
     2641 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2701 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2761 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     2821 ctacaaatgt ggtatggctg attatgatct agagtcgcgg ccgctacagg aacaggtggt
     2881 ggcggccctc ggtgcgctcg tactgctcca cgatggtgta gtcctcgttg tgggaggtga
     2941 tgtccagctt ggcgtccacg tagtagtagc cgggcagctg cacgggcttc ttggccatgt
     3001 agatggactt gaactccacc aggtagtggc cgccgtcctt cagcttcagg gccttgtggg
     3061 tctcgccctt cagcacgccg tcgcgggggt acaggcgctc ggtggaggcc tcccagccca
     3121 tggtcttctt ctgcatcacg gggccgtcgg aggggaagtt cacgccgatg aacttcacct
     3181 tgtagatgaa gcagccgtcc tgcagggagg agtcctgggt cacggtcgcc acgccgccgt
     3241 cctcgaagct catcacgcgc tcccacttga agccctcggg gaaggacagc ttcttgtagt
     3301 cggggatgtc ggcggggtgc ttcacgtaca ccttggagcc gtactggaac tggggggaca
     3361 ggatgtccca ggcgaagggc agggggccgc ccttggtcac cttcagcttc acggtgttgt
     3421 ggccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg
     3481 tgccctccat gcgcaccttg aagcgcatga actcggtgat gacgttctcg gaggaggcca
     3541 tggtggcgac cggtggatcc cctggagcac ttctagcggt tccaaaattg ggcttcaaaa
     3601 tatgcttctt ccgatgcttg aagatggaag cagaatggtt ccaaatcacg tccgcgttcc
     3661 tcggtggtga aaatcgaagt gaaaattttt ccaactgtcg tcctccgggt cgtcaagaag
     3721 aaggatggcc ttccatgaca cactgtgatt gatttacatt gtggcggcag tatgggcccc
     3781 caaaacggga gcgacgcgtt caacatgtgc tactggatgc attcgcttcg aagtctgttg
     3841 tcacgttggg ggctgtccat atgccacgtg gttgttatgg ggtggagaag ggtactgctc
     3901 aagcatcacg gtgcatacaa aatttgaaaa tttttgtgtg gacgaaagat cataaggagg
     3961 agcgtgaaaa ttcataaaaa atgaccacgt ggtttatgga cgaaacaaaa gcgatatatg
     4021 agccacaaga acatagcgtg attcaccccc acattagggg aaacatgcaa gtcctttgca
     4081 taaactatca aaatttggcg gaaaataata gtgttgaaat gaataataac agttacaact
     4141 aggtattagt tcttcctctt ttcggcatta cgtctccttt gtggcagagc atgtttctga
     4201 gtttaaaaac catcatttat tttttgttga ttgctgatga ttgaagattg aatgatagat
     4261 ttttgaacct taaaacaaaa aagttaacgc gatttaaact attatgaggg gtttaaaaat
     4321 tctaacgttc agagtgtcta ctaatttaca gaaatgaaat tccctgatat ttctaggttt
     4381 tccaggcttg gaaaaaaatc caggtttaag aaagtttaca acatacacaa attattgatg
     4441 aatttttcat tttagataag tagtaatcat tttcaaagct aggagttttg cttcgaaata
     4501 cttaaacaaa aaatctaagg ggcgcgccgt acgcgtatcg ataagcttta agatacattg
     4561 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4621 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4681 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     4741 aaaacctcta caaatgtggt atggctgatt atgatctaga gtcgcggccg ctttacttgt
     4801 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
     4861 gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt
     4921 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
     4981 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
     5041 gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
     5101 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
     5161 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
     5221 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
     5281 ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc
     5341 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
     5401 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
     5461 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcga
     5521 ccggtggatc ccgggcccgc ggtaccgtcg actctagcgg taccccgatt gtttagcttg
     5581 ttcagctgcg cttgtttatt tgcttagctt tcgcttagcg acgtgttcac tttgcttgtt
     5641 tgaattgaat tgtcgctccg tagacgaagc gcctctattt atactccggc ggtcgagggt
     5701 tcgaaatcga taagcttgga tcctaattga attagctcta attgaattag tctctaattg
     5761 aattagatcc ccgggcgagc tcgaattaac cattgtggga accgtgcgat caaacaaacg
     5821 cgagataccg gaagtactga aaaacagtcg ctccaggcca gtgggaacat cgatgttttg
     5881 ttttgacgga ccccttactc tcgtctcata taaaccgaag ccagctaaga tggtatactt
     5941 attatcatct tgtgatgagg atgcttctat caacgaaagt accggtaaac cgcaaatggt
     6001 tatgtattat aatcaaacta aaggcggagt ggacacgcta gaccaaatgt gttctgtgat
     6061 gacctgcagt aggaagacga ataggtggcc tatggcatta ttgtacggaa tgataaacat
     6121 tgcctgcata aattctttta ttatatacag ccataatgtc agtagcaagg gagaaaaggt
     6181 tcaaagtcgc aaaaaattta tgagaaacct ttacatgagc ctgacgtcat cgtttatgcg
     6241 taagcgttta gaagctccta ctttgaagag atatttgcgc gataatatct ctaatatttt
     6301 gccaaatgaa gtgcctggta catcagatga cagtactgaa gagccagtaa tgaaaaaacg
     6361 tacttactgt acttactgcc cctctaaaat aaggcgaaag gcaaatgcat cgtgcaaaaa
     6421 atgcaaaaaa gttatttgtc gagagcataa tattgatatg tgccaaagtt gtttctgact
     6481 gactaataag tataatttgt ttctattatg tataagttaa gctaattact tattttataa
     6541 tacaacatga ctgtttttaa agtacaaaat aagtttattt ttgtaaaaga gagaatgttt
     6601 aaaagttttg ttactttata gaagaaattt tgagtttttg ttttttttta ataaataaat
     6661 aaacataaat aaattgtttg ttgaatttat tattagtatg taagtgtaaa tataataaaa
     6721 cttaatatct attcaaatta ataaataaac ctcgatatac agaccgataa aacacatgcg
     6781 tcaattttac gcatgattat ctttaacgta cgtcacaata tgattatctt tctagggtta
     6841 aataatagtt tctaattttt ttattattca gcctgctgtc gtgaataccg tatatctcaa
     6901 cgctgtctgt gagattgtcg tattctagcc tttttagttt ttcgctcatc gacttgatat
     6961 tgtccgacac attttcgtcg atttgcgttt tgatcaaaga cttgagcaga gacacgttaa
     7021 tcaactgttc aaattgatcc atattaacga tatcaacccg atgcgtatat ggtgcgtaaa
     7081 atatattttt taaccctctt atactttgca ctctgcgtta atacgcgttc gtgtacagac
     7141 gtaatcatgt tttctttttt ggataaaact cctactgagt ttgacctcat attagaccct
     7201 cacaagttgc aaaacgtggc attttttacc aatgaagaat ttaaagttat tttaaaaaat
     7261 ttcatcacag atttaaagaa gaaccaaaaa ttaaattatt tcaacagttt aatcgaccag
     7321 ttaatcaacg tgtacacaga cgcgtcggca aaaaacacgc agcccgacgt gttggctaaa
     7381 attattaaat caacttgtgt tatagtcacg gatttgccgt ccaacgtgtt cctcaaaaag
     7441 ttgaagacca acaagtttac ggacactatt aattatttga ttttgcccca cttcattttg
     7501 tgggatcaca attttgttat atttttaaac aaagcttggc actggccgtc gttttacaac
     7561 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7621 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7681 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7741 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     7801 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     7861 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     7921 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     7981 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8041 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8101 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8161 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8221 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8281 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8341 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8401 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8461 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8521 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8581 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8641 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8701 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8761 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8821 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8881 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8941 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9001 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9061 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9121 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
