LOCUS       sequence-478278-       12632 bp    DNA     circular SYN 11-JUL-2025
DEFINITION  Stable transformation or transient expression of gene of interest in
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12632)
  AUTHORS   Kim G, Wan W, Kim N, Yi YY, Charoennit N, Lee RRQ, Liew YY, Ng SK, 
            Zhang Y, Song J, Chae E
  TITLE     Structure determinants of DANGEROUS MIX 3, an alpha/beta hydrolase, 
            for triggering NLR-mediated genetic incompatibility in plants
  JOURNAL   bioRxiv 2024.10.28.620575; doi: 
            https://doi.org/10.1101/2024.10.28.620575
REFERENCE   2  (bases 1 to 12632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.28.620575; doi: https://doi.org/10.1101/2024.10.28.620575"
COMMENT     Sequence Label: pGWB515_35S-HA-(Delta-TP)DM3(Col-0) D332A Q345A 
            N349A
FEATURES             Location/Qualifiers
     source          1..12632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1196..1285
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    1307..1331
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3504..3528)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3554..3806
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3839..3855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3839..3855)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3852..3874)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3863..3879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3887..3893,3894..3911,3912..3917))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3932..3953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3989..4241)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4392..5417)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5469..5648)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6154..6178
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6699..7490
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7736..8324
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8225..8244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8478..8495
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8510..8650
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8576..8595)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8736..8758
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8994..9188
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9254..10327)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10756..11385)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcggg ttaattaaca tcttttaccc
     1201 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta
     1261 tccatatgac gttccagatt acgctgctca gtgcagcgct gttatcacaa gtttgtacaa
     1321 aaaagcaggc tccgaattcg cccttatgga agcgggatcg gtttacgtcg atgtagctgg
     1381 ggaatcgaag tcggagcatg ttactgggaa gtggttttcg gtgccggagc tccgactgcg
     1441 tgaccaccgg ttcatagttc ctcttgatta ttccaaaagt tctcccaaaa ttaccgtctt
     1501 tgctcgagag atcgttgcgg gtaacttgaa atttggagct ttttgattca gagctctttc
     1561 tgtgattacg aagctaaaag tttgtgtctt ttgttttttg tagttgggaa agaggagcaa
     1621 gcaatgccgt atttattgta tttgcaaggt ggtccaggat ttgaaggtcc aaggcctagt
     1681 gaagctagtg gatggattca aagagcttgt gaagagtttc gagtggtctt actcgatcaa
     1741 gtatgatgct ttcatagttt tatactcttc tctggacagt ttttttagtc tttgtgttgt
     1801 tctaattttg tttgtcctgc agcgaggaac aggcttgtcc actcctttaa catgttcctc
     1861 tatgcttcaa ttcaaatcag caaaggaatt agctgattat ttggttcact ttcgagctga
     1921 taatatagtt aaagatgctg agtttattag agttcgtctt gttcctaagg ctgatccttg
     1981 gacaattttg ggtcaggtaa tttgtctttg tattgtctat tggtcatctt gcttcagttc
     2041 ttgtcatttg attttgtatt catctttttt tcttcaaatc tacagagttt cggtggcttt
     2101 tgtgcactta catatctgag ttttgcaccc gaaggactca aacaagtgct aataactgga
     2161 ggaattccac caattggtaa agcatgtaca gcagatgatg tatatgaagc cggttttgaa
     2221 caagttgctc gccaaaatga aaaatactac aagagattcc ctcaagacat tgagattgtt
     2281 cgtgaactcg taaattactt ggctgaatct gaaggtggcg gggtgaggga ttacaacatt
     2341 tgctcaatcc actgaccagt ttagtttagt tccttacttg tctctgtttc tttgtcttta
     2401 ggtacctctc ccatcaggag gcattcttac tcctaagggt cttcaaactc ttggtctttc
     2461 tggtttagga tcaagtactg gttttgagcg cctgcattat atgtaagagt ttagcttaat
     2521 ttttatcaac cacctcacat gttctgtgtt aaatctggcc ttcttaattg atcataggtt
     2581 ggagagagta tgggctccta ttttagttac tggagctcca aagtgtatta gtgcgttctt
     2641 cttagccgct gtaagagacc cacaaaaatt tgttgttttc tgttctcagc tcaaataagg
     2701 aattcaatag tctgagacat tttatcgttg ctgcagtttg agagttggca ttcttttgat
     2761 acaaatccac tatatgctct tctccacgag gccatatact gtgaggtata gtctcttcaa
     2821 atttctcatc tttttttcta tttcagacaa ttacttcttc ttgttatcgt ttatgtttaa
     2881 atggaaactt tttcagggtg cttcatcagg atggtctgct catagattac gggataaata
     2941 tgagtacaaa ttcgacgcaa tgaaggcagt caaagaaagt cagcctgtac tcttcacagg
     3001 agaggtacag gttttgagta tttctaattg tatggctcat agcttggatg attggtccac
     3061 attgcttaac taaaatagaa tttggatttg tatagatgat atttccatgg atgtttgatg
     3121 aaattcatgc cttgaagcca tttaaagctg ccgccgatct tttggccaag aaagaggatt
     3181 ggcctccatt atatgatgtt cctagattgc agaataacaa ggtctgtttc agaatcttga
     3241 tcgttctttc atatggatca aataatttta ctctgtttca catgatttct ttttgtaggt
     3301 gcctgttgct gcagcggttt actacgaaga tatgtatgta aacttcaagc tggtgacgga
     3361 aacagcttct catatatcgg gtatcagact ttgggtaacg aatgagttta tgcattcggg
     3421 tcttcgtgat gctggaagac aaattattga tcatttgttg ggaatgatca atgggaaaaa
     3481 gcctctcttt tagggcgaat tcgacccagc tttcttgtac aaagtggtga taacctagag
     3541 ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     3601 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     3661 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     3721 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     3781 cgcggtgtca tctatgttac tagatcggga attggttccg gaaccaattc gtaatcatgt
     3841 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     3901 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaggctgaa
     3961 ttaggcgcgc ctatttctat taattcccga tctagtaaca tagatgacac cgcgcgcgat
     4021 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     4081 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     4141 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     4201 ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggaaatt cgggggatcg
     4261 atcccgcgaa ctgtggacga gaactgtgcc accaagcgta aggccgttct ctcgcatttg
     4321 ccttgctagg ctcgcgcgag ttgctggctg aggcgttctc gaaatcagct cttgttcggt
     4381 cggcatctac tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
     4441 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
     4501 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
     4561 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
     4621 atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
     4681 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
     4741 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
     4801 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
     4861 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
     4921 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
     4981 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
     5041 tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg
     5101 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
     5161 ctctcgctga atgccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
     5221 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
     5281 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
     5341 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
     5401 agttcaggct ttttcatatc ttattgcccc ccgggatcag atctggattg agagtgaata
     5461 tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa
     5521 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct
     5581 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt
     5641 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata
     5701 acgtgactcc cttaattctc cgctcatgat cttgatcccc tgcgccatca gatccttggc
     5761 ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca
     5821 gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta
     5881 agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc
     5941 cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtgttc
     6001 cgcttccttt agcagccctt gcgccctgag tgcttgcggc agcgtgaagc tgatccgtcg
     6061 agattaatag aaataaattc agcctaattc ggcgttaatt cagtacatta aaaacgtccg
     6121 caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc
     6181 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag
     6241 cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat
     6301 gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc
     6361 tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaattc
     6421 gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     6481 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     6541 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     6601 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     6661 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg gtgatcgccg
     6721 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     6781 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     6841 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     6901 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     6961 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     7021 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     7081 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     7141 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     7201 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     7261 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     7321 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     7381 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     7441 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctagcta
     7501 gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact
     7561 aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc
     7621 gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa atcccttaac
     7681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7861 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     7921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8401 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8461 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     8521 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     8581 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     8641 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     8701 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     8761 ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga
     8821 gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt
     8881 ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg
     8941 accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac
     9001 aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc
     9061 cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg
     9121 ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc
     9181 tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca
     9241 tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca
     9301 tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat
     9361 ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac
     9421 tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg
     9481 ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg
     9541 tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga
     9601 tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg
     9661 cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg
     9721 caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc
     9781 catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg
     9841 gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca
     9901 tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta
     9961 cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca
    10021 gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca
    10081 tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac
    10141 cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt
    10201 caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact
    10261 tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac
    10321 cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac
    10381 ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc
    10441 ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact
    10501 catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg
    10561 taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca
    10621 actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct
    10681 tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct
    10741 ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc
    10801 gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc
    10861 tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag
    10921 cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc
    10981 cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc
    11041 ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt
    11101 gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg
    11161 ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag
    11221 cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac
    11281 taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt
    11341 cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt
    11401 atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac
    11461 tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc
    11521 ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt
    11581 gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct
    11641 tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt
    11701 ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg
    11761 caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca
    11821 gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt
    11881 cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact
    11941 tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac
    12001 gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg
    12061 tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg
    12121 caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa
    12181 cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc
    12241 cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt
    12301 tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc
    12361 tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa
    12421 aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg
    12481 acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
    12541 tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc
    12601 atgcacatac aaatggacga acggataaac ct
//
