LOCUS       sequence-478286-       12633 bp    DNA     circular SYN 11-JUL-2025
DEFINITION  Stable transformation or transient expression of gene of interest in
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12633)
  AUTHORS   Kim G, Wan W, Kim N, Yi YY, Charoennit N, Lee RRQ, Liew YY, Ng SK, 
            Zhang Y, Song J, Chae E
  TITLE     Structure determinants of DANGEROUS MIX 3, an alpha/beta hydrolase, 
            for triggering NLR-mediated genetic incompatibility in plants
  JOURNAL   bioRxiv 2024.10.28.620575; doi: 
            https://doi.org/10.1101/2024.10.28.620575
REFERENCE   2  (bases 1 to 12633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.28.620575; doi: https://doi.org/10.1101/2024.10.28.620575"
COMMENT     Sequence Label: pGWB515_35S-HA-(Delta-TP)DM3(Col-0) Y460E V484E
FEATURES             Location/Qualifiers
     source          1..12633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1196..1285
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    1307..1331
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3504..3528)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3555..3807
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3840..3856)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3840..3856)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3853..3875)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3864..3880
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3888..3894,3895..3912,3913..3918))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3933..3954
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3990..4242)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4393..5418)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5470..5649)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6155..6179
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6700..7491
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7737..8325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8226..8245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8479..8496
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8511..8651
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8577..8596)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8737..8759
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8995..9189
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9255..10328)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10757..11386)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcggg ttaattaaca tcttttaccc
     1201 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta
     1261 tccatatgac gttccagatt acgctgctca gtgcagcgct gttatcacaa gtttgtacaa
     1321 aaaagcaggc tccgaattcg cccttatgga agcgggatcg gtttacgtcg atgtagctgg
     1381 ggaatcgaag tcggagcatg ttactgggaa gtggttttcg gtgccggagc tccgactgcg
     1441 tgaccaccgg ttcatagttc ctcttgatta ttccaaaagt tctcccaaaa ttaccgtctt
     1501 tgctcgagag atcgttgcgg gtaacttgaa atttggagct ttttgattca gagctctttc
     1561 tgtgattacg aagctaaaag tttgtgtctt ttgttttttg tagttgggaa agaggagcaa
     1621 gcaatgccgt atttattgta tttgcaaggt ggtccaggat ttgaaggtcc aaggcctagt
     1681 gaagctagtg gatggattca aagagcttgt gaagagtttc gagtggtctt actcgatcaa
     1741 gtatgatgct ttcatagttt tatactcttc tctggacagt ttttttagtc tttgtgttgt
     1801 tctaattttg tttgtcctgc agcgaggaac aggcttgtcc actcctttaa catgttcctc
     1861 tatgcttcaa ttcaaatcag caaaggaatt agctgattat ttggttcact ttcgagctga
     1921 taatatagtt aaagatgctg agtttattag agttcgtctt gttcctaagg ctgatccttg
     1981 gacaattttg ggtcaggtaa tttgtctttg tattgtctat tggtcatctt gcttcagttc
     2041 ttgtcatttg attttgtatt catctttttt tcttcaaatc tacagagttt cggtggcttt
     2101 tgtgcactta catatctgag ttttgcaccc gaaggactca aacaagtgct aataactgga
     2161 ggaattccac caattggtaa agcatgtaca gcagatgatg tatatgaagc cggttttgaa
     2221 caagttgctc gccaaaatga aaaatactac aagagattcc ctcaagacat tgagattgtt
     2281 cgtgaactcg taaattactt ggctgaatct gaaggtggcg gggtgaggga ttacaacatt
     2341 tgctcaatcc actgaccagt ttagtttagt tccttacttg tctctgtttc tttgtcttta
     2401 ggtacctctc ccatcaggag gcattcttac tcctaagggt cttcaaactc ttggtctttc
     2461 tggtttagga tcaagtactg gttttgagcg cctgcattat atgtaagagt ttagcttaat
     2521 ttttatcaac cacctcacat gttctgtgtt aaatctggcc ttcttaattg atcataggtt
     2581 ggagagagta tgggatccta ttttagttac tggagctcca aagtgtatta gtcagttctt
     2641 cttaaacgct gtaagagacc cacaaaaatt tgttgttttc tgttctcagc tcaaataagg
     2701 aattcaatag tctgagacat tttatcgttg ctgcagtttg agagttggca ttcttttgat
     2761 acaaatccac tatatgctct tctccacgag gccatatact gtgaggtata gtctcttcaa
     2821 atttctcatc tttttttcta tttcagacaa ttacttcttc ttgttatcgt ttatgtttaa
     2881 atggaaactt tttcagggtg cttcatcagg atggtctgct catagattac gggataaata
     2941 tgagtacaaa ttcgacgcaa tgaaggcagt caaagaaagt cagcctgtac tcttcacagg
     3001 agaggtacag gttttgagta tttctaattg tatggctcat agcttggatg attggtccac
     3061 attgcttaac taaaatagaa tttggatttg tatagatgat atttccatgg atgtttgatg
     3121 aaattcatgc cttgaagcca tttaaagctg ccgccgatct tttggccaag aaagaggatt
     3181 ggcctccatt atatgatgtt cctagattgc agaataacaa ggtctgtttc agaatcttga
     3241 tcgttctttc atatggatca aataatttta ctctgtttca catgatttct ttttgtaggt
     3301 gcctgttgct gcagcggttt acgaagaaga tatgtatgta aacttcaagc tggtgacgga
     3361 aacagcttct catatatcgg gtatcagact ttgggaaacg aatgagttta tgcattcggg
     3421 tcttcgtgat gctggaagac aaattattga tcatttgttg ggaatgatca atgggaaaaa
     3481 gcctctcttt tagggcgaat tcgacccagc tttcttgtac aaagtggtng ataacctaga
     3541 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     3601 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     3661 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     3721 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     3781 gcgcggtgtc atctatgtta ctagatcggg aattggttcc ggaaccaatt cgtaatcatg
     3841 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3901 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaggctga
     3961 attaggcgcg cctatttcta ttaattcccg atctagtaac atagatgaca ccgcgcgcga
     4021 taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg
     4081 cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat
     4141 tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg
     4201 attcaatctt aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgggggatc
     4261 gatcccgcga actgtggacg agaactgtgc caccaagcgt aaggccgttc tctcgcattt
     4321 gccttgctag gctcgcgcga gttgctggct gaggcgttct cgaaatcagc tcttgttcgg
     4381 tcggcatcta ctctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc
     4441 ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt
     4501 acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc
     4561 tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag
     4621 catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg
     4681 cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta
     4741 ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc
     4801 cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc
     4861 ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc
     4921 catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca
     4981 tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag
     5041 atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc
     5101 ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag
     5161 gctctcgctg aatgccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa
     5221 gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac
     5281 atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg
     5341 catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt
     5401 gagttcaggc tttttcatat cttattgccc cccgggatca gatctggatt gagagtgaat
     5461 atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca
     5521 agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc
     5581 tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg
     5641 ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat
     5701 aacgtgactc ccttaattct ccgctcatga tcttgatccc ctgcgccatc agatccttgg
     5761 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     5821 agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt
     5881 aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc
     5941 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt
     6001 ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctgatccgtc
     6061 gagattaata gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc
     6121 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     6181 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     6241 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     6301 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     6361 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt
     6421 cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     6481 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     6541 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     6601 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     6661 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc
     6721 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     6781 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     6841 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     6901 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     6961 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     7021 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     7081 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     7141 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     7201 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     7261 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     7321 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     7381 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     7441 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct
     7501 agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     7561 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     7621 cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa
     7681 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7741 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7801 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7861 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     7921 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7981 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8041 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8101 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8161 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8221 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8281 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8341 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8401 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8461 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     8521 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     8581 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     8641 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8701 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8761 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     8821 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     8881 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     8941 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     9001 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     9061 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     9121 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     9181 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     9241 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     9301 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     9361 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     9421 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     9481 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     9541 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     9601 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     9661 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     9721 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     9781 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
     9841 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
     9901 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
     9961 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
    10021 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
    10081 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
    10141 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
    10201 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
    10261 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
    10321 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
    10381 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
    10441 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
    10501 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
    10561 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
    10621 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
    10681 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
    10741 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
    10801 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
    10861 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
    10921 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
    10981 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
    11041 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
    11101 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    11161 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    11221 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    11281 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    11341 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    11401 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    11461 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    11521 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    11581 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    11641 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    11701 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    11761 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    11821 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    11881 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    11941 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    12001 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    12061 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    12121 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    12181 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    12241 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    12301 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    12361 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    12421 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    12481 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    12541 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    12601 catgcacata caaatggacg aacggataaa cct
//
