LOCUS       sequence-478302-        3881 bp    DNA     circular SYN 11-JUL-2025
DEFINITION  storage vector for BASIC part: RBS-RFP(ORF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_8_RBS34- mCherry -B0015
FEATURES             Location/Qualifiers
     source          1..3881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             19..30
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             37..747
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(182..200)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     450..469
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      767..838
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      854..881
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(954..973)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(955..973)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1279..1297)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1365..1469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1470..1538,1539..2330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1688..1707)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2501..3089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2990..3009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3243..3260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3380..3401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3416..3421,3422..3439,3440..3446)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 tctggtgggt ctctgtccaa agaggagaaa tactagatgg tgagcaaggg cgaggaggat
       61 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      121 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      181 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      241 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      301 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      361 gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta caaggtgaag
      421 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      481 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
      541 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
      601 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
      661 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      721 ggcggcatgg acgagctgta caagtaataa tactagagcc aggcatcaaa taaaacgaaa
      781 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      841 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttataggc tcgggagacc
      901 tatcgtatct tgctgaaaaa ctcgagccat ccggaagatc tggcggccgc tctccctata
      961 gtgagtcgta ttacgccgga tggatatggt gttcaggcac aagtgttaaa gcagttgatt
     1021 ttattcacta tgatgaaaaa aacaatgaat ggaacctgct ccaagttaaa aatagagata
     1081 ataccgaaaa ctcatcgagt agtaagatta gagataatac aacaataaaa aaatggttta
     1141 gaacttactc acagcgtgat gctactaatt gggacaattt tccagatgaa gtatcatcta
     1201 agaatttaaa tgaagaagac ttcagagctt ttgttaaaaa ttatttggca aaaataatat
     1261 aattcggctg caggggcggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1321 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1381 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1441 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1501 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1561 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1621 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1681 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1741 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1801 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1861 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1921 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1981 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2041 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2101 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2161 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2221 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2281 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2341 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2401 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2461 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2521 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2581 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2641 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2701 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2761 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2821 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2881 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2941 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3001 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3061 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3121 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3181 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3241 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3301 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcaattg
     3361 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     3421 actttatgct tccggctcgt ataatgtgtg gaattatgag cggataataa tttcacacag
     3481 gaggtttaaa ctttaaacat gtcaaaagag acgtcttttg ttaagaatgc tgaggaactt
     3541 gcaaagcaaa aaatggatgc tattaaccct gaactttctt caaaatttaa atttttaata
     3601 aaattcctgt ctcagtttcc tgaagcttgc tctaaacctc gttcaaaaaa aatgcagaat
     3661 aaagttggtc aagaggaaca tattgaatat ttagctcgta gttttcatga gagtcgattg
     3721 ccaagaaaac ccacgccacc tacaacggtt cctgatgagg tggttagcat agttcttaat
     3781 ataagtttta atatacagcc tgaaaatctt gagagaataa aagaagaaca tcgattttcc
     3841 atggcagctg agaatattgt aggagatctt ctagaaagat a
//
