LOCUS       sequence-478304-         845 bp    DNA     linear   UNA 14-JUL-2025
DEFINITION  bacterial expression of human TTR mutants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Sun X, Dyson HJ, Wright PE
  TITLE     Kinetic analysis of the multistep aggregation pathway of human 
            transthyretin.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Jul 3;115(27):E6201-E6208. doi: 
            10.1073/pnas.1807024115. Epub 2018 Jun 18.
  PUBMED    29915031
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1807024115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-07-3- 3"; volume: "115"; issue: "27"; pages: 
            "E6201-E6208"
COMMENT     Sequence Label: TTR C10S/S85C/V30M
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     174..193
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     281..300
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        281..299
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    300..324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             339..361
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(804..822)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
       61 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
      121 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
      181 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
      241 ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg
      301 gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag
      361 atatacatat gggtcctacg ggcaccggtg aatccaagag tcctctgatg gtcaaagttc
      421 tagatgctgt ccgaggcagt cctgccatca atgtggccat gcatgtgttc agaaaggctg
      481 ctgatgacac ctgggagcca tttgcctctg ggaaaaccag tgagtctgga gagctgcatg
      541 ggctcacaac tgaggaggaa tttgtagaag ggatatacaa agtggaaata gacaccaaat
      601 cttactggaa ggcacttggc atctgcccat tccatgagca tgcagaggtg gtattcacag
      661 ccaacgactc cggcccccgc cgctacacca ttgccgccct gctgagcccc tactcctatt
      721 ccaccacggc tgtcgtcacc aatcccaagg aatgagatcc ggctgctaca aagcccgaaa
      781 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      841 tgaac
//
