LOCUS       sequence-478306-         825 bp    DNA     linear   UNA 14-JUL-2025
DEFINITION  bacterial expression of TTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Sun X, Dyson HJ, Wright PE
  TITLE     Kinetic analysis of the multistep aggregation pathway of human 
            transthyretin.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Jul 3;115(27):E6201-E6208. doi: 
            10.1073/pnas.1807024115. Epub 2018 Jun 18.
  PUBMED    29915031
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1807024115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-07-3- 3"; volume: "115"; issue: "27"; pages: 
            "E6201-E6208"
COMMENT     Sequence Label: TTR C10S/S85C/L55P
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     154..173
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     261..280
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        261..279
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    280..304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             319..341
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(784..802)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
       61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
      121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
      181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
      241 cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
      301 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gggtcctacg
      361 ggcaccggtg aatccaagag tcctctgatg gtcaaagttc tagatgctgt ccgaggcagt
      421 cctgccatca atgtggccgt gcatgtgttc agaaaggctg ctgatgacac ctgggagcca
      481 tttgcctctg ggaaaaccag tgagtctgga gagccgcatg ggctcacaac tgaggaggaa
      541 tttgtagaag ggatatacaa agtggaaata gacaccaaat cttactggaa ggcacttggc
      601 atctgcccat tccatgagca tgcagaggtg gtattcacag ccaacgactc cggcccccgc
      661 cgctacacca ttgccgccct gctgagcccc tactcctatt ccaccacggc tgtcgtcacc
      721 aatcccaagg aatgagatcc ggctgctaca aagcccgaaa ggaagctgag ttggctgctg
      781 ccaccgctga gcaataacta gcataacccc ttggggcctc tgaac
//
