LOCUS       sequence-478308-         705 bp    DNA     linear   UNA 14-JUL-2025
DEFINITION  bacterial expression of TTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Sun X, Dyson HJ, Wright PE
  TITLE     Kinetic analysis of the multistep aggregation pathway of human 
            transthyretin.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Jul 3;115(27):E6201-E6208. doi: 
            10.1073/pnas.1807024115. Epub 2018 Jun 18.
  PUBMED    29915031
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1807024115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-07-3- 3"; volume: "115"; issue: "27"; pages: 
            "E6201-E6208"
COMMENT     Sequence Label: TTR C10S/S85C/T119M
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     141..160
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        141..159
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    160..184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             199..221
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(664..682)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
       61 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
      121 cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
      181 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gggtcctacg
      241 ggcaccggtg aatccaagag tcctctgatg gtcaaagttc tagatgctgt ccgaggcagt
      301 cctgccatca atgtggccgt gcatgtgttc agaaaggctg ctgatgacac ctgggagcca
      361 tttgcctctg ggaaaaccag tgagtctgga gagctgcatg ggctcacaac tgaggaggaa
      421 tttgtagaag ggatatacaa agtggaaata gacaccaaat cttactggaa ggcacttggc
      481 atctgcccat tccatgagca tgcagaggtg gtattcacag ccaacgactc cggcccccgc
      541 cgctacacca ttgccgccct gctgagcccc tactcctatt ccaccatggc tgtcgtcacc
      601 aatcccaagg aatgagatcc ggctgctaca aagcccgaaa ggaagctgag ttggctgctg
      661 ccaccgctga gcaataacta gcataacccc ttggggcctc tgaac
//
