LOCUS       sequence-47832-e         950 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  TITLE     SGC Q3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: USP37
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(407..424)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(434..451)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             470..492
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    507..531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(531..550)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(532..550)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(634..653)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttcgggcttg gttagcagcc ggaatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttcg tcatcacggt ttcatggctg caggaagtct gttttgatgg actgcatcta
      121 gaaacaacct catttcctct gcatccttac ttggtacttt gtcaatagac aagaagctgt
      181 tatcttgcag agttaacatt aggcggcttt gtttcgctcc actgggtcga agcaccacat
      241 ttttaatgtt atgacttagc tgaaatatcc ttggaattcc tccagtattg tagtgaacta
      301 ctaggctgac tttattctct ttttctacaa tttcaaagga tccttctttc cactttgtaa
      361 tcccagtctg catacttcga attctgatag gaccatgtat cttcagacta ccacgcggaa
      421 ccaggccgct gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta
      481 aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg
      541 agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg
      601 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
      661 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
      721 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
      781 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
      841 ggagagcgtc gagatcccgg acaccatcga atggcgcaaa acctttcgcg gtatggcatg
      901 atagcgcccg gaaaaaaagt caattcagtg tgatggacag actacagcca
//
