LOCUS       sequence-478351-        8924 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  Lentiviral CRISPR guide vector expressing a EGR3 sgRNA with cs1 
            incorporated in the loop of the sgRNA constant region for 10X Direct
            Capture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, 
            Guruvayurappan K, Norman TM
  TITLE     Comprehensive transcription factor perturbations recapitulate 
            fibroblast transcriptional states.
  JOURNAL   bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 
            10.1101/2024.07.31.606073.
  PUBMED    39131349
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 
            10.1101/2024.07.31.606073."
COMMENT     Sequence Label: pRCA860 - pBA904 Puro-T2A-GFP EGR3 g1 CRISPRa ...
FEATURES             Location/Qualifiers
     source          1..8924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(213..1004,1005..1073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     836..855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1074..1178)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1253..1272)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1444..1823
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1825..2023
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1978..1998
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2041..2221
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2268..2393
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2890..3123
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3308..3352
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3650..3767
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(4020..4053)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     4057..4077
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        4348..4559
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4560..5498
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5455..5475
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5519..6115
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5519..5538)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6015..6035
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6125..6178
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6185..6194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6191..6193,6194..6196,6197..6910)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6191..6193,6194..6196,6197..6907)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6236..6257)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6497..6516)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6844..6865
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6930..6963)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7019..7607
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7072..7092)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7490..7501)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7610..7626)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7611..7627)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8136..8316
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(join(8378..8924,1..42))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8458..8477)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
       61 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
      361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1201 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     1261 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     1321 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     1381 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     1441 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     1501 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1561 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1621 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1681 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     1741 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     1801 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     1861 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     1921 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     1981 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
     2041 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2101 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     2161 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     2221 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     2281 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     2341 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     2401 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     2461 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     2521 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     2581 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     2641 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     2701 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     2761 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     2821 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     2881 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     2941 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     3001 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     3061 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     3121 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     3181 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     3241 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     3301 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     3361 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     3421 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     3481 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     3541 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     3601 ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa
     3661 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     3721 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     3781 gggacagcag agatccagtt tggttagtac cgggcccgct ctagagatcc gacgcgccat
     3841 ctctaggccc gcgccggccc cctcgcacgg acttgtggga gaagctcggc tactcccctg
     3901 ccccggttaa tttgcatata atatttccta gtaactatag aggcttaatg tgcgataaaa
     3961 gacagataat ctgttctttt taatactagc tacattttac atgataggct tggatttcta
     4021 taacttcgta tagcatacat tatacgaagt tataaacagc acaaaaggaa actcacccta
     4081 actgtaaagt aattgtgtgt tttgagacta taagtatccc ttggagaacc accttgttgg
     4141 aaggggcgcg acgtcacaag tttaagagct aagctggaaa cagcatagca agtttaaata
     4201 aggctagtcc gttatcaact tggccgcttt aaggccggtc ctagcaaggc caagtggcac
     4261 cgagtcggtg ctttttttct cgagtactag gatccattag gcggccgcgt ggataaccgt
     4321 attaccgcca tgcatgtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     4381 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     4441 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     4501 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     4561 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     4621 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     4681 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     4741 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     4801 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     4861 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     4921 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     4981 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     5041 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     5101 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     5161 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     5221 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     5281 acctcgatta gttctcgcgc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     5341 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     5401 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     5461 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagct agcccaccat
     5521 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     5581 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     5641 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     5701 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     5761 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     5821 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     5881 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     5941 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6001 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6061 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccggatc
     6121 gggagagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccacc
     6181 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     6241 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     6301 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     6361 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     6421 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     6481 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     6541 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     6601 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     6661 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     6721 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     6781 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     6841 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     6901 gtacaagtaa gaattcgtcg agggacctaa taacttcgta tagcatacat tatacgaagt
     6961 tatacatgtt taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa
     7021 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7081 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7141 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7201 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7261 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7321 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7381 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     7441 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7501 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7561 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     7621 acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     7681 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     7741 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7801 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     7861 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     7921 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     7981 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     8041 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     8101 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     8161 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8221 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8281 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa
     8341 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8401 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8461 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8521 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8581 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8641 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8701 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8761 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8821 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8881 cttgatccgg caaacaaacc accgctggta gcggtggttt tttt
//
