LOCUS       sequence-478352-        8532 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  Expression of Flag- and His-tagged LARP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Shichino Y, Yamaguchi T, Kashiwagi K, Mito M, Takahashi M, Ito T, 
            Ingolia NT, Kuba K, Iwasaki S
  TITLE     eIF4A1 enhances LARP1-mediated translational repression during 
            mTORC1 inhibition.
  JOURNAL   Nat Struct Mol Biol. 2024 Oct;31(10):1557-1566. doi: 
            10.1038/s41594-024-01321-7. Epub 2024 May 21.
  PUBMED    38773334
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-024-01321-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2024-10"; volume: "31"; issue: "10"; pages: "1557-1566"
COMMENT     Sequence Label: pcDNA5/FRT/TO-3xFLAG-His6-LARP1
FEATURES             Location/Qualifiers
     source          1..8532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      979..988
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             988..1053
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1054..1071
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1072..1083
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4484..4501)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4490..4714
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4847..4866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4998..5045
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5053..6075
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5053..6075
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6205..6326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6242..6261)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6296..6315
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6375..6391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6375..6391)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6388..6410)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6399..6415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6423..6429,6430..6447,6448..6453))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6468..6489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6606..6623)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6777..7365)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6857..6876)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7536..8327,8328..8396))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8159..8178
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8397..8501)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttagc caccatggac tacaaagacc atgacggtga ttataaagat
     1021 catgacatcg attacaagga tgacgatgac aagcatcatc atcatcatca tatcgaaggt
     1081 aggcatatgg ccactcaggt ggagccgctg ctgcctgggg gcgccacgct cttgcaggcc
     1141 gaagagcacg gggggctggt gaggaagaag ccgccgccgg cgcccgaggg caagggcgag
     1201 cccgggccaa acgacgtccg cgggggggag ccggacggca gcgctcggag accccggccg
     1261 ccctgcgcca agccgcacaa ggagggcacc gggcagcagg agcgcgagag cccgcggccg
     1321 ctgcagctgc ccggcgccga aggcccggcc atcagcgacg gggaggaggg cggcggcgag
     1381 ccaggcgctg gcggaggagc tgccggagcc gcgggcgcgg ggcgccggga cttcgtggaa
     1441 gcccccccgc ccaaggtgaa cccgtggact aagaacgcat tgccgccggt cctgaccacc
     1501 gtgaacggac agtccccccc agaacactct gctccagcca aggtggtgag ggcagctgtt
     1561 cctaaacagc gcaaaggcag caaggttggt gactttggag atgcaatcaa ttggcccaca
     1621 cctggagaga tagcccacaa gagtgttcag ccacagtccc acaagcctca gcctacccgt
     1681 aaactgccac ccaagaagga catgaaggaa caggagaaag gagaagggag tgatagtaag
     1741 gagagtccaa aaaccaaatc agatgaatca ggggaggaaa agaatggaga tgaggattgc
     1801 cagcgaggcg ggcagaagaa gaaaggaaac aaacacaagt gggttccatt acaaatagac
     1861 atgaagcctg aagtgcccag agagaaactg gcttcacgcc ccactcgccc accggagcct
     1921 agacacatac ctgccaatcg cggagagatc aaagggtctg agtctgccac ctacgtgccc
     1981 gtggcccccc ccaccccagc ctggcaacca gagatcaaac cggagcctgc ctggcacgac
     2041 caggatgaga catcgagtgt gaagagtgat ggggctggtg gggcgcgggc ttccttccgt
     2101 ggccgtggac gggggcgtgg tcgcggccgg ggacgcggcc ggggtggcac tcgaacccat
     2161 tttgactacc agtttggcta ccgaaagttt gatggtgtgg aggggcctcg tacgcccaag
     2221 tacatgaaca acatcaccta ctactttgac aatgtcagca gcaccgagct ttacagtgtg
     2281 gatcaggaac tgctcaaaga ctacatcaag cgccagattg aatactactt cagcgtggac
     2341 aatttagagc gagacttctt cctgcgaagg aaaatggatg ctgatggttt cctacccatc
     2401 acccttattg cttccttcca ccgagtgcag gcccttacca ctgacatttc actcatcttt
     2461 gcggccctaa aggacagcaa ggtggtggag atcgttgatg agaaagttcg taggagggag
     2521 gaaccagaaa agtggcctct tcccccaata gtggattatt cacagactga tttctcccag
     2581 cttctcaact gccctgaatt tgttccccgt cagcactacc aaaaggagac agagtcggca
     2641 cctggctctc ctcgtgcagt caccccagtg ccaaccaaaa cagaggaggt cagcaaccta
     2701 aagacactac ccaagggcct gtctgccagc ctgcctgacc tggattctga gaactggatt
     2761 gaagtgaaga agaggcctcg gccatcccca gcacggccca agaagtcaga ggagtccaga
     2821 ttttcccacc tgacctctct gcctcagcag ctgccttccc agcagctgat gtccaaggat
     2881 caggatgagc aagaggaact ggattttctg tttgacgagg agatggagca gatggatggg
     2941 cggaagaaca ccttcactgc ctggtctgat gaggaatctg actatgagat tgatgacagg
     3001 gatgtcaaca agatcctcat tgtcacccag acaccacatt acatgcgccg gcacccaggg
     3061 ggggaccgca caggcaacca cacctcgcgt gccaagatga gcgccgaact ggccaaggtc
     3121 attaatgatg gcctcttcta ctatgagcag gacctgtggg ctgaaaagtt tgaacctgag
     3181 tattcccaga tcaagcaaga agtcgagaac ttcaaaaagg tcaatatgat cagccgggag
     3241 cagtttgaca cactgacccc tgagccccct gtggatccca accaggaagt tcctcctggg
     3301 ccacctcggt tccagcaagt tcctacggat gccctggcca acaagttgtt tggtgctcct
     3361 gagccctcca ccatcgcccg ctctctacca accactgtcc cagagtcacc aaactaccgc
     3421 aacaccagga cccctcgcac tccccggaca ccacagctca aagactcaag ccagacatca
     3481 cggttttacc cagtggtgaa agaaggacgg acactggatg ccaagatgcc tcgaaaaaga
     3541 aagacaagac acagttcaaa cccacccttg gagagccatg tgggctgggt gatggattcc
     3601 cgtgagcaca ggccccgtac tgcttccatc agctccagcc cctcagaagg gacgcctaca
     3661 gttggcagct atggctgtac ccctcagtca ttgcccaagt tccagcatcc ttcccatgaa
     3721 ctgctcaagg aaaatggctt cacacaacac gtctaccata agtatcgtag gcgctgcctt
     3781 aatgagcgga aacgcttggg cattggccag tctcaggaga tgaacacact cttccgcttc
     3841 tggtccttct tcctccgaga tcacttcaac aaaaagatgt atgaggagtt caagcagctg
     3901 gctctggagg acgccaaaga aggctacaga tatggtttgg agtgcctttt tcgatactac
     3961 agttatggcc tggaaaagaa gttccggctg gacatattca aggattttca ggaggaaacg
     4021 gtgaaggact atgaagctgg ccaactgtat gggctggaga agttctgggc cttcttgaaa
     4081 tattccaaag ccaaaaattt ggacattgac cccaaactgc aagaatacct cggcaaattc
     4141 cgacgtcttg aagacttccg agtagatccc cccatgggtg aggagggcaa ccacaagcga
     4201 cactcagtgg tagcaggagg tggcggcggt gagggcagga agcggtgccc ctcccagtct
     4261 tccagcaggc ctgctgccat gatcagccaa ccccctacac cacccaccgg ccagcctgtc
     4321 cgggaagatg ccaaatggac aagccagcac tcgaacacac agactttggg aaagtgatgg
     4381 taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg
     4441 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     4501 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4561 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4621 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     4681 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     4741 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4801 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4861 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4921 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4981 gatggttcac gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga
     5041 acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     5101 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     5161 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     5221 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     5281 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     5341 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     5401 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     5461 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     5521 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     5581 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     5641 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     5701 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     5761 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     5821 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     5881 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     5941 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     6001 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     6061 agggcaaagg aatagcacgt actacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6121 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6181 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     6241 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6301 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     6361 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6421 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6481 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6541 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6601 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6661 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6721 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6781 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6841 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6901 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6961 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7021 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7081 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7141 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7201 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7261 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7321 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7381 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7441 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7501 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7561 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7621 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7681 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7741 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7801 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7861 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7921 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7981 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8041 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8101 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8161 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8221 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8281 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8341 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8401 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8461 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8521 ccacctgacg tc
//
