LOCUS       sequence-478357-        7662 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  ScVip1-186-1107 protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Raia P, Lee K, Bartsch SM, Rico-Resendiz F, Portugal-Calisto D, 
            Vadas O, Panse VG, Fiedler D, Hothorn M
  TITLE     A small signaling domain controls PPIP5K phosphatase activity in 
            phosphate homeostasis.
  JOURNAL   Nat Commun. 2025 Feb 19;16(1):1753. doi: 10.1038/s41467-025-56937-0.
  PUBMED    39966396
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-02-19"; volume: "16(1):1753. doi"; pages: " 
            10.1038/s41467-025-56937-0"
COMMENT     Sequence Label: pFastBac1-ScVip1-186-1107
FEATURES             Location/Qualifiers
     source          1..7662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..36
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(46..69,70..105,106..129)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3115..3249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3152..3171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3206..3225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3278..3443
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3627..4081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3714..3733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3923..3944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4107..4211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4212..4280,4281..5072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4430..4449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5243..5831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5732..5751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5985..6002
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6136..6360
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6427..6960)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7149..7154,7155..7171,7172..7177))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        7529..7620
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     7545..7562
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 atgaagcatc atcatcatca tcaccatcac caccacggtg ctagctggtc ccacccccag
       61 ttcgagaagg gtggtggttc cggtggcgga tccggtggtt ctgcttggag ccaccctcaa
      121 ttcgaaaagg gctccggttc cggcgagaac ctgtacttcc aaggtgccaa agtgggcaag
      181 atcggtgtct gcgctatgga tgctaaggtg ctgtccaagc ctatgcgtca catcctgaac
      241 cgtctgatcg agcacggcga gttcgagact gtgatcttcg gcgacaaagt gatcctggac
      301 gagcgtatcg agaactggcc tacctgcgac ttcctgatct cattcttctc cagcggtttc
      361 ccactggaca aggccatcaa atacgtgaag ctgcgcaagc ccttcatcat caacgacctg
      421 atcatgcaga agatcctctg ggaccgtcgt ctgtgcctgc aagtgctcga ggcttacaac
      481 gtgcccactc ctcctcgtct ggaaatctcc cgtgatggtg gtccccgtgc taacgaggaa
      541 ctgcgtgcta agttgcgcga gcacggtgtc gaagtgaagc ccgtggaaga acccgagtgg
      601 aagatggtgg acgacgacac cttggaggtg gacggcaaga ccatgaccaa gcctttcgtc
      661 gagaagcctg tggacggcga ggaccacaac atctacatct actaccacag caagaacggt
      721 ggtggtggtc gccgtctgtt ccgtaaagtc ggcaacaagt cctccgagtt cgaccccact
      781 ctggtgcacc ctcgtaccga gggctcttac atctacgagc agttcatgga caccgacaac
      841 ttcgaggacg tgaaggctta tacaatcggc gagaacttct gccacgctga gactcgcaag
      901 tctcccgtgg tggacggtat cgtgcgtcgt aacacccacg gcaaagaagt gcgttacatc
      961 accgagctgt ctgacgagga aaagactatc gctggcaagg tgtccaaggc tttctcccag
     1021 atgatctgcg gtttcgacct gctgcgtgtg tccggcaagt cctacgtgat cgacgtgaac
     1081 ggtttcagct tcgtgaagga caacaaggcc tactacgact cctgcgctaa catcctgcgt
     1141 tccaccttca tcgaggccaa gaaaaagatg gacatggaaa agaagaactt gcccatcatc
     1201 cgcgaagaga aagaacagaa atgggtgttc aagggcctcg ccatcatcat ccgtcacgct
     1261 gaccgtactc ctaagcaaaa gttcaagcac tccttcacta gccccatctt catctccctg
     1321 ctgaagggtc acaaagagga agtcgtcatc cgtaacgtga acgatctgaa gatcgtgctg
     1381 caggctctgc gtatcgctct ggacgagaag gctggcaacc ccgctaagat caaggtgctg
     1441 gctaacgctc tcgagaagaa gctgaacttc cccggcacca agatccagct gaagccagtg
     1501 ctgaacaaag agaacgaggt cgagaaggtg cagttcatcc tgaagtgggg tggcgagcct
     1561 actcactccg ctaagtacca ggctaccgag ctgggagagc agatgcgtca agacttcgac
     1621 ctgttgaaca agtccatcct gcagaatatc aagatcttca gctcctccga gcgtcgtgtg
     1681 ctgcacactg ctcagtactg gacccgtgct ctgttcggtg ctgacgagct gggttccgac
     1741 gagatctcta tccgcaagga cctgctggac gactccaacg ctgctaagga cctcatggac
     1801 aaagtcaaga agaagttgaa gcccctgctg cgcgagggca aagaggctcc tcctcaattc
     1861 gcttggccct ccaagatgcc cgagccttac ctggtcatca agcgtgtggt cgagctgatg
     1921 aactaccaca agaaaatcat ggataacaac ttcgccaaga aagacgtgaa ctccatgcag
     1981 acccgttggt gcacctctga ggaccccagc ctgttcaaag agcgttggga caagcttttc
     2041 aaagagttca acaacgccga gaaggtcgac ccttccaaga tctccgagct gtacgacacc
     2101 atgaagtacg acgctctgca caaccgtcag ttcttggaga acatcttcga ccccggcctg
     2161 cctaacgagg ctatcgctga cgaactcggt tctcactccc tggtggatcg ttaccccatc
     2221 aacgtcctgg ccaagaacaa cttcaagatc atcgactccc actccatgaa caactccggc
     2281 aagaactcct ccaactccgt gggttctctc ggctgggtgc tcgagtctgg aaagacctca
     2341 accgctcgta accccaagtc ctctagccag ttcgacgagc cccgtttcat gcagctgcgc
     2401 gaactgtaca agctggctaa ggtcctgttc gacttcatct gccccaaaga gtacggtatc
     2461 tccgacgctg agaagctgga catcggcctg ctgacttctc tgcctctggc taagcagatc
     2521 ctgaacgaca tcggcgacat gaagaaccgc gagactcccg cttgtgtggc ttacttcacc
     2581 aaagagtccc acatctacac cctgctcaac atcatctacg aaagcggtat ccccatgagg
     2641 atcgctcgca acgctctgcc cgaactggac tacttgtccc agatcacctt cgagttgtac
     2701 gagtccaccg acgcttccgg ccagaagtcc cactctatcc gtctgaagat gtctcccggt
     2761 tgccacactc aggaccctct ggacgtgcaa ttggacgacc gtcactacat ctcttgcatc
     2821 cctaagatct ccctgaccaa gcacctcgac atggactacg tgcagcagaa gctgcgtaac
     2881 aagttcaccc gtgtgatcat gcctccaaag ttcactcccg tgaacatcac ctcataagcg
     2941 gccgctttcg aatctagagc ctgcagtctc gaggcatgcg gtaccaagct tgtcgagaag
     3001 tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     3061 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     3121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3241 gtctggatct gatcactgct tgagcctagg agatccgaac cagataagtg aaatctagtt
     3301 ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca
     3361 tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt
     3421 cccaactatt ttgtccgccc acagcggggc atttttcttc ctgttatgtt tttaatcaaa
     3481 catcctgcca actccatgtg acaaaccgtc atcttcggct actttttctc tgtcacagaa
     3541 tgaaaatttt tctgtcatct cttcgttatt aatgtttgta attgactgaa tatcaacgct
     3601 tatttgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     3661 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3721 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3781 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3841 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3901 gttggagtcc acgttcttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3961 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4021 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     4081 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4141 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4201 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4261 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4321 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4381 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4441 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4501 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4561 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4621 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4681 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4741 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4801 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4861 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4921 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4981 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5041 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5101 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5161 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5221 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5281 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5341 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5401 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5461 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5521 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5581 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5641 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5701 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5761 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5821 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5881 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5941 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6001 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6061 accgcataga ccagccgcgt aacctggcaa aatcggttac ggttgagtaa taaatggatg
     6121 ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa
     6181 ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat
     6241 gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag
     6301 tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa gactatattc
     6361 gcggcgttgt gacaatttac cgaacaactc cgcggccggg aagccgatct cggcttgaac
     6421 gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc
     6481 caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac
     6541 ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc
     6601 ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg
     6661 ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc
     6721 agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg
     6781 atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg
     6841 catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc
     6901 acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat
     6961 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     7021 cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
     7081 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
     7141 tgcattacag tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt
     7201 tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct
     7261 ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc
     7321 tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag atcggaagac
     7381 ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg
     7441 gttttctgga aggcgagcat cgtttgttcg cccaggactc tagctatagt tctagtggtt
     7501 ggctacgtat actccggaat attaatagat catggagata attaaaatga taaccatctc
     7561 gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat
     7621 attccggatt attcataccg tcccaccatc gggcgcggat cc
//
