LOCUS       sequence-478364-        2703 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  BTK Type 2-3 fluorescent protein cassette for golden gate assembly. 
            Improved version of pBTK1055.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Lariviere PJ, Ashraf AHMZ, Navarro-Escalante L, Leonard SP, Miller 
            LG, Moran NA, Barrick JE
  TITLE     One-step genome engineering in bee gut bacterial symbionts.
  JOURNAL   mBio. 2024 Sep 11;15(9):e0139224. doi: 10.1128/mbio.01392-24. Epub 
            2024 Aug 6.
  PUBMED    39105596
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2024-09-11"; volume: "15(9):e0139224. doi"; pages: " 
            10.1128/mbio.01392-24. Epub 2024 Aug 6"
COMMENT     Sequence Label: pBTK1129
FEATURES             Location/Qualifiers
     source          1..2703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(61..95)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(166..825)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     740..759
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1007..1595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1496..1515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggaaacacag aaaaaagccc acacctgaca gtgcgggctt ttttttcgac caaaggacca
       61 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
      121 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
      181 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
      241 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
      301 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
      361 actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt
      421 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
      481 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
      541 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
      601 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
      661 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
      721 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
      781 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
      841 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
      901 aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt
      961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1021 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1081 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1141 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1201 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1261 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1321 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1381 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1441 ggtatccggt aagcggcagg gtcgaaacag gagagcgcac gagggagctt ccagggggaa
     1501 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     1561 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     1621 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     1681 ctgtggataa ccgtacgttt gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt
     1741 actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag tcggtctcaa
     1801 acgctttggc agtttattct tgacatgtag tgagggggct ggtataatca catagttctg
     1861 ttatacagaa acagaggaga tattacatat gagtaaagga gaagagcttt tcacaggagt
     1921 tgtcccaatc ctcgtggaat tagacggtga tgttaatggg cacaagttct ctgtcagtgg
     1981 agagggtgaa ggcgacgcaa catatggcaa gctgaccctt aaatttattt gcaccacggg
     2041 taaactacct gttccatggc caacactggt cactacgttc gggtatgggg ttcagtgctt
     2101 tgcgcgctac ccagatcaca tgaaacagca cgactttttc aagagtgcaa tgcccgaagg
     2161 ctatgtacag gagagaacca tcttttttaa ggatgacggc aactataaga cacgcgccga
     2221 agtgaagttc gagggtgata cccttgttaa tagaatcgag ttaaagggta ttgactttaa
     2281 ggaagatgga aatattttag gccacaaact ggaatataac tataactccc ataatgtgta
     2341 cattatggcc gacaagcaaa agaacggtat caaggttaac ttcaagatca gacacaacat
     2401 tgaggatgga agcgttcaac tagccgacca ttaccaacaa aacaccccaa ttggcgatgg
     2461 gcctgtgctg ttaccagaca accattacct gtccactcaa tctgcccttt cgaaagatcc
     2521 caacgaaaag cgcgaccaca tggtccttct tgagtttgtc acggctgctg ggattacaca
     2581 cggcatggat gaactataca aataaatccg taatcgttaa tccgcaaata acgtaaaaac
     2641 ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt caatcctgag
     2701 acc
//
