LOCUS       sequence-478385-        3771 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  Plasmid insert contains a gene sequence for Human serum albumin for 
            use in Combinatorial Golden Gate Assembly (BsaI restriction sites)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J
  TITLE     High-throughput optimisation of protein secretion in yeast via an 
            engineered biosensor.
  JOURNAL   Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 
            10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13.
  PUBMED    39674781
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.tibtech.2024.11.010"; journalName: "Trends Biotechnol"; 
            date: "2025-04"; volume: "43"; issue: "4"; pages: "838-867"
COMMENT     Sequence Label: HSA_3b*_AP
FEATURES             Location/Qualifiers
     source          1..3771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(57..73)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(57..73)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(70..92)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(253..798)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(333..352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(906..1000)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1021..1680)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1595..1614
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1681..1783)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1870..1892
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1884..1901
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1885..1901
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca
       61 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat
      121 ccgacaatct ccaagacatt aggtggagtt cagttcggcg agcggaaatg gcttacgaac
      181 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
      241 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
      301 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
      361 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
      421 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
      481 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
      541 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
      601 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
      661 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
      721 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc
      781 agaccaaaac gatctcaaga agatcatctt attaagtctg acgctctatt caacaaagcc
      841 gccgtccatg ggtagggggc ttcaaatcgt ccccccatac gatataagtt gttactagtg
      901 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
      961 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
     1021 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     1081 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     1141 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     1201 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     1261 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     1321 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     1381 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     1441 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     1501 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     1561 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     1621 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     1681 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1741 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attgatacct
     1801 gaaacaaaac ccatcgtacg gccaaggaag tctccaataa ctgtgatcca ccacaagcgc
     1861 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tcatgcataa tccgcacgca
     1921 tctggaataa ggaagtgcca ttccgcctga cctgcatcgt ctcatcggtc tcaccctggt
     1981 gtattcagga gggatgccca taaatcagaa gttgcacatc gtttcaagga tttaggtgaa
     2041 gaaaatttca aggcacttgt tttgatagcg tttgcgcaat atctacaaca atgtccgttc
     2101 gaagatcatg taaaattagt aaatgaagtt acagaattcg ctaagacttg tgtggcagac
     2161 gagtccgcag agaactgtga taagtcattg catacccttt tcggtgataa gttatgtaca
     2221 gttgctactt tacgtgagac ttatggagaa atggctgact gttgcgcaaa gcaagaaccg
     2281 gagagaaacg aatgtttttt acaacataag gatgataacc ctaatttacc aagattggtc
     2341 cgtcctgaag tggatgtaat gtgcacagca tttcatgata atgaagaaac ttttctaaaa
     2401 aaatatcttt atgaaattgc taggcgacac ccttattttt acgctccaga actattgttt
     2461 tttgccaaac gctataaagc agcattcacg gaatgttgcc aagccgcaga caaggctgcc
     2521 tgtctattac caaagttgga tgaattaagg gacgaaggca aagcatcctc agctaaacaa
     2581 agactgaagt gtgcgtcttt acaaaaattc ggcgaaagag cctttaaggc ttgggcagtc
     2641 gcgagattgt cgcagagatt ccccaaagcg gaatttgccg aagtctcgaa actcgttact
     2701 gatctaacca aagttcatac tgaatgctgc cacggggatt tactggaatg tgccgatgac
     2761 agagcggacc ttgctaaata catttgtgag aatcaggatt ctataagttc aaagttgaag
     2821 gaatgttgtg agaaaccttt actagagaaa tcccactgca tcgctgaggt cgaaaatgat
     2881 gagatgccag cggatcttcc gagcttggcc gctgattttg tggaaagtaa agacgtctgt
     2941 aagaattatg cagaagcaaa agatgtattt ttgggtatgt tcttgtacga gtacgctaga
     3001 aggcatcccg actactctgt tgtactgttg cttcgattgg caaaaacata cgaaacaacg
     3061 ctggagaaat gttgtgcagc tgccgatcct cacgaatgct acgccaaagt ctttgacgaa
     3121 tttaagccac ttgttgagga acctcaaaac cttataaagc agaactgtga actatttgaa
     3181 caactgggtg aatacaaatt tcaaaatgcc ttgttagttc gctatactaa gaaagttcca
     3241 caagtttcta cacccacgtt agttgaagtt tctcgtaact tgggaaaagt ggggtcaaag
     3301 tgttgtaagc atccagaagc taaaagaatg ccctgtgcgg aagattattt gtctgtggta
     3361 ttgaatcagt tatgcgtact acatgaaaag actcctgtga gtgacagagt tacaaaatgc
     3421 tgcacggaga gtttggtgaa ccggcgtcca tgcttttctg cactggaagt cgacgagaca
     3481 tatgtgccta aagagtttaa tgctgagact tttaccttcc atgccgatat ttgcacctta
     3541 tcggagaaag aaagacagat caaaaagcag accgctttag ttgaattggt caaacacaaa
     3601 ccaaaagcca caaaagaaca gttaaaggca gtgatggatg attttgctgc tttcgtcgag
     3661 aagtgttgta aagccgacga caaagaaact tgcttcgctg aggaaggtaa gaaattggta
     3721 gctgcttcac aagctgccct gggcctcgga tcctgagacc tgagacggca t
//
